Effectiveness of an Innovative Pulsed Electromagnetic Fields Stimulation in Healing of Untreatable Skin Ulcers in the Frail Elderly: Two Case Reports

Guerriero, F.; Botarelli, Emanuele; Mele, Gianni; Polo, Lorenzo; Zoncu, Daniele; Renati, Paolo; Sgarlata, C.; Rollone, M.; Ricevuti, Giovannoi; Maurizi, Niccolò; Francis, Marybeth; Rondanelli, Mariangela; Perna, Simone; Guido, Davide; Mannu, Piero

doi:10.1155/2015/576580

Cited by 12 publications

(15 citation statements)

References 18 publications

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“…Several clinical studies have demonstrated that PEMFs can effectively alleviate tissue swell, traumatic pain and promote wound healing ( 19 , 20 ). Guerriero et al ( 21 ) described the first reported case of an innovative PEMF therapy, emysimmetric bilateral stimulation (EBS), used to successfully treat refractory skin ulcers in two elderly and fragile patients. They demonstrated a supportive role of PEMFs in the treatment of skin ulceration in diabetes, which was suggestive of a potential benefit of EBS for this clinical condition.…”

Section: Discussionmentioning

confidence: 99%

Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro

Cheng¹,

Qu²,

Fu³

et al. 2017

Molecular Medicine Reports

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A variety of pulsed electromagnetic fields (PEMFs) have been experimentally and clinically used in an effort to promote wound healing, although the mechanisms involved remain unknown. The aim of the present study was to investigate the action of a novel protocol of co-treatment with PEMFs and hydroxytyrosol (HTY) on the proliferation and differentiation potential of human umbilical vein endothelial cells (HUVECs). The HUVECs were assigned randomly into three groups: Control, PEMF-treated and PEMF + HT-treated. The intensity of the electromagnetic field used in this protocol was 2.25 mT, the frequency of the bursts was 50 Hz and the application time was 15 min. A Cell Counting kit-8 (CCK-8) assay was used to assess cell proliferation, and cell apoptosis was analyzed by TUNEL apoptosis assay kit and calcein-acetoxymethyl/propidium iodide dual-staining assay. In addition, protein and mRNA expression levels of protein kinase B (Akt), mechanistic target of rapamycin (mTOR), transforming growth factor (TGF)-β1 and p53 were determined by western blotting and reverse transcription-quantitative polymerase chain reaction assays, respectively. The CCK-8 assay demonstrated that HTY contributed to HUVEC proliferation mediated by PEMFs in a time-dependent manner. The Transwell assay and scratch wound results demonstrated that co-treatment of HTY and PEMFs could increase HUVEC migration. Furthermore, the levels of apoptotic cells were reversed by pre-incubation with HTY in the PEMF treatment group, while PEMF treatment alone had no such effect. The proteins and mRNA expression levels of Akt, mTOR, TGF-β1 were elevated in co-treatment of HTY and PEMFs, whereas there was no effect on levels of p53. Therefore, the results indicated that combined exposure of HUVECs to PEMFs and HTY exerted protective effects in HUVECs by promoting cell proliferation and inhibiting apoptosis. In conclusion, to the best of our knowledge, the present study was the first to demonstrate the beneficial roles of HTY and PEMF combined treatment in HUVECs, which may represent an effective treatment for wound healing.

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Section: Discussionmentioning

confidence: 99%

Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro

Cheng¹,

Qu²,

Fu³

et al. 2017

Molecular Medicine Reports

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“…Although many studies have shown the effect of magnetic fields on human and animal model, [32][33][34][35][36][37] further and more comprehensive studies are still required. This study revealed that EMF might serve as a potential tool for manipulating activity of cells and it opens a new investigation field valuable for future new therapeutic approach to accelerate skin regeneration and wounds closure.…”

Section: Inhibitorsmentioning

confidence: 99%

Extremely low‐frequency electromagnetic fields accelerates wound healing modulating MMP‐9 and inflammatory cytokines

et al. 2018

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“…More recently, a reduction of pain and wound inflammation in women undergoing cesarean surgery was reported ( 49 ). No adverse effects were observed in any of the studies ( 47 , 48 , 56 , 120 ). Although all these in vivo studies and clinical observations showed positive results, the real picture is more complex because there is still a fraction of patients that does not respond with improved healing.…”

Section: Emf and Immunomodulation In Wound Healingmentioning

confidence: 83%

Immune-Modulating Perspectives for Low Frequency Electromagnetic Fields in Innate Immunity

Rosado¹,

Simkó²,

Mattsson

et al. 2018

Front. Public Health

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In recent years, the effects of electromagnetic fields (EMFs) on the immune system have received a considerable interest, not only to investigate possible negative health impact but also to explore the possibility to favorably modulate immune responses. To generate beneficial responses, the immune system should eradicate pathogens while “respecting” the organism and tolerating irrelevant antigens. According to the current view, damage-associated molecules released by infected or injured cells, or secreted by innate immune cells generate danger signals activating an immune response. These signals are also relevant to the subsequent activation of homeostatic mechanisms that control the immune response in pro- or anti-inflammatory reactions, a feature that allows modulation by therapeutic treatments. In the present review, we describe and discuss the effects of extremely low frequency (ELF)-EMF and pulsed EMF on cell signals and factors relevant to the activation of danger signals and innate immunity cells. By discussing the EMF modulating effects on cell functions, we envisage the use of EMF as a therapeutic agent to regulate immune responses associated with wound healing.

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Effectiveness of an Innovative Pulsed Electromagnetic Fields Stimulation in Healing of Untreatable Skin Ulcers in the Frail Elderly: Two Case Reports

Cited by 12 publications

References 18 publications

Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro

Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro

Extremely low‐frequency electromagnetic fields accelerates wound healing modulating MMP‐9 and inflammatory cytokines

Immune-Modulating Perspectives for Low Frequency Electromagnetic Fields in Innate Immunity

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