Effective co-delivery of nutlin-3a and p53 genes via core–shell microparticles for disruption of MDM2–p53 interaction and reactivation of p53 in hepatocellular carcinoma

Davoodi, Pooya; Madapusi, Srinivasan; Wang, Chi-Hwa

doi:10.1039/c7tb00481h

Cited by 20 publications

(10 citation statements)

References 56 publications

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“…Another valuable advantage of electrohydrodynamic techniques is how they enable the encapsulation of bioactive elements with distinct chemical structures, including not only small molecules but also DNA [ 290 , 291 ] and proteins [ 285 , 292 , 293 ]. Cell electrospinning has also been performed, allowing the direct incorporation of live cells during the scaffold manufacturing process [ 294 ].…”

Section: Osteochondral Tissue Engineeringmentioning

confidence: 99%

“…Importantly, all scaffolds supported hBMSC attachment and proliferation, as well as ECM production and expression of chondrogenic genes (type II collagen, Sox9, aggrecan, and proteoglycan 4 (PRG4)), particularly KGN-loaded fibres [289]. Another valuable advantage of electrohydrodynamic techniques is how they enable the encapsulation of bioactive elements with distinct chemical structures, including not only small molecules but also DNA [290,291] and proteins [285,292,293]. Cell electrospinning has also been performed, allowing the direct incorporation of live cells during the scaffold manufacturing process [294].…”

Section: Electrospinningmentioning

confidence: 99%

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Osteochondral Tissue Engineering: The Potential of Electrospinning and Additive Manufacturing

Gonçalves¹,

Moreira²,

Weber

et al. 2021

Pharmaceutics

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The socioeconomic impact of osteochondral (OC) damage has been increasing steadily over time in the global population, and the promise of tissue engineering in generating biomimetic tissues replicating the physiological OC environment and architecture has been falling short of its projected potential. The most recent advances in OC tissue engineering are summarised in this work, with a focus on electrospun and 3D printed biomaterials combined with stem cells and biochemical stimuli, to identify what is causing this pitfall between the bench and the patients’ bedside. Even though significant progress has been achieved in electrospinning, 3D-(bio)printing, and induced pluripotent stem cell (iPSC) technologies, it is still challenging to artificially emulate the OC interface and achieve complete regeneration of bone and cartilage tissues. Their intricate architecture and the need for tight spatiotemporal control of cellular and biochemical cues hinder the attainment of long-term functional integration of tissue-engineered constructs. Moreover, this complexity and the high variability in experimental conditions used in different studies undermine the scalability and reproducibility of prospective regenerative medicine solutions. It is clear that further development of standardised, integrative, and economically viable methods regarding scaffold production, cell selection, and additional biochemical and biomechanical stimulation is likely to be the key to accelerate the clinical translation and fill the gap in OC treatment.

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Section: Osteochondral Tissue Engineeringmentioning

confidence: 99%

Section: Electrospinningmentioning

confidence: 99%

Osteochondral Tissue Engineering: The Potential of Electrospinning and Additive Manufacturing

Gonçalves¹,

Moreira²,

Weber

et al. 2021

Pharmaceutics

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“…Additionally, nutlin-3a induced excessive oxidative stress in cancer cells. Moreover, a secondary apoptotic pathway by nutlin-3a was induced by the overproduction of ROS with irreversible destructive effects on subcellular organelles such as the nucleus and mitochondria [261]. Wang et al also used core–shell-structured polyplex to transfer the fluorescent probe YOYO-1-labeled DNA with high transfection efficacy and low toxicity.…”

Section: Gene Deliverymentioning

confidence: 99%

Nanocomposites as biomolecules delivery agents in nanomedicine

Bamburowicz-Klimkowska

Popławska

Grudziński

2019

J Nanobiotechnol

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Nanoparticles (NPs) are atomic clusters of crystalline or amorphous structure that possess unique physical and chemical properties associated with a size range of between 1 and 100 nm. Their nano-sized dimensions, which are in the same range as those of vital biomolecules, such as antibodies, membrane receptors, nucleic acids, and proteins, allow them to interact with different structures within living organisms. Because of these features, numerous nanoparticles are used in medicine as delivery agents for biomolecules. However, off-target drug delivery can cause serious side effects to normal tissues and organs. Considering this issue, it is essential to develop bioengineering strategies to significantly reduce systemic toxicity and improve therapeutic effect. In contrast to passive delivery, nanosystems enable to obtain enhanced therapeutic efficacy, decrease the possibility of drug resistance, and reduce side effects of “conventional” therapy in cancers. The present review provides an overview of the most recent (mostly last 3 years) achievements related to different biomolecules used to enable targeting capabilities of highly diverse nanoparticles. These include monoclonal antibodies, receptor-specific peptides or proteins, deoxyribonucleic acids, ribonucleic acids, [DNA/RNA] aptamers, and small molecules such as folates, and even vitamins or carbohydrates.

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“…In addition, in some cases, it has been reported that they are able to offer excellent protection against enzymatic degradation or immune attack [28][29][30]. Finally, NPs may contain factors able to down-regulate oncogenes or restore tumor suppressor genes [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Nanotechnology for the Treatment of Melanoma

et al. 2021

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Melanoma is one of the most aggressive forms of skin cancer, with few possibilities for therapeutic approaches, due to its multi-drug resistance and, consequently, low survival rate for patients. Conventional therapies for treatment melanoma include radiotherapy, chemotherapy, targeted therapy, and immunotherapy, which have various side effects. For this reason, in recent years, pharmaceutical and biomedical research has focused on new sito-specific alternative therapeutic strategies. In this regard, nanotechnology offers numerous benefits which could improve the life expectancy of melanoma patients with very low adverse effects. This review aims to examine the latest advances in nanotechnology as an innovative strategy for treating melanoma. In particular, the use of different types of nanoparticles, such as vesicles, polymers, metal-based, carbon nanotubes, dendrimers, solid lipid, microneedles, and their combination with immunotherapies and vaccines will be discussed.

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Effective co-delivery of nutlin-3a and p53 genes via core–shell microparticles for disruption of MDM2–p53 interaction and reactivation of p53 in hepatocellular carcinoma

Abstract: Using core–shell microparticles encapsulating p53/β-CD-g-CS NPs and nutlin-3a effectively suppressed p53–MDM2 interaction and restored p53 apoptotic activity in cancer cells.

Cited by 20 publications

References 56 publications

Osteochondral Tissue Engineering: The Potential of Electrospinning and Additive Manufacturing

Osteochondral Tissue Engineering: The Potential of Electrospinning and Additive Manufacturing

Nanocomposites as biomolecules delivery agents in nanomedicine

Recent Advances in Nanotechnology for the Treatment of Melanoma

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