Effectiveness of an Ozone Disinfecting and Sanitizing Cabinet to Decontaminate a Surrogate Virus for SARS-CoV-2 on N-95 Masks

Beaudry, Marie-Christine; Jc, Frederick; Mej, Lott; Norfolk, William A.; Glenn, Travis C.; Ek, Lipp

doi:10.1101/2020.11.04.20226233

Cited by 5 publications

(7 citation statements)

References 10 publications

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“…1) indicate the effectiveness of the ozone-chamber procedure to inactivate both Gram-negative and Gram-positive bacteria. Interestingly, our findings demonstrated that the Gram-positive S. pseudintermedius LBV072/17 was undetectable in a shorter amount of ozone time exposure than the Gram-negative E. coli ATCC 25922, which is in contrast with previous literature that determined a higher ozone sensibility in Gram-negative bacteria [17,21]. Similar results were obtained with face masks experimentally contaminated with influenza A (H 1 N 1 ) virus and Gram-positive Staphylococcus aureus bacteria.…”

Section: Rapid Ozone-based Treatment Is Effective To Disinfect Gram-n...supporting

confidence: 64%

Inactivation of Pathogenic Microorganisms on Polymeric Materials through Ozonation

et al. 2023

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Ozone-based technologies have been evaluated to inactivate the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on surfaces. However, the vast diversity of information makes it difficult to establish common ground for determining the best practices for using this technology. The objective of this work is to evaluate the success of N95 mask decontamination by ozonation, determining the specific parameters for process control. To quantify the effectiveness of the process, a disinfection protocol was initially developed based on two bacterial species (Escherichia coli and Staphylococcus pseudintermedius), followed by another disinfection assay using the murine hepatitis coronavirus (MHV-3), in a laboratory-scale prototype. Ozone is an effective candidate for use against SARS-CoV-2 or other viruses to disinfect personal protection equipment (PPE).

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Section: Rapid Ozone-based Treatment Is Effective To Disinfect Gram-n...supporting

confidence: 64%

Inactivation of Pathogenic Microorganisms on Polymeric Materials through Ozonation

et al. 2023

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“…Our study was primarily directed at discovering the potential of ozone in the disinfection of PPE. The promising data from this small-scale investigation on a SARS-CoV2 surrogate virus -MS2 bacteriophage [16] -suggests further studies that may compare ozone to currently employed decontaminants, such as VHP.…”

Section: Discussionmentioning

confidence: 96%

“…Bacterial cells including E. coli (strain C-3000) serve as a host for MS2 bacteriophage. Its small size, icosahedral structure, and environmental resistance have made MS2 ideal for use as a surrogate virus (particularly in place of picornaviruses such as poliovirus and human norovirus) in water quality and disinfectant studies [13][14][15][16]. The respective incubation and phage propagation procedures were obtained from the supporting documentation for each product, and all incubation media were tested for and passed standard sterility tests.…”

Section: Test Microorganismmentioning

confidence: 99%

Ozone: A Novel Sterilizer for Personal Protective Equipment

Stolle¹,

Nalamasu²,

Rodenbeck³

et al. 2021

Cureus

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Objective: Personal protective equipment (PPE) is urgently sought during public health crises. It is necessary for the safety of both the patient and the healthcare professional. Yet during the recent COVID-19 pandemic, PPE scarcity in many countries, including the United States, has impacted the level of care for patients and the safety of healthcare personnel. Additionally, the implementation of mandatory mask mandates for the general public in many countries forced individuals to either reuse PPE, which can contribute to poor hygiene, or buy PPE in bulk and thereby contribute to the scarcity of PPE. In this study, we investigate the possibility of using a cost-effective ozone sterilization unit on contaminated N95 masks as an alternative to current sterilization methods.Method: This protocol examined ozone's ability to decontaminate N95 mask fabric that was exposed to a surrogate virus (Escherichia coli bacteriophage MS2). Once the sterilization unit achieves an ozone concentration of ~30 ppm, a 60-minute or 120-minute sterilization cycle commences. Following the sterilization cycle, we investigated the amount of viable virus on the slide using a viral plaque assay and compared it to a non-sterilized, control slide. Furthermore, we carried out trials to investigate the safety of an ozone sterilization device, by measuring the levels of ozone exposure that individuals may experience when operating the sterilization unit post-cycle.Results: We showed that a 120-minute sterilization cycle at ~30 ppm achieves a 3-log reduction in viral activity, thereby complying with industry and U.S. Food and Drug Administration (FDA) standards. Further, we demonstrated that when following our protocol, the ozone exposure levels for a simple sterilization unit to be used at home complied with federal and industry standards. Conclusion:Ozone may have the potential to decontaminate masks and other PPE.

