Effective production of biologically active water-soluble β-1,3-glucan by a coupled system of <i>Agrobacterium</i> sp. and <i>Trichoderma harzianum</i>

Liang, Ying; Zhu, Li; Gao, Minjie; Wu, Jianrong; Zhan, Xi

doi:10.1080/10826068.2018.1452259

Cited by 8 publications

(2 citation statements)

References 33 publications

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“…Most of the β-glucans produced on a commercial scale today are extracted from the cell walls of yeasts, fungi, and plants, although some are synthesized by fermentation (Zhu et al 2016 ; Liang et al 2018 ). The purified β-Gs are typically obtained by acidic hydrolysis steps, followed by selective precipitation using organic solvents (Shi 2016 ; Zhu et al 2016 ).…”

Section: β-Glucan Phosphorylases In β-Glucan Synthesismentioning

confidence: 99%

β-Glucan phosphorylases in carbohydrate synthesis

Ubiparip

Doncker

Beerens

et al. 2021

Appl Microbiol Biotechnol

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β-Glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of β-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of β-glucans as major bioactive compounds. Their preference for sugar phosphates (rather than nucleotide sugars) as donor substrates further underlines their significance for the carbohydrate industry. Presently, they are classified in the glycoside hydrolase families 94, 149, and 161 (www.cazy.org). Since the discovery of β-1,3-oligoglucan phosphorylase in 1963, several other specificities have been reported that differ in linkage type and/or degree of polymerization. Here, we present an overview of the progress that has been made in our understanding of β-glucan and associated β-glucobiose phosphorylases, with a special focus on their application in the synthesis of carbohydrates and related molecules. Key points • Discovery, characteristics, and applications of β-glucan phosphorylases. • β-Glucan phosphorylases in the production of functional carbohydrates.

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Section: β-Glucan Phosphorylases In β-Glucan Synthesismentioning

confidence: 99%

β-Glucan phosphorylases in carbohydrate synthesis

Ubiparip

Doncker

Beerens

et al. 2021

Appl Microbiol Biotechnol

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“…Microbial modification refers to the change for the composition and structure of the polysaccharide by the fermentation of microorganisms, thereby changing the physical properties and biological activity of the polysaccharide. Liang et al [80] developed an efficient and cost-effective microbial fermentation method for the direct production of water-soluble β-1,3-glucan (w-glucan), a coupled fermentation system of Agrobacterium sp. and Trichoderma harzianum (CFS-AT).…”

Section: Microbial Modificationmentioning

confidence: 99%

Effect of the Modifications on the Physicochemical and Biological Properties of β-Glucan—A Critical Review

Yuan

Lan

et al. 2019

Molecules

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β-Glucan exhibits many biological activities and functions such as stimulation of the immune system and anti-inflammatory, anti-microbial, anti-infective, anti-viral, anti-tumor, anti-oxidant, anti-coagulant, cholesterol-lowering, radio protective, and wound healing effects. It has a wide variety of uses in pharmaceutical, cosmetic, and chemical industries as well as in food processing units. However, due to its dense triple helix structure, formed by the interaction of polyhydroxy groups in the β-d-glucan molecule, it features poor solubility, which not only constrains its applications, but also inhibits its physiological function in vivo. One aim is to expand the applications for modified β-glucan with potential to prevent disease, various therapeutic purposes and as health-improving ingredients in functional foods and cosmetics. This review introduces the major modification methods required to understand the bioactivity of β-glucan and critically provides a literature survey on the structural features of this molecule and reported biological activity. We also discuss a new method to create novel opportunities to exploit maximally various properties of β-glucan, namely ultrasound-assisted enzymatic modification.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

Harvey

2021

Mass Spectrometry Reviews

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This review is the tenth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2018. Also included are papers that describe methods appropriate to glycan and glycoprotein analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, new methods, matrices, derivatization, MALDI imaging, fragmentation and the use of arrays. The second part of the review is devoted to applications to various structural types such as oligo‐ and poly‐saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Most of the applications are presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and highlights the impact that MALDI imaging is having across a range of diciplines. MALDI is still an ideal technique for carbohydrate analysis and advancements in the technique and the range of applications continue steady progress.

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Effective production of biologically active water-soluble β-1,3-glucan by a coupled system of Agrobacterium sp. and Trichoderma harzianum

Cited by 8 publications

References 33 publications

β-Glucan phosphorylases in carbohydrate synthesis

β-Glucan phosphorylases in carbohydrate synthesis

Effect of the Modifications on the Physicochemical and Biological Properties of β-Glucan—A Critical Review

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

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