Effective Isolation for Lung Carcinoma Cells Based on Immunomagnetic Separation in a Microfluidic Channel

Abstract

In this paper, we developed an isolation system for A549 human lung carcinoma cells as an effective factor for the early diagnosis of lung cancer. A microfluidic immunomagnetic method was used, in which the combination of immunomagnetic separation and a microfluidic system allowed for increased isolation efficiency with uncomplicated manipulation. In the microfluidic immunomagnetic strategy, A549 cells were combined with aptamer-conjugated carboxylated magnetic beads and then collected in a specified region by applying a magnetic field. The results were recorded using a fluorescence microscope, and the captured targets were then quantified. The isolation efficiency of A549 cells is up to 77.8%. This paper developed a simple working procedure, which is less time consuming, high-throughput, and trustworthy for the isolation of A549 cells. This procedure can be a useful reference method for the development of an effective diagnosis and treatment method for lung cancer in the future.

Toward Personalized Nanomedicine: The Critical Evaluation of Micro and Nanodevices for Cancer Biomarker Analysis in Liquid Biopsy

“…The released cells can then be recaptured after engaging the rod into the plastic sheath, and this cycle can be repeated to eliminate undesired background cells. [31] Details of [26] Copyright 2021, MDPI. Reproduced with permission.…”

“…[26] Aptamer-based sensing has benefits over antibodies such as a short production period, large-scale production convenience, batch-to-batch stability (under differing pH, temperature, and ionic conditions), lower cost, low toxicity, and storage ease, among others. [26,33] Endless aptamer sequences can also be selected from combinatorial libraries of synthetic nucleic acids using the systematic evolution of ligands (SELEX) process. [34] An aptamer-based immunomagnetic platform was created by Vu-Dinh, et al (Figure 2c) [26] to overcome antibodybased sensing limitations.…”

“…[26,33] Endless aptamer sequences can also be selected from combinatorial libraries of synthetic nucleic acids using the systematic evolution of ligands (SELEX) process. [34] An aptamer-based immunomagnetic platform was created by Vu-Dinh, et al (Figure 2c) [26] to overcome antibodybased sensing limitations. This platform targets cells using an aptamer specific to the A549 cell via aptamer-conjugated carboxylated magnetic beads, with cell staining and fluorescence microscopy detection.…”

“…Aptamer-based sensing (as previously discussed within the immune magnetic platforms section) has great potential to overcome these bottlenecks for microfluidic and electrochemical POC devices, as aptamers have high levels of stability under differing pH, temperature, and ionic conditions. [26,33] Besides the challenges associated with the stability and storage conditions, the largest challenge hindering POC platforms from entering the clinical setting is the requirement for specialized and often bulky equipment such as microscopes, pumps, heating equipment, centrifugation, mixing equipment, etc. Fluorescence microscopy is an essential component in many biomedical applications as well as many emerging liquid biopsy technologies.…”

“…c) An aptamer-based immunomagnetic platform for the isolation of human lung carcinoma cells and d) 3D printed immunomagnetic concentrator (3DPIC) for the luminescent detection of circulating tumor cells. Reproduced with permission [26]. Copyright 2021, MDPI.…”