Effective monitoring for ractopamine residues in samples of animal origin by SPR biosensor and mass spectrometry

Thompson, Colin S.; Haughey, Simon A.; Traynor, Imelda M.; Fodey, Terence L.; Elliott, Christopher T.; Antignac, Jean‐Philippe; Bizec, Bruno Le; Crooks, Steven R. H.

doi:10.1016/j.aca.2007.12.019

Cited by 48 publications

(29 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies of ractopamine residue detection after different treatment schedules in different animal species have been reported by several authors (Elliot et al, 1998;Qiang et al, 2007;Smith & Shelver, 2002;Thompson et al, 2008). Published studies report on tissue residues of ractopamine and its urinary excretion (Antignac et al, 2002;Blanca et al, 2005;Dickson et al, 2005;Moragues & Igualada, 2009;Nielen et al, 2008;Thompson et al, 2008;Van Hoof et al, 2005) and ractopamine residues in hair (Nielen et al, 2008). However, there are little data on the accumulation of ractopamine in pig hair (white or black) as a novel matrix for the control of ractopamine illegal use.…”

Section: Applicability Of Study Resultsmentioning

confidence: 99%

“…There are literature reports on ractopamine determination using different techniques (Shelver & Smith, 2003;Smith et al, 1993;Thompson et al, 2008;Turberg et al, 1995). Studies suggest the use of LC/MS/MS systems as probably the best methods to improve sensitivity in determination of -adrenergic agonists, while retaining excellent selectivity (Smith & Shelver, 2002), pointing to UPLC-MS/MS as one of the most efficient methods.…”

Section: Applicability Of Study Resultsmentioning

confidence: 99%

“…Studies carried out in cattle and sheep report on detectable ractopamine residues in urine 5 to 7 days after the last exposure to dietary ractopamine, pointing that hydrolysis of ractopamine metabolites may extend the period in which it is detected in cattle (Smith & Shelver, 2002). In their study, Thompson et al (2008) fed pigs a feed containing 18 mg/kg ractopamine once daily for 10 days (180 mg of ractopamine in total). Ractopamine residues in pig urine were detectable by both screening and confirmatory methods until day 21 of treatment withdrawal.…”

Section: Concentration Of Ractopamine Residues 331 Urine Residuesmentioning

confidence: 99%

See 2 more Smart Citations

Determination of Ractopamine Residues in Pigs by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

Pleadin¹,

Vulić²,

Perši³

et al. 2012

Tandem Mass Spectrometry - Applications and Principles

View full text Add to dashboard Cite

Section: Applicability Of Study Resultsmentioning

confidence: 99%

Section: Applicability Of Study Resultsmentioning

confidence: 99%

Section: Concentration Of Ractopamine Residues 331 Urine Residuesmentioning

confidence: 99%

See 1 more Smart Citation

Determination of Ractopamine Residues in Pigs by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

Pleadin¹,

Vulić²,

Perši³

et al. 2012

Tandem Mass Spectrometry - Applications and Principles

View full text Add to dashboard Cite

“…It is licensed for use as an animal growth promoter in some countries e.g. USA and Canada, but is either unlicensed or banned by many other countries [1]. Meat products obtained from treated animals pose a potential risk for consumer health, mainly to persons with asthmatic or cardiac complaints [2].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric, UV–Vis Spectrometric and Fluorescence Study of the Interaction of Ractopamine and DNA with the Aid of Multivariate Curve Resolution‐Alternating Least Squares

Wang

Kokot

2010

Electroanalysis

View full text Add to dashboard Cite

Interaction of a steroid drug, Ractopamine (RAC), and DNA was investigated by electroanalysis-differential pulse and cyclic voltammetry (DPV and CV), and UV-vis and fluorescence spectroscopy. DPV showed that RAC intercalated with DNA, and CV indicated that the reaction mechanism of RAC and dsDNA involved irreversible oxidation with the loss of two H þ and a transfer of two electrons. Reaction binding parameters were obtained. Pure spectra of RAC, DNA and the RAC-DNA complex, and their concentrations were extracted by multivariate curve resolution-alternating least squares method (MCR-ALS). Concentration profiles indicated quantitatively the course of the reaction.

