Effective bioprinting resolution in tissue model fabrication

Miri, Amir K.; Mirzaee, Iman; Hassan, Shabir; Oskui, Shirin Mesbah; Nieto, Daniel; Khademhosseini, Ali; Zhang, Yu Shrike

doi:10.1039/c8lc01037d

Cited by 167 publications

(133 citation statements)

References 102 publications

(138 reference statements)

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“…In fact, in many bioprinting systems, a thin layer of coating material such as 3-(Trimethoxysilyl)propyl methacrylate (TMSPMA) is usually used to coat the substrate in order to enhance the hydrophobicity before printing. 61,62 This coating will increase the contact angle and the ultimate contact angle is typically in the range of 45 ○ ≤ θe ≤ 135 ○ . Therefore, our equilibrium contact angle of 60 ○ is an accepted value.…”

Section: Problem Statement and Formulationmentioning

confidence: 99%

A computational study of droplet-based bioprinting: Effects of viscoelasticity

2019

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Section: Problem Statement and Formulationmentioning

confidence: 99%

A computational study of droplet-based bioprinting: Effects of viscoelasticity

2019

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“…Indeed, subcellular features are emerging as key markers for disease progression, such as multinucleation, sarcomere and T-tubule density and alignment, capillary density and alignment, and canaliculi dilation 27,[40][41][42][43][44][45][46][47] . In addition, recent tissue engineering approaches demonstrated cells and extracellular matrix can be bioprinted with micron-level resolution 48,49 , the datasets generated by this method will also be useful blueprints for engineering human tissues.…”

Section: Discussionmentioning

confidence: 99%

High-Resolution 3D Fluorescent Imaging of Intact Tissues

El‐Nachef

Yang

et al. 2019

Preprint

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Histological analysis of fluorescently labeled tissues has been a critical tool to understand molecular organization in situ. However, assessing molecular structures within large cells and in the context of human organ anatomy has been challenging because it requires penetration of staining reagents and light deep into opaque tissues, while also conforming to the spatial constraints of high-resolution objective lenses. This methodology article describes optimized sample preparation for sub-micron resolution 3D imaging in human and rodent tissues, yielding imaging depth (>100 µm) and resolution (<0.012 µm 3 voxel size) that has previously been limited to whole-mount in vitro organoid systems, embryos, and small model organisms. Confocal images of adult human and rodent organs, including heart, kidney, and liver, were generated for several chemical and antibody stains in cleared tissue sections >100 µm thick. This method can be readily adopted by any lab performing routine histology and takes 3 days from the start of tissue preparation to 3D images. Contributions D.E. designed the study, performed experiments, analyzed results, and wrote the manuscript. A.M.M. performed cardiac cell therapy surgeries and animal care. X.Y. generated PSC-derived cardiac myocytes for cell therapy. W.R.M., C.E.M., and X.Y. edited the manuscript. W.R.M. and C.E.M. provided supervision and acquired funding. Competing Interests W.R.M. and C.E.M. are scientific founders and equity holders in Cytocardia. The other authors declare no competing interests.

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“…Still, a major challenge in the field is the limited resolution of the approach which does not enable the production of vascular constructs on a small scale. Indeed, the best resolution available for arteriole/venule structures and for capillaries is 30 µm and 8 µm, respectively [16].…”

Section: Tissue Engineering and Drug Deliverymentioning

confidence: 99%