Effective Cell Harvesting of the Halotolerant Microalga Dunaliella tertiolecta with pH Control

Horiuchi, Jun‐ichi; Ohba, Ichigaku; Tada, Kiyoshi; KOBAYASHI, MASAYOSHI; Kanno, Tohru; Kishimoto, Michimasa

doi:10.1263/jbb.95.412

Cited by 21 publications

(27 citation statements)

References 5 publications

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“…In this study, among all D. salina cultures, the high growth rate was observed in cultures aerated with either 3.0 or 9.0% CO 2 . These findings results are consistent with that results reported in several reports, that the D. tertiolecta could be grown well in the cultures aerated with CO 2 ranges from 2 to 10% [24,25]. Cheng et al [26] reported that the high biomass of Chlorella vulgaris was obtained in cultures aerated with 2% CO 2 level.…”

Section: Growth Of D Salina Aerated With Different Co 2 Levelssupporting

confidence: 92%

Lipid Induction in Dunaliella salina Culture Aerated with Various Levels CO2 and Its Biodiesel Production

Bouaid¹

2014

J Aquac Res Development

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Section: Growth Of D Salina Aerated With Different Co 2 Levelssupporting

confidence: 92%

Lipid Induction in Dunaliella salina Culture Aerated with Various Levels CO2 and Its Biodiesel Production

Bouaid¹

2014

J Aquac Res Development

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“…In consequence, sedimentation alone is largely dismissed as a viable harvesting method. However, flocculation caused by alkaline adjustment has been used to effectively remove Dunalliella testolata (Horiuchi et al 2003) and Chaetoceros sp. from fluids (Csordas & Wang 2004;Knuckey et al 2006).…”

Section: Cell Harvestingmentioning

confidence: 99%

Placing microalgae on the biofuels priority list: a review of the technological challenges

Greenwell

Laurens

Shields

et al. 2009

J. R. Soc. Interface.

714

359

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Microalgae provide various potential advantages for biofuel production when compared with 'traditional' crops. Specifically, large-scale microalgal culture need not compete for arable land, while in theory their productivity is greater. In consequence, there has been resurgence in interest and a proliferation of algae fuel projects. However, while on a theoretical basis, microalgae may produce between 10-and 100-fold more oil per acre, such capacities have not been validated on a commercial scale. We critically review current designs of algal culture facilities, including photobioreactors and open ponds, with regards to photosynthetic productivity and associated biomass and oil production and include an analysis of alternative approaches using models, balancing space needs, productivity and biomass concentrations, together with nutrient requirements. In the light of the current interest in synthetic genomics and genetic modifications, we also evaluate the options for potential metabolic engineering of the lipid biosynthesis pathways of microalgae. We conclude that although significant literature exists on microalgal growth and biochemistry, significantly more work needs to be undertaken to understand and potentially manipulate algal lipid metabolism. Furthermore, with regards to chemical upgrading of algal lipids and biomass, we describe alternative fuel synthesis routes, and discuss and evaluate the application of catalysts traditionally used for plant oils. Simulations that incorporate financial elements, along with fluid dynamics and algae growth models, are likely to be increasingly useful for predicting reactor design efficiency and life cycle analysis to determine the viability of the various options for largescale culture. The greatest potential for cost reduction and increased yields most probably lies within closed or hybrid closed -open production systems.

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“…Este resultado se debió a una disminución en el potencial zeta por aumento del pH, lo que produce la neutralización de la carga superficial de las células y por consecuencia su floculación (Wu et al, 2012;Shen et al, 2013). Resultados similares fueron reportados por Horiuchi et al (2003) con Dunaliella tertiolecta. Estos autores obtuvieron una rápida floculación al adicionar NaOH al medio de cultivo, entre valores de pH 8,6 y 10,5 e indicaron una recuperación de la biomasa celular mayor al 90%.…”

Section: Discussionunclassified

“…Sin embargo, se ha demostrado que con la floculación producida por agentes alcalinos (e.g., Ca(OH)2, Mg(OH)2 y NaOH), aplicada en la producción de concentrados microalgales, se obtiene mayor eficiencia, menor costo y garantiza la inocuidad (Folkman & Wachs, 1973;Horiuchi et al, 2003;Schlesinger et al, 2012;Besson & Guiraud, 2013). Además, el alto pH de este método reduce eficazmente la carga bacteriana de la biomasa de microalgas y del agua del proceso (Huo et al, 2014).…”

Section: Introductionunclassified

Floculacion de Nannochloropsis sp. inducida por hidroxido de sodio: eficiencia de floculacion, efecto sobre la viabilidad microalgal y su uso como alimento para rotiferos

Rojo-Cebreros

Morales-Plascencia²,

Ibarra‐Castro

et al. 2016

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RESUMEN.Los laboratorios acuícolas altamente dependientes de Nannochloropsis sp. desarrollan mecanismos para su producción masiva, con el desafío de poder extraer altas concentraciones celulares. La floculación por agentes alcalinos es una alternativa que promete mayores ventajas, y el hidróxido de sodio puede ser el agente floculante más conveniente. Sin embargo, para lograr una eficiencia máxima de floculación son determinantes la cantidad del agente floculante, tiempo de exposición, concentración celular y tipo de microalga. Por lo anterior, se determinó la cantidad de NaOH 0,5N y el tiempo de exposición necesarios para lograr un pH óptimo de floculación en cosechas de Nannochloropsis sp. con concentraciones celulares mayores a 50x10 6 cél mL -1. Los resultados mostraron que con 17 mL de NaOH 0,5N por litro de cultivo de microalgas se puede lograr una eficiencia de floculación de 94,9% a pH 9,7 en menos de 60 min. Las células del sobrenadante (subproducto del proceso) fueron viables como inóculo de un nuevo cultivo de microalgas. El concentrado microalgal (355,7x10 6 cél mL ) obtenido fue adecuado como alimento para rotíferos. Por lo tanto, se recomienda el empleo de NaOH 0,5N como método simple, práctico y de bajo costo para producir concentrados de Nannochloropsis sp. con fines acuícolas. Palabras clave: Nannochloropsis sp., floculación alcalina, microalgas, acuicultura.Flocculation of Nannochloropsis sp. induced by sodium hydroxide: flocculation efficiency, viability effect on microalgae and their use as food for rotifers ABSTRACT. Aquaculture laboratories highly dependent of Nannochloropsis sp. develop mechanisms for mass production, with the challenge of be able to extract high cell concentrations from the culture medium. Flocculation by alkaline agents is an alternative that promises greater advantages, and sodium hydroxide may be the most convenient. However, to achieve maximum efficiency of flocculation are important the amount of flocculent, exposure time, cell concentration and type of microalgae. Therefore, the amount of 0.5N NaOH and the exposure time required are investigated to Nannochloropsis sp., harvests at high density at 50x10 6 cells mL ) produced was adequate as food for rotifers. Therefore, employment of 0.5N NaOH is recommended as a simple, convenient and inexpensive method for producing Nannochloropsis sp. concentrates for aquaculture purposes.

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Effective Cell Harvesting of the Halotolerant Microalga Dunaliella tertiolecta with pH Control

Cited by 21 publications

References 5 publications

Lipid Induction in Dunaliella salina Culture Aerated with Various Levels CO2 and Its Biodiesel Production

Lipid Induction in Dunaliella salina Culture Aerated with Various Levels CO2 and Its Biodiesel Production

Placing microalgae on the biofuels priority list: a review of the technological challenges

Floculacion de Nannochloropsis sp. inducida por hidroxido de sodio: eficiencia de floculacion, efecto sobre la viabilidad microalgal y su uso como alimento para rotiferos

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