The clonal cherry rootstock CAB-6P (Prunus cerasus L.) is a commercial cherry rootstock with limited practical use compared to Prunus avium L. and Prunus mahaleb (Dimassi-Theriou, Therios 2006). In general, sour cherry as a rootstock is reported to be winter-hardy, tolerant to wet soils but inconsistent compatibility with some sweet cherry cultivars. CAB-6P is a rootstock suitable for sweet and sour cherry varieties. Trees in the nursery are moderately vigorous and have an intermediate growth habit. The root system is moderately developed with good anchorage in the ground; tendency to suckering. It adapts well to heavy soils with low permeability, it is resistant to calcareous soils (9% active lime) as well as to nematodes and it is suitable for replanting.Gisela 6 is a hybrid of Prunus cerasus × Prunus canescens and used as a semi-dwarf rootstock for all kinds of sweet cherry. It is also satisfactory in a wide range of soils, especially heavy soils (Andersen et al. 1999). The average and slow growth of this rootstock has a great value for the establishment and development of modern intensive cherry orchards. Trees grafted on this rootstock do not have any suckering problems (Long, Kaiser 2010). Producing Gisela 6 rootstock in large scale using conventional methods such as cuttings and layering in order to meet the growing internal demand The effects of two strength media (full and half ) in inorganics and five polyvinylpyrrolidone (PVP) concentrations combined with 1 mg/l indole-3-butyric acid on the in vitro rooting of the CAB-6P, Gisela 6 and M × M 14 cherry rootstocks were investigated. For the CAB-6P, root number (5.80) was greatest by adding 5 g/l PVP to the half strength medium and 1 g/l PVP resulted in 80% rooting percentage. For Gisela 6, 1 g/l PVP in half strength medium led to the highest root number (6.30) as well as the highest rooting percentage (90.91%). On the contrary, root length was maximum (36.17 mm) in the full strength medium without adding PVP. In M × M 14, root number was maximum (8.33) in the PVP-free full strength medium, rooting percentage was 100% by adding 5 g/l PVP to either full or half strength medium and root length was greatest by incorporating 1 or 2.5 g/l PVP into the half strength medium. PVP seems to be a promising rooting agent in tissue culture systems.