Extremely low‐frequency electromagnetic fields accelerates wound healing modulating <scp>MMP</scp>‐9 and inflammatory cytokines

Patruno, Antonia; Ferrone, Alessio; Costantini, Erica; Franceschelli, Sara; Pesce, Mirko; Speranza, Lorenza; Amerio, Paolo; D’Angelo, Chiara; Felaco, Mario; Grilli, Alfredo; Reale, Marcella

doi:10.1111/cpr.12432

Cited by 52 publications

(40 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[16][17][18] These mechanical stresses also stimulate keratinocytes to produce pro-inflammatory cyto-/ chemokines, including TNF-a, IL-1a and CXCL8, by a process partly shared with biochemical stimuli. [16][17][18][19][20] Psoriasis and atopic dermatitis (AD) are common inflammatory skin diseases, and their development and disease activities are affected by external stimuli. In psoriasis, scratch injury is frequently associated with the development of a new lesion, which is called the Koebner phenomenon.…”

Section: Introductionmentioning

confidence: 99%

Pathogenic implication of epidermal scratch injury in psoriasis and atopic dermatitis

Furue

Ulzii

Tanaka

et al. 2020

The Journal of Dermatology

View full text Add to dashboard Cite

Mechanical scratching, a common external stress affecting the skin, is induced by various causes, such as pruritus. Scratch injury to epidermal keratinocytes upregulates the production and release of chemokine (C‐C motif) ligand 20 (CCL20) in vitro, which selectively chemoattracts interleukin (IL)‐17A‐producing immune cells that express chemokine (C‐C motif) receptor 6 (CCR6). In IL‐17A‐dominant psoriasis, scratch‐induced CCL20 upregulation and subsequent accumulation of IL‐17A‐producing immune cells and CCR6+ mature dendritic cells may trigger the development of psoriatic lesions, a process known as the Koebner phenomenon. In IL‐4/IL‐13‐dominant atopic dermatitis, pruritus and subsequent scratching are the primary symptoms. Scratch‐induced CCL20 production from keratinocytes may explain why IL‐17A levels are also elevated in atopic dermatitis. In contrast, mechanical scratching is likely to negatively regulate IL‐13 signaling by upregulating the expression of IL‐13 receptor α2, which serves as a decoy receptor for IL‐13 in keratinocytes. In this review, we summarize current reports on topics related to the pathogenic role of epidermal scratch injury in psoriasis and atopic dermatitis.

show abstract

Section: Introductionmentioning

confidence: 99%

Pathogenic implication of epidermal scratch injury in psoriasis and atopic dermatitis

Furue

Ulzii

Tanaka

et al. 2020

The Journal of Dermatology

View full text Add to dashboard Cite

show abstract

“…In in vitro experiments carried out in our laboratory, we found that HaCaT cells exposed to 50 Hz, 1 mT flux density, already upregulate matrix metalloprotease (MMP) 9 level at 1 h, peaking at 8 h, and decreasing at 24 h, in accordance to the wound healing [ 7 ]. The increase of the canonical pathway of the mammalian target of rapamycin (mTOR) regulation phosphoinositide 3-kinases/protein kinase B (PI3K/Akt) and activation of extracellular signal-regulated kinases (Erk) signaling pathways were detected in HaCaT cells exposed to 50 Hz, 1 mT, for 1–3 h [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells

Patruno

Costantini

Ferrone

et al. 2020

IJMS

View full text Add to dashboard Cite

Extremely low frequency electromagnetic fields (ELF-EMFs) have been known to modulate inflammatory responses by targeting signal transduction pathways and influencing cellular redox balance through the generation of oxidants and antioxidants. Here, we studied the molecular mechanism underlying the anti-oxidative effect of ELF-EMF in THP-1 cells, particularly with respect to antioxidant enzymes, such as heme oxygenase-1 (HO-1), regulated transcriptionally through nuclear factor E2-related factor 2 (Nrf2) activation. Cells treated with lipopolysaccharides (LPS) were exposed to a 50 Hz, 1 mT extremely low frequency electromagnetic fields for 1 h, 6 h and, 24 h. Our results indicate that ELF-EMF induced HO-1 mRNA and protein expression in LPS-treated THP-1 cells, with peak expression at 6 h, accompanied with a concomitant migration to the nucleus of a truncated HO-1 protein form. The immunostaining analysis further verified a nuclear enrichment of HO-1. Moreover, ELF-EMF inhibited the protein expressions of the sirtuin1 (SIRT1) and nuclear factor kappa B (NF-kB) pathways, confirming their anti-inflammatory/antioxidative role. Pretreatment with LY294002 (Akt inhibitor) and PD980559 (ERK inhibitor) inhibited LPS-induced Nrf2 nuclear translocation and HO-1 protein expression in ELF-EMF-exposed cells. Taken together, our results suggest that short ELF-EMF exposure exerts a protective role in THP-1 cells treated with an inflammatory/oxidative insult such as LPS, via the regulation of Nrf-2/HO-1 and SIRT1 /NF-kB pathways associated with intracellular glutathione (GSH) accumulation.

show abstract

“…MMP2 and MMP9 are essential for the initiation of bone remodeling, responsible for managing acute and chronic inflammatory responses [40,41]. In the present study, an increase in MMP9 levels was detected in periodontopathic patients rather than in healthy subjects.…”

Section: Discussionmentioning

confidence: 39%

Evaluation of Salivary Cytokines and Vitamin D Levels in Periodontopathic Patients

Costantini

Sinjari

Piscopo

et al. 2020

IJMS

View full text Add to dashboard Cite

Periodontal disease (PD) is an inflammatory condition of the tissues supporting the teeth, which is widespread among the adult population. Evidence shows a relationship between PD and vitamin D levels, which is involved in the regulation of bone metabolism, mineral homeostasis, and inflammatory response. This study aimed to perform a simultaneous evaluation of inflammatory mediators and vitamin D levels in saliva in periodontopathic patients to better understand their role in periodontal disease. In this observational study, clinical periodontal parameter examination was performed for each patient. Moreover, the saliva levels of 25(OH)D3, TGFβ, IL-35, IL-17A, and MMP9 were evaluated using an ELISA assay. An increase in TGFβ, IL-35, MMP9, and IL-17A salivary levels and a reduction in 25(OH)D3 levels were observed in periodontopathic patients with respect to the healthy controls. The present study revealed significant positive correlation between cytokines and highly negative correlation between 25(OH)D3 and salivary cytokine levels. Further studies are needed to better understand if salivary cytokines and vitamin D evaluation may represent a new approach for detection and prevention of progressive diseases, such as PD.

show abstract

Extremely low‐frequency electromagnetic fields accelerates wound healing modulating MMP‐9 and inflammatory cytokines

Abstract: Our results highlight ability of ELF-EMF to modulate inflammation mediators and keratinocyte proliferation/migration, playing an important role in wound repair. The ELF-EMF accelerates wound healing modulating expression of the MMP-9 via Akt/ERK pathway.

Cited by 52 publications

References 35 publications

Pathogenic implication of epidermal scratch injury in psoriasis and atopic dermatitis

Pathogenic implication of epidermal scratch injury in psoriasis and atopic dermatitis

Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells

Evaluation of Salivary Cytokines and Vitamin D Levels in Periodontopathic Patients

Contact Info

Product

Resources

About