Effectiveness of a bioabsorbable conduit in the repair of peripheral nerves

Aldini, N. Nicoli; Perego, G.; Cella, Gian Domenico; Maltarello, Maria Cristina; Fini, Milena; Rocca, Michele; Giardino, Roberto

doi:10.1016/0142-9612(96)84669-2

Cited by 111 publications

(58 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A confecção das membranas de PCL e PLLA foram feitas a partir da técnica do solvente [31] , de acordo com a metodologia apresentada na confecção das membranas. Devido a sua leveza, foram colocadas arruelas de titânio sobre a biomembranas a fim de evitar que ocorresse a flutuação destas durante e após a colocação das células do neuroblastoma e o meio de cultura.…”

Section: Resultsunclassified

“…Nesse intuito, vários biomateriais foram testados ao longo dos anos, entre eles, o poli ácido lático (PLLA), poli caprolactona (PCL), chitosana e poliuretano [9,16,31,33] . O estudo dos biomateriais ora supracitados, entre outros, foram pesquisados não somente in vivo [14,16,34] , mas in vitro, com os diversos tipos celulares, tais como os fibroblastos, as células de Schwann e neurônios [2,11,13,16,35] .…”

Section: Resultsunclassified

“…As membranas de PCL e PLLA foram confeccionadas a partir da técnica do solvente [31] . Para isto, as amostras foram colocadas separadamente em 33ml de solvente (dicloroetano) (Merck, Darmstadt, Alemanha), de modo que as concentrações finais fossem 5% e 2,5% respectivamente (peso/volume).…”

Section: Confecção De Membranas De Pcl E Pllaunclassified

See 2 more Smart Citations

Estudo das células Neuro2A sobre os biomateriais PCL e PLLA

et al. 2014

View full text Add to dashboard Cite

Resumo: Os biomateriais poli L-ácido lático (PLLA) e o poli caprolactona (PCL) são os polímeros mais estudadas na área dos materiais bioreabsorvíveis. Dentre as suas principais características que contribuem para a interação celular, temos a especificidade química da superfície, elétrica, hidrofobicidade e topografia. Ainda, observa-se o tempo de degradação, porosidade, biocompatibilidade com o tecido biológico, bem como, a confecção com as mais variadas formas e dimensões. Já a prática da cultura celular, tem como objetivo estudar a adesão, migração, diferenciação e a proliferação celular utilizando-se um determinado material ou substância. Contudo, poucos trabalhos utilizando os biomateriais ora supracitados e a aplicação em células neuro2A foram realizados. Sabe-se que este tipo celular é derivado de células embrionárias da crista neural, as quais originam em neurônios simpáticos e apresentam como característica a imortalidade, portanto, são excelentes modelos em ensaios in vitro. Nesse sentido, o presente estudo avalia a adesão e a proliferação desta linhagem celular sobre os biopolímeros poli caprolactona (PCL) e poli L-ácido lático (PLLA). Palavras-chave: Biomateriais, PLLA, PLC, neuroblastoma, cultura. Study of Neuro2a Cells on the Biomaterials Poly L-lactic Acid and Poly CaprolactoneAbstract: Biomaterials poly L-lactic acid (PLLA) and poly caprolactone (PCL) are the most studied in the area of bioresorbable materials. Among the main features that contribute to cell interaction, we have the specific surface chemistry, electrical, hydrophobicity and topography. Also, is observed the degradation time, porosity, biocompatibility with biological tissue, as well as the preparation of the most varied shapes and sizes. The practice of cell culture, aims to study the adhesion, migration, differentiation and cell proliferation using a given material or substance. However, few studies were performed using these biomaterials and the application of neuro2A cells. It is known that this cell type is derived from the embryonic neural crest cells, which originate in sympathetic neurons and have the characteristic of immortality, therefore, are excellent models in vitro assays. Accordingly, the present study evaluates the adhesion and proliferation of this cell line on the biopolymers poly caprolactone (PCL) and poly L-lactic acid (PLLA). Glória, 187, Centro, Diamantina, MG, Brasil, Keywords: Biomaterials, PLLA, PLC, neuroblastoma, culture. Autor para correspondência: Amauri Pierucci, Departamento de Ciências Básicas, Universidade Federal dos Vales do Jequitinhonha e Mucuri -UFVJM, Rua da IntroduçãoCom o advento da bioengenharia intensificaram os estudos sobre a aplicação e ação dos biomateriais em diferentes tipos celulares. Sabe-se que a adesão, migração, diferenciação e a proliferação celular sobre determinado substrato depende em parte das propriedades intrínsecas de cada tipo celular, bem como, o tipo de material utilizado [1][2][3][4] . Dentre os produtos celulares, pode-se ressaltar a síntese de moléculas de adesã...