show abstract

“…In a more recent publication Thompson et al (2008) described a method based on SPR biosensor for the monitoring of ractopamine residues in urine and liver. The LODs for urine and liver were quoted as 0.34 µg/l and 0.19 µg/kg, respectively.…”

Section: Screening Methodsmentioning

confidence: 99%

Safety evaluation of ractopamine

Authority¹

2009

EFS2

View full text Add to dashboard Cite

SUMMARYRactopamine hydrochloride is pharmacologically classified as a phenethanolamine β-adrenoceptor agonist. The use of the substance as a feed additive is authorised in different countries (USA, Canada, Japan and Mexico) for growth promotion of fattening pigs and cattle. Ractopamine has not been assessed in the EU so far.Following a request from the European Commission, the European Food Safety Authority (EFSA) was asked to provide an opinion on the JECFA evaluation for ractopamine hydrochloride, having consulted and closely co-operated with other organisations such as EMEA and the Community Reference Laboratory responsible for β-agonists (BVL in Berlin).The metabolic fate of ractopamine hydrochloride is similar in the target species (pig and cattle), laboratory animals and humans.The FEEDAP Panel concluded from an acute study in dogs that tachycardia and peripheral vasodilatation observed are in line with the expected pharmacological action. From another acute study in dogs, with limited statistical power, a pharmacological NOAEL of 2 µg kg -1 bw could be derived.Comparing dog and monkey data it appeared that the dog could be considered as more sensitive to ractopamine (β-adrenergic substances). However, the FEEDAP Panel considered that there was not enough data to support this conclusion.NOAEL's derived from pharmacological repeated dose studies should not be regarded as a meaningful basis for an ADI because of the observed down regulation of lung β-adrenergic Opinion on safety evaluation of ractopamineThe EFSA Journal (2009) 1041, 2-52 receptors, at least as long as dose-and time-dependency and β-adrenoceptors speciation is not established. When evaluating hypothetical risks for the consumer, data from acute pharmacological studies would better reflect the consumer situation after intake of a single meal containing ractopamine residues.The NOAELs derived from toxicological end points were considerably higher than those from pharmacological end points. Effects observed in toxicity studies were mostly related to the pharmacological action.Although a series of mutation studies in prokaryotes and eukaryotic systems were negative, several in vitro tests were positive. The FEEDAP Panel considered that some positive genotoxicity studies in vitro are a possible cause of concern. However, these results have to be considered in conjunction with the carcinogenicity studies provided.The FEEDAP Panel concluded that all treatment-related effects observed in the long-term studies in mice and rats were attributable to the β-adrenergic activity of ractopamine. It shares the JECFA and FDA opinion, considering that the induction of leiomyomas is a non-genotoxic event with a threshold and ractopamine is not a direct carcinogen. Considering all studies, the FEEDAP Panel concluded that ractopamine is not mutagenic and is not likely to present a carcinogenic risk to consumers.Since data in laboratory animals gave a wide range of NOAELs, the available human data was considered pivotal by JECFA as it was by the FEEDAP Panel when ...

show abstract

Effective monitoring for ractopamine residues in samples of animal origin by SPR biosensor and mass spectrometry

Cited by 48 publications

References 18 publications

Determination of Ractopamine Residues in Pigs by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

Determination of Ractopamine Residues in Pigs by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

Voltammetric, UV–Vis Spectrometric and Fluorescence Study of the Interaction of Ractopamine and DNA with the Aid of Multivariate Curve Resolution‐Alternating Least Squares

Safety evaluation of ractopamine

Contact Info

Product

Resources

About