EFFECT OF Α-Tocopherol SUPPLEMENTATION IN THE EXTENDER ON THE SPERM QUALITY OF MADURAN BULL BEFORE AND AFTER QUICK FREEZING.

Ratnani, Hermin; Ihsan, MN; Ciptadi, Gatot; Suyadi,

doi:10.21474/ijar01/4849

Cited by 7 publications

(7 citation statements)

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“…Eriani et al (2018) reported the use of β-carotene supplementation in freezing semen of buffalo. In addition, tocopherol supplementation had a beneficial effect on sperm motility on Maduran bulls (Ratnani et al, 2017). Other studies report that the natural antioxidants are being used in semen freezing, such as Nigella Sativa oil (Maidin et al, 2018), Moringaceae extract (Syarifuddin et al, 2017), and curcumin in cryopreservation of bovine semen (Bucak et al, 2012).…”

Section: Introductionmentioning

confidence: 96%

Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

Nurcholis¹,

Furqon

Arifiantini

et al. 2021

Trop. Anim. Sci. J.

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Antioxidants such as tocopherol, ß-carotene, and polyunsaturated fatty acids (PUFA) from red fruit oil of Papua may be used to protect frozen semen. The study is aimed to test the effect of red fruit oil supplementation on motility, viability, and recovery rate of frozen sperm of Ongole-grade bulls. Semen was collected twice a month from eight 4-5-year-old male Ongole grade using an artificial vagina, followed by macro- and microscopical evaluations. Collected semen was divided into four tubes and diluted with tris egg yolk diluents (TEY) as a control, TEY supplemented with 0.5% red fruit oil (RFO) (TEY RFO0.5), TEY supplemented with 1% RFO (TEY RFO1.0), and TEY supplemented with 1.5% RFO (TEY RFO1.5). The diluted semen was then packed into the straw and equilibrated for 2, 4, and 6 h prior to frozen on liquid nitrogen vapor for 10 minutes. The observed variables in this study were sperm motility, sperm viability, and morphology after equilibration, after thawing, and recovery rate. The experimental design is a completely randomized factorial design. The data were analyzed using ANOVA and were further tested using Duncan multiple range test. The results showed that the sperm motility of fresh semen was 81.10±1.42%. The percentage of sperm motility in TEY RFO1.5 treatment at 6 h equilibration was 60.00±1.06%, significantly higher compared to TEY RFO1.0 and TEY RFO0.5. The percentage of post-thawing sperm motility in TEY RFO1.5 treatment was 62.40±1.09%. The best post-thawing sperm viability in TEY RFO1.5 was 80.70±1.20%, significantly increase from the treatment of TEY RFO1.0 and TEY RFO0.5. The recovery rate (RR) for TEY RFO1.5 treatment had the best percentage at 76.94%. In conclusion, RFO supplementation in semen diluents for 2 h of equilibration resulted in the best motility and viability at 0 h of post thawing observation.

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Section: Introductionmentioning

confidence: 96%

Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

Nurcholis¹,

Furqon

Arifiantini

et al. 2021

Trop. Anim. Sci. J.

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“…Cryoprotectants such as egg yolk and soybean or milk have different antioxidant capacity (Alvarez-Rodríguez et al, 2013) either way; the results of previous studies corresponded with the result of the present study. The supplementation α-tocopherol in egg yolk tris aminomethane diluent resulted in beneficial effect on spermatozoa motility and plasma membrane integrity in Madura bull (Ratnani et al, 2017). A lso α-tocopherol can be an efficient antioxidant additive in Bioxcell extender for cryopreservation of bull semen (Motemani et al, 2017).…”

Section: Discussionmentioning

confidence: 97%

Effect of α-tocopherol supplementation in diluents on the motility, viability and plasma membrane integrity of Simmental bull spermatozoa after cooling

Azura¹,

Ratnani²,

Susilowati³

et al. 2020

Ovz

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Semen storage in cold temperatures might cause an increase in reactive oxygen species (ROS) production. This condition resulted in spermatozoa damage and quality decrease. This study was conducted to investigate the effects of α-tocopherol supplementation in diluents on the motility, viability, and plasma membrane integrity of Simmental bull spermatozoa after cooling. Semen samples were diluted in skim milk egg yolk supplemented with 0, 0.5, 1.0, and 1.5 mM α-tocopherol respectively for control, Tl, T2, and T3. Spermatozoa were evaluated for their motility, viability, and membrane integrity in cooling temperature (5°C). The daily evaluation showed that 1.5 mM α-tocopherol was the best in maintaining motility, viability, and plasma membrane integrity, while 1.0 mM α-tocopherol was only good for maintaining viability. Therefore, it can be concluded that α-tocopherol at the concentration of 1.5 mM was an efficient antioxidant supplement for Simmental cattle semen in skim milk egg yolk diluent.

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“…Sperm membranes are mostly composed of Polyunsaturated Fatty Acids (PUFA) that makes the sperm is very susceptible to lipid peroxidation. Vitamin E supplementation helps in maintaining the sperm motility of cooled semen of bulls (Ratnani et al, 2017). This helps in finding free radicals created from lipid peroxidation by creating two new stable radicals from one vitamin E molecule (Hajibabaei, 2016).…”

Section: Discussionmentioning

confidence: 99%

The Protective Impacts of α-tocopherol Supplementation on the Semen Quality of Sapera Goat Preserved at 4oC

Prastiya

Rimayanti

Munir

et al. 2021

Trop. Anim. Sci. J.

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Supplementation of vitamin E (α-tocopherol) as an antioxidant into an extender is useful to maintain sperm quality during the process of cryopreservation by protecting the sperms from reactive oxygen species (ROS). This study aims to evaluate the protective impacts of vitamin E supplementation on the motility, viability, and reducing the abnormality of sperms in Sapera goats. The sample of semen used in the present study was obtained from fresh semen and good-quality ejaculates of the Sapera goats collected by an artificial vagina. The groups involve one controlled treatment group, which contains semen and tris egg yolk (TEY) extender without vitamin E (P0), and three treatment groups that contain semen, TEY extender, and different doses of vitamin E, i.e., 1 mg/mL (P1), 2 mg/mL (P2), and 3 mg/mL (P3), respectively. Data obtained from the experiments were analyzed using Repeated Measures Multivariate Analysis (MANOVA). The results showed that TEY extenders added with vitamin E at a dose of 1 mg/mL of vitamin E (P1) produced the highest motility and viability with the lowest abnormalities of sperm after 24 th hour of cryopreservation. The lowest sperm motility and viability among the treatment groups was observed in the TEY extender added with vitamin E at a dose of 3 mg/mL (P3) at 96 th hour after cryopreservation, which was almost the same as the control group without vitamin E (P0) that had the lowest motility and viability. In conclusion, the current study found that supplementation of vitamin E as an antioxidant at a dose of 1 mg/mL in TEY extender can preserve semen of the Sapera goats in refrigerator up to 96 h at 4°C and provides higher sperm motility and viability percentage, with a lower percentage of sperm abnormality.

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EFFECT OF Α-Tocopherol SUPPLEMENTATION IN THE EXTENDER ON THE SPERM QUALITY OF MADURAN BULL BEFORE AND AFTER QUICK FREEZING.

Cited by 7 publications

References 0 publications

Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

Effect of α-tocopherol supplementation in diluents on the motility, viability and plasma membrane integrity of Simmental bull spermatozoa after cooling

The Protective Impacts of α-tocopherol Supplementation on the Semen Quality of Sapera Goat Preserved at 4oC

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