Effective and Preparative Separation of Bioactive Flavonoids From<i>Gynostemma Pentaphyllum</i>Tea Using Elution-Extrusion Counter-Current Chromatography

Wu, Jianbing; Chen, Zhe; Zhang, Chuanlan; Lu, Yanbin; Wang, Huizhong

doi:10.1080/01496395.2012.710887

Cited by 6 publications

(7 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The E1 products of gypenosides LVI and XLVI, the damulins A (15) and B (16) (C-2-OH) and the C-3-OH isomers, damulins E (17) and F (18), exhibited low to moderate monolayer permeability with P app values ranging from 1.33 (±0.073) to 6.2 (±0.248) × 10 −6 cm/s. Damulins A and B (15 and 16) differ only in the position of the double bond at C-20 of the side chain, with 15 being the Δ 20,22 isomer and 16 the less stable terminal double-bond isomer (Δ 20,21 ). Damulins E and F (17 and 18) share the same structural relationship (Figure 1).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Reports suggest that approximately 165 saponins are present in G. pentaphyllum . 18 , 21 The major saponins present in G. pentaphyllum belong to the dammarane class of saponins (known as gypenosides, if isolated from G. pentaphyllum ). Dammarane saponins are characterized by a dammarane aglycone with an acyclic side chain ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

“…Dammarane aglycones are tetracyclic triterpenoid sapogenins in which C-1, C-2, C-3, C-12, and C-20 positions are either hydroxylated or glycosylated and the C-17 position can possess a variety of modified side chains. A variety of flavonoids have also been isolated from G. pentaphyllum with the free aglycone forms (quercetin, kaempferol, and isorhamnetin) being reported; 21 , 22 however, the most prominent flavonoids present in G. pentaphyllum are found as quercetin and kaempferol glycosides ( Figure 2 ). 22 …”

Section: Introductionmentioning

confidence: 99%

“…Reports suggest that approximately 165 saponins are present in G. pentaphyllum. 18,21 The major saponins present in G. pentaphyllum belong to the dammarane class of saponins (known as gypenosides, if isolated from G. A variety of flavonoids have also been isolated from G. pentaphyllum with the free aglycone forms (quercetin, kaempferol, and isorhamnetin) being reported; 21,22 however, the most prominent flavonoids present in G. pentaphyllum are found as quercetin and kaempferol glycosides (Figure 2). 22 A large number of studies have already established the diverse pharmacological benefits of organic or aqueous crude extracts of G. pentaphyllum or of a saponin-rich extract preparation.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Reports suggest that approximately 165 saponins are present in G. pentaphyllum. , The major saponins present in G. pentaphyllum belong to the dammarane class of saponins (known as gypenosides, if isolated from G.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Caco-2 Cell Permeability of Flavonoids and Saponins from Gynostemma pentaphyllum: the Immortal Herb

Ahmed

Leach²,

Wohlmuth

et al. 2020

ACS Omega

View full text Add to dashboard Cite

Gynostemma pentaphyllum (the immortal herb) has been an important component of Chinese Traditional Medicine for millennia. Recent clinical studies have revealed that the plant exhibits numerous beneficial biological activities, making it of interest to the pharmaceutical industry. An extract of the herb contains over 200 individual secondary metabolites including flavonol glycosides and dammarane saponins. To focus attention on the compounds most likely to be responsible for the biological activities, this study predicts the potential oral bioavailability of nine dammarane saponins and five flavonol glycosides from G. pentaphyllum using the Caco-2 cell monolayer permeability model. Two flavonoids, 8 and 9 , and four saponins, 10 , 11 , 12 , and 14 , exhibited high permeability across the monolayers. The results indicated that a higher degree of glycosylation-facilitated permeability, suggestive of active transport. This study demonstrates the utility of the Caco-2 permeability assay as a method of identifying possible bioavailable compounds from medicinal herbal extracts.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Reports suggest that approximately 165 saponins are present in G. pentaphyllum . 18 , 21 The major saponins present in G. pentaphyllum belong to the dammarane class of saponins (known as gypenosides, if isolated from G. pentaphyllum ). Dammarane saponins are characterized by a dammarane aglycone with an acyclic side chain ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Reports suggest that approximately 165 saponins are present in G. pentaphyllum. 18,21 The major saponins present in G. pentaphyllum belong to the dammarane class of saponins (known as gypenosides, if isolated from G. A variety of flavonoids have also been isolated from G. pentaphyllum with the free aglycone forms (quercetin, kaempferol, and isorhamnetin) being reported; 21,22 however, the most prominent flavonoids present in G. pentaphyllum are found as quercetin and kaempferol glycosides (Figure 2). 22 A large number of studies have already established the diverse pharmacological benefits of organic or aqueous crude extracts of G. pentaphyllum or of a saponin-rich extract preparation.…”

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Caco-2 Cell Permeability of Flavonoids and Saponins from Gynostemma pentaphyllum: the Immortal Herb

Ahmed

Leach²,

Wohlmuth

et al. 2020

ACS Omega

View full text Add to dashboard Cite

show abstract

Isolation and purification of high polar glycosides from aerial parts of Gynostemma pentaphyllum (Thunb.) Makino by linear gradient counter‐current chromatography coupled with inner‐recycling mode

Sun,

Xu,

Yan

et al. 2023

J of Separation Science

View full text Add to dashboard Cite

Gynostemma pentaphyllum (Thunb.) Makino represents the popular health food and supplemental product with broad pharmacological activities. The highly polar glycosides, including flavonoids and saponins, are major effective active components that contain diverse sugar positions and quantities, which result in diverse chemical polarities, making it challenging to separate and isolate these components. The present work described the rapid and efficient linear gradient counter‐current chromatography to preparatively separate glycosides from aboveground parts of G. pentaphyllum. Besides, the ethyl acetate and n‐butanol binary mobile phases were achieved through adjusting associated proportions. Six glycosides, including quercetin‐3‐O‐neohesperidoside (1), kaempferol‐3‐O‐robinobioside (2), kaempferol‐3‐O‐neohesperidoside (3), gypenoside LVI (4), ginsenoside Rb3 (5), and gypenoside XLVI (6), were isolated at the purities greater than 98%. Moreover, electrospray ionization mass spectrometry and nuclear magnetic resonance tandem mass spectrometry were conducted for structural identification. According to our findings, the established linear gradient counter‐current chromatography was an efficient approach to separate the highly polar glycosides from aboveground parts of G. pentaphyllum. Our proposed strategy can be used to separate active compounds from other complex natural products.

show abstract

Countercurrent Chromatography—When Liquid-Liquid Extraction Meets Chromatography

Vetter

Müller

Englert

et al. 2020

Liquid-Phase Extraction

View full text Add to dashboard Cite

Effective and Preparative Separation of Bioactive Flavonoids FromGynostemma PentaphyllumTea Using Elution-Extrusion Counter-Current Chromatography

Cited by 6 publications

References 21 publications

Caco-2 Cell Permeability of Flavonoids and Saponins from Gynostemma pentaphyllum: the Immortal Herb

Caco-2 Cell Permeability of Flavonoids and Saponins from Gynostemma pentaphyllum: the Immortal Herb

Isolation and purification of high polar glycosides from aerial parts of Gynostemma pentaphyllum (Thunb.) Makino by linear gradient counter‐current chromatography coupled with inner‐recycling mode

Countercurrent Chromatography—When Liquid-Liquid Extraction Meets Chromatography

Contact Info

Product

Resources

About