Effect of ZnCl2 on plaque growth and biofilm vitality

Gu, Hao; Fan, Danni; Gao, Jinlong; Zou, Wei; Peng, Zhixiang; Zhao, Ziming; Ling, Junqi; LeGeros, Racquel Z.

doi:10.1016/j.archoralbio.2011.10.001

Cited by 52 publications

(55 citation statements)

References 23 publications

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“…In the present study, RSM was used to optimize the amount of four diff erent antimicrobial agents which could inhibit the growth of biofi lm based on preliminary steps (single factor test and orthogonal test) and optimum antimicrobial agents (2.16 g/L of ZnSO 4 , 14 g/L of lysozyme, 4.5 g/L of citric acid and 5 g/L of chitosan), from which the desired inhibition ratio of biofi lm was obtained. This is in accordance with Gu et al (2012), who reported that mouthrinse with zinc at a concentration of 2.5 mmol/L or 5.0 mmol/L has signifi cant antibacterial and plaque control eff ects on the outer and middle layers of the biofi lm in vivo. From the results of the orthogonal test and RSM, it is worthwhile to mention that the infl uence of ZnSO 4 when compared with the other factors was not so signifi cant on the inhibition ratio of biofi lm with increasing concentrations.…”

Section: Discussionsupporting

confidence: 92%

“…As shown in Fig.1b, when the lysozyme independently infl uenced the biofi lm, the inhibition ratio of biofi lm was very low. This phenomenon may be because a zinc ion can activate a particular part of lysozyme which can more eff ectively increase the inhibition ratio of lysozyme (He et al, 2002;Burguera-Pascu et al, 2007), but no signifi cant inhibition of vitality was observed at high concentrations, which is in accordance with the report by Gu et al (2012). Because of this reason, ZnSO 4 continued to be used in the following experiment.…”

Section: Discussionsupporting

confidence: 81%

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Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash

Ren

Wang

et al. 2015

Chin. J. Ocean. Limnol.

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We adopted the response surface methodology using single factor and orthogonal experiments to optimize four types of antimicrobial agents that could inhibit biofi lm formation by Streptococcus mutans , which is commonly found in the human oral cavity and causes tooth decay. The objective was to improve the function of marine Arthrobacter oxydans KQ11 dextranase mouthwash (designed and developed by our laboratory). The experiment was conducted in a three-level, four-variable central composite design to determine the best combination of ZnSO 4 , lysozyme, citric acid and chitosan. The optimized antibacterial agents were 2.16 g/L ZnSO 4 , 14 g/L lysozyme, 4.5 g/L citric acid and 5 g/L chitosan. The biofi lm formation inhibition reached 84.49%. In addition, microscopic observation of the biofi lm was performed using scanning electron microscopy and confocal laser scanning microscopy. The optimized formula was tested in marine dextranase Arthrobacter oxydans KQ11 mouthwash and enhanced the inhibition of S . mutans . This work may be promoted for the design and development of future marine dextranase oral care products.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 81%

Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash

Ren

Wang

et al. 2015

Chin. J. Ocean. Limnol.

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“…In Paper I, II and IV the active ingredients in the mouth rinse used were zinc acetate and chlorhexidine diacetate. Zinc and chlorhexidine have been reported to have antibacterial effects (Roldan et al 2004, Cousido et al 2010, Gu et al 2012.…”

Section: Discussionmentioning

confidence: 99%

“…Mouth rinses containing metal salts, essential oils, chlorhexidine, chlorine dioxide and cetylpyridinium chloride in different combinations have been shown to reduce VSCs in exhaled air (Pitts et al 1983, Kozlovsky et al 1996, Frascella et al 2000, Silwood et al 2001, Young et al 2001, Borden et al 2002, Peruzzo et al 2007). Metal salts, essential oils, chlorhexidine, chlorine dioxide and cetylpyridinium chloride are also known to have an antibacterial effects (Young et al 2001& 2003, Roldan et al 2003& 2004, Sreenivasan et al 2005, Gu et al 2012. Data have shown that zinc (Dadamio et al 2013b), clorindioxid (Silwod et al 2001) and CHX (Quirynen et al 2005) reduces VSC levels in exhaled air.…”

Section: Mouth Rinsingmentioning

confidence: 99%

The effect of periodontal therapy on intra‐oral halitosis: a case series

Ademovski

Mårtensson

Persson

et al. 2016

J Clinic Periodontology

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Aim The aim of this study was to evaluate the effects of non‐surgical periodontal therapy on intra‐oral halitosis 3 months after therapy. Material and methods Sixty‐eight adults with intra‐oral halitosis were included in a case series. Intra‐oral halitosis was evaluated at baseline, and at 3 months after treatment using the organoleptic scores (OLS), Halimeter®, and a gas chromatograph. Results Significant reductions for OLS (p < 0.01), total sum of volatile sulphur compounds (T‐VSC) (p < 0.01) and methyl mercaptan (MM) (p < 0.05) values were found after treatment. Hydrogen sulphide (H2S) levels were not significantly reduced. The numbers of probing pockets 4 mm, 5 mm and 6 mm were significantly reduced as a result of therapy (p < 0.001). Bleeding on probing (BOP) and plaque indices were also significantly reduced (p < 0.001). For the 34 individuals with successful periodontal treatment (BOP<20% and a ≥50% reduction of total pocket depth) reductions in OLS (p < 0.01) and T‐VSC scores (p < 0.01) were found. Eleven individuals were considered effectively treated for intra‐oral halitosis presenting with a T‐VSC value <160 ppb, a H2S value <112 ppb and a MM value <26 ppb. Conclusion Non‐surgical periodontal therapy resulted in reduction of OLS, MM and T‐VSC values 3 months after therapy. Few individuals were considered as effectively treated for intra‐oral halitosis.

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“…Several nanoparticles (eg, zinc oxide, 60,61 silver, [62][63][64] and polyethylenimine 65 ) have been incorporated into dental composites 66 or dental adhesives 67 to inhibit the bacterial growth through several mechanisms. These mechanisms include disruption of the bacterial cell membrane, 68 inhibition of the active transport as well as the metabolism of sugars, generation of reactive oxygen species, 69 displacement of magnesium ions required for the enzymatic activity of oral biofilms, 70 disturbance of the electron transportation across the bacterial membrane, 63 and prevention of DNA replication.…”

Section: Antibacterial Nanotherapymentioning

confidence: 99%

Nanotechnology in dentistry: prevention, diagnosis, and therapy

Neel¹,

Bozec

Pérez³

et al. 2015

IJN

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Nanotechnology has rapidly expanded into all areas of science; it offers significant alternative ways to solve scientific and medical questions and problems. In dentistry, nanotechnology has been exploited in the development of restorative materials with some significant success. This review discusses nanointerfaces that could compromise the longevity of dental restorations, and how nanotechnolgy has been employed to modify them for providing long-term successful restorations. It also focuses on some challenging areas in dentistry, eg, oral biofilm and cancers, and how nanotechnology overcomes these challenges. The recent advances in nanodentistry and innovations in oral health-related diagnostic, preventive, and therapeutic methods required to maintain and obtain perfect oral health, have been discussed. The recent advances in nanotechnology could hold promise in bringing a paradigm shift in dental field. Although there are numerous complex therapies being developed to treat many diseases, their clinical use requires careful consideration of the expense of synthesis and implementation.

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Effect of ZnCl2 on plaque growth and biofilm vitality

Cited by 52 publications

References 23 publications

Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash

Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash

The effect of periodontal therapy on intra‐oral halitosis: a case series

Nanotechnology in dentistry: prevention, diagnosis, and therapy

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