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“…Since all these methods have advantages and disadvantages, a careful consideration of the type of respirator and biological target has to be performed, to restore the fit and filtration capacity of the N95 respirator post decontamination. In this regard, several recent reports have evaluated the effect of ozone treatment on the filtration performance of N95 respirators [ 46 , 72 , 73 , 74 ]. Interestingly, neither higher ozone concentration (200 ppm for 90 min) nor prolonged ozone exposure (20 ppm for 36 h) appear to degrade or damage the filtration efficiency of N95 FFRs, illustrating the effectiveness of ozone treatment for contaminated FFRs regeneration [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

Inactivation of Human Coronavirus by FATHHOME’s Dry Sanitizer Device: Rapid and Eco-Friendly Ozone-Based Disinfection of SARS-CoV-2

Uppal

Khazaieli²,

Snijders

et al. 2021

Pathogens

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The pandemic of SARS-CoV-2/COVID-19 was reported in December 2019 in Wuhan, China. Pertaining to its high transmissibility and wide host adaptability, this unique human coronavirus spread across the planet inflicting 115 million people and causing 2.5 million deaths (as of March 3rd, 2021). Limited or negligible pre-existing immunity to multiple SARS-CoV-2 variants has resulted in severe morbidity and mortality worldwide, as well as a record-breaking surge in the use of medical-surgical supplies and personal protective equipment. In response to the global need for effective sterilization techniques, this study evaluated the virucidal efficacy of FATHHOME’s self-contained, ozone-based dry-sanitizing device, by dose and time response assessment. We tested inactivation of human coronavirus, HCoV-OC43, a close genetic model of SARS-CoV-2, on porous (N95 filtering facepiece respirator/FFR) and nonporous (glass) surfaces. We started our assays with 20 ppm-10 min ozone exposure, and effectively reduced 99.8% and 99.9% of virus from glass and N95 FFR surfaces, respectively. Importantly, the virus was completely inactivated, below the detection limit (over 6-log10 reduction) with 25 ppm-15 min ozone exposure on both tested surfaces. As expected, a higher ozone exposure (50 ppm-10 min) resulted in faster inactivation of HCoV-OC43 with 100% inactivation from both the surfaces, with no residual ozone present after completion of the 5-min post exposure recapture cycle and no measurable increase in ambient ozone levels. These results confirmed that FATHHOME’s device is suitable for rapid decontamination of SARS-CoV-2- from worn items, frequently touched items, and PPE including N95 FFRs, face shields, and other personal items.

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Effectiveness of an Ozone Disinfecting and Sanitizing Cabinet to Decontaminate a Surrogate Virus for SARS-CoV-2 on N-95 Masks

Cited by 5 publications

References 10 publications

Inactivation of Pathogenic Microorganisms on Polymeric Materials through Ozonation

Inactivation of Pathogenic Microorganisms on Polymeric Materials through Ozonation

Ozone: A Novel Sterilizer for Personal Protective Equipment

Inactivation of Human Coronavirus by FATHHOME’s Dry Sanitizer Device: Rapid and Eco-Friendly Ozone-Based Disinfection of SARS-CoV-2

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