show abstract

Section: Resultsunclassified

See 1 more Smart Citation

Estudo das células Neuro2A sobre os biomateriais PCL e PLLA

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Toluidine blue has a high affinity for the carboxylic groups of proteins in nerve tissue. It has been widely employed in stains for myelin sheaths Bonner and Armati, 1991;Amilio et al, 1995;Aldini, et al, 1996;Lundborg and Kanje, 1996). In those reports, the myelin sheaths of myelinated fibers are stained dark blue by the toluidine blue.…”

Section: Toluidine Blue Stainmentioning

confidence: 99%

“…It is difficult to acquire donor nerves for grafting. Thus, considerable research has been conducted on peripheral nerve repair using the nerve bridge technique (Rosen et al, 1983Jenq and Coggeshall, 1984, 1987Seckel et al, 1984;Satou et al, 1986;Le Beau et al, 1988;Gibson and Daniloff, 1989;Hoppen et al, 1990;Aldini et al, 1996;Lundborg and Kanje, 1996). A nerve bridge technique is the introduction of the proximal and distal ends of a severed nerve into a tubular structure, which may or may not contain substances or other adjuncts for experimental purposes or to further promote healing.…”

Section: Statement Of the Problemmentioning

confidence: 99%

Development of a Multiple-Lumen Nerve Cuff Utilizing Growth Stimulant Patterns for Controlled Regeneration

et al. 1998

View full text Add to dashboard Cite

This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion.Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book.

show abstract

Poly(?-hydroxyacids) for application in the spinal cord: Resorbability and biocompatibility with adult rat Schwann cells and spinal cord

Gautier¹,

Oudega²,

Fragoso³

et al. 1998

J. Biomed. Mater. Res.

113

View full text Add to dashboard Cite

Future surgical strategies to restore neurological function in the damaged human spinal cord may involve replacement of nerve tissue with cultured Schwann cells using biodegradable guiding implants. We have studied the in vitro and in vivo degradability of various aliphatic polyesters as well as their effects on rat Schwann cells in vitro and on spinal cord tissue in vivo. In vitro, cylinders made of poly(D,L-lactic-co-glycolic acid) 50:50 (PLA25GA50) started to degrade at 7 days, compared with 28 days for cylinders made of poly(D,L-lactic acid) (PLA50). This faster degradation of PLA25GA50 was reflected by a much higher absorption of water. In vivo, after implantation of PLA25GA50 or PLA50 cylinders between the stumps of a completely transected adult rat spinal cord, the decrease in molecular weight of both polymers was similar to that found in vitro. In vitro degradation of poly(L-lactic acid) (PLA100) mixed with increasing amounts of PLA100 oligomers also was determined. The degradation rate of PLA100 mixed with 30% oligomers was found to be similar to that of PLA50. In vitro, PLA25GA50 and the breakdown products had no adverse effect on the morphology, survival, and proliferation of cultured rat Schwann cells. In vivo, PLA25GA50 cylinders were integrated into the spinal tissue 2 weeks after implantation, unlike PLA50 cylinders. At all time points after surgery, the glial and inflammatory response near the lesion site was largely similar in both experimental and control animals. At time points later than 1 week, neurofilament-positive fibers were found within PLA25GA50 cylinders or the remains thereof. Growth-associated protein 43, which is indicative of regenerating axons, was observed in fibers in the vicinity of the injury site and in the remains of PLA25GA50 cylinders. The results suggest that poly(alpha-hydroxyacids) are likely candidates for application in spinal cord regeneration paradigms involving Schwann cells.

show abstract

Effectiveness of a bioabsorbable conduit in the repair of peripheral nerves

Cited by 111 publications

References 13 publications

Estudo das células Neuro2A sobre os biomateriais PCL e PLLA

Estudo das células Neuro2A sobre os biomateriais PCL e PLLA

Development of a Multiple-Lumen Nerve Cuff Utilizing Growth Stimulant Patterns for Controlled Regeneration

Poly(?-hydroxyacids) for application in the spinal cord: Resorbability and biocompatibility with adult rat Schwann cells and spinal cord

Contact Info

Product

Resources

About