Immunomagnetic separation in a novel cavity-added serpentine microchannel structure for the selective isolation of lung adenocarcinoma cells

Vu-Dinh, Hien; Quang, Loc Do; Chang, Chung‐Liang; Nhu, Cuong Nguyen; Thanh, Hang Tran; Bui, Tung Thanh; Duc, Trinh Chu; Jen, Chun-Ping

doi:10.1007/s10544-021-00589-6

Cited by 7 publications

(8 citation statements)

References 52 publications

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“…The governing equations represent the forces used, as mentioned in the previous section (Theory). In addition, the calculation of Brownian force incorporated the equations recommended by the software, as referenced in a previous parallel study [21]. Gravity was neglected due to the substantial ow rate and small channel size, which signi cantly diminished the role of this force in the overall calculation.…”

Section: Simulation Results Of the Cell Isolation E Ciencymentioning

confidence: 99%

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The Effect of Magnetic Bead Size on the Isolation Efficiency of Lung Cancer Cells in a Serpentine Microchannel with Added Cavities

Su,

Vu-Dinh,

Lin

et al. 2023

Preprint

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An investigation was conducted to examine the effect of magnetic bead (MB) size on the effectiveness of isolating lung cancer cells using the immunomagnetic separation (IMS) method in a serpentine microchannel with added cavities (SMAC) structure. Carboxylated-magnetic beads were specifically attached to cells through a modification process using aptamers. Cells immobilized with different sizes (in micrometers) of MBs were captured and isolated in the proposed device for comparison and analysis. A computational model was performed to clarify the working principles of the device. To accurately evaluate the impact of MB sizes on capture efficiency, we have included the issue of MB-cell accumulation at the interface between the vertical inlet and the microfluidic channel, despite it being overlooked in many previous studies. A detailed examination of both the accumulation rate and the capture efficiency was conducted. Our findings demonstrated an increasing trend in binding efficiency as the size of MBs decreased, evidenced by coverage values of 50.5%, 60.1%, and 73.4% for MB sizes of 1.36 μm, 3.00 μm, and 4.50 μm, respectively. Furthermore, the overall capture efficiency (without considering the inlet accumulation) was higher for smaller MBs. However, when accounting for the actual number of cells entering the channel (i.e., the effective capture), larger MBs showed higher capture efficiency. The highest effective capture achieved was 88.4% for the size of 4.50 μm. This research provides a comprehensive insight into the impact of MB size on the performance of IMS-based devices and holds promise for the efficient separation of circulating cancer cells in practical applications.

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Section: Simulation Results Of the Cell Isolation E Ciencymentioning

confidence: 99%

“…Finally, the PDMS channel was peeled off and punched at the inlets and outlets for plasma bonding to the glass substrate. Details of the fabrication process can be found in our previous work [21].…”

Section: Micro Uidic Device Design and Fabricationmentioning

confidence: 99%

The Effect of Magnetic Bead Size on the Isolation Efficiency of Lung Cancer Cells in a Serpentine Microchannel with Added Cavities

Su,

Vu-Dinh,

Lin

et al. 2023

Preprint

Self Cite

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“…Jiang et al [279] presented a chitosan scaffold with controllable geometric microchannels using the freezedrying method (figure 15(d)), demonstrating that functional microfluidic devices can promote cell infiltration and distribution as well as tissue ingrowth. Vu-dinh et al [280] proposed a novel microfluidic device specifically for isolating human lung carcinoma in microchannels (figure 15(e)). By exploiting magnetic nanoparticles, the target A549 cells immobilized by magnetic beads can be manipulated by an external magnetic field and trapped in cavities, demonstrating excellent cell separation performance.…”

Section: Biomedical Fieldmentioning

confidence: 99%

“…Reprinted from [279], © 2021 Elsevier B.V. All rights reserved. (e) Lung adenocarcinoma cells selection by cavity-added serpentine microchannels [280]. Reproduced from [280], with permission from Springer Nature.…”

Section: Capillaric Circuitsmentioning

confidence: 99%

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Functional microfluidics: theory, microfabrication, and applications

Xie,

Zhan,

et al. 2024

Int. J. Extrem. Manuf.

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Microfluidic devices are composed of microchannels with a diameter ranging in ten to a few hundred micrometers. Thus, quite small (10-9 to 10-18 litres) amount of liquid can be manipulated by such a precise system. In the past three decades, significant progresses in materials, microfabrication, and various applications have boosted the development of promising functional microfluidic devices. In this review, the recent progresses on novel microfluidic devices with various functions and applications are presented. First, the theory and numerical methods for studying the performance of microfluidic devices are briefly introduced. Then, materials, and fabrication methods of functional microfluidic devices are summarized. Next, from the viewpoint of the applications of the microfluidic devices, the recent significant advances in heat sink, clean water production, chemical reactions, sensor, biomedical field, capillaric circuits, flexible and wearable electronic devices, microrobotics, etc., in turns are then highlighted. Finally, personal perspectives on the challenges and future developments of functional microfluidic devices, aiming to motivate researchers from the fields of engineering, materials, chemistry, mathematics, physics, etc. working together to promote further development and applications of functional microfluidic devices, for the specific purpose of carbon neutrality are provided.

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Enhancing the efficiency of lung cancer cell capture using microfluidic dielectrophoresis and aptamer‐based surface modification

Lin,

Su,

Huang

et al. 2024

Electrophoresis

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Metastasis remains a significant cause to cancer‐related mortality, underscoring the critical need for early detection and analysis of circulating tumor cells (CTCs). This study presents a novel microfluidic chip designed to efficiently capture A549 lung cancer cells by combining dielectrophoresis (DEP) and aptamer‐based binding, thereby enhancing capture efficiency and specificity. The microchip features interdigitated electrodes made of indium‐tin‐oxide that generate a nonuniform electric field to manipulate CTCs. Following three chip design, scenarios were investigated: (A) bare glass surface, (B) glass modified with gold nanoparticles (AuNPs) only, and (C) glass modified with both AuNPs and aptamers. Experimental results demonstrate that AuNPs significantly enhance capture efficiency under DEP, with scenarios (B) and (C) exhibiting similar performance. Notably, scenario (C) stands out as aptamer‐functionalized surfaces resisting fluid shear forces, achieving CTCs retention even after electric field deactivation. Additionally, an innovative reverse pumping method mitigates inlet clogging, enhancing experimental efficiency. This research offers valuable insights into optimizing surface modifications and understanding key factors influencing cell capture, contributing to the development of efficient cell manipulation techniques with potential applications in cancer research and personalized treatment options.

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Immunomagnetic separation in a novel cavity-added serpentine microchannel structure for the selective isolation of lung adenocarcinoma cells

Cited by 7 publications

References 52 publications

The Effect of Magnetic Bead Size on the Isolation Efficiency of Lung Cancer Cells in a Serpentine Microchannel with Added Cavities

The Effect of Magnetic Bead Size on the Isolation Efficiency of Lung Cancer Cells in a Serpentine Microchannel with Added Cavities

Functional microfluidics: theory, microfabrication, and applications

Enhancing the efficiency of lung cancer cell capture using microfluidic dielectrophoresis and aptamer‐based surface modification

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