Effect of Zinc Sulfate and Zinc Glycine Chelate on Concentrations of Acute Phase Proteins in Chicken Serum and Liver Tissue

Abstract

The aim of the study was to determine how inorganic and organic forms of zinc affect the concentrations of C-reactive protein (CRP), serum amyloid A (SAA), alpha-1-acid glycoprotein (α-1-AGP), haptoglobin (Hp), and transferrin (TRF) in the blood and liver tissue of 450 1-day-old Ross 308 chicken. Four experimental groups received one the following: inorganic zinc (ZnSO 4 ), a zinc phytase enzyme supplement (ZnSO 4 -F), organic zinc in combination with glycine (Zn-Gly), or organic zinc supplemented with phytase (Zn-Gly-F). The chicken serum and liver homogenates were assayed using an ELISA kit. The results of the study showed statistically significantly higher serum and liver concentration of SAA in the group of birds that received zinc sulfate in comparison to the group of birds receiving zinc in organic form. A statistically significantly higher serum concentration of CRP and α-1-AGP was also noted in the group receiving zinc sulfate as compared to the Zn-Gly group. Comparison of the serum concentration of TRF between the supplemented groups showed a statistically significant increase in this parameter in the ZnGly-F group as compared to the ZSO 4 -F group. The increase in the serum concentration of Hp in all groups in comparison to the control may indicate stimulation of local immune mechanisms. The results of this study showed an increase in the concentrations of APPs such as AGP and TRF following the administration of zinc glycine chelates, which may demonstrate their effect on metabolic processes in the liver and on immunocompetent cells that regulate the intensity of the immune response.

Effect of Zinc Source and Exogenous Enzymes Supplementation on Zinc Status in Dogs Fed High Phytate Diets

“…Even though Zn supplementation is more efficient decreasing the CRP of ill patients, basal levels of CRP can also benefit from Zn supplementation [51]. A study of Jarosz et al reported that chelate of Zn-glycine reduced the basal level of CRP in comparison to Zn sulfate in chickens from 1 to 42 days of age [52]. However, in the results reported here, the basal levels of CRP in dogs were not affected by Zn supplementation.…”

Effects of dietary zinc source on the metabolic and immunological response to lipopolysaccharide in lactating Holstein dairy cows

“…In the present study, feeding complexed Zn increased circulating SAA post-LPS infusion. Similarly, feeding complexed Zn increased APP production in heat-stressed calves and pigs (Pearce et al, 2015;Abuajamieh et al, 2016), chickens (Jarosz et al, 2019), and transitioning dairy cows (Batistel et al, 2016). Interestingly, Liu et al (2014) demonstrated that Zn deficiency enhances APP production via activating the Table 5.…”

Effects of Bacillus subtilis, butyrate, mannan-oligosaccharide, and naked oat (ß-glucans) on growth performance, serum parameters, and gut health of broiler chickens

“…Because CRP is an acute-phase protein in inflammatory responses, and it is stimulated by the presence of both interleucine-1 ( IL-1 ) and interleucine-6 ( IL-6 ), such increase might indicate the development of a local inflammatory process ( Eckersall and Bell, 2010 ). An increase in the concentration of CRP is associated with cell damage caused by pathogens, that within 36 to 48 h postexposure to such stimulus ( Jarosz et al, 2019 ). The reduced growth performance in Bacillus subtilis treatment coincides with the reduction in AMEn and digestible Lys and other amino acids supplied in ratio with Lys in grower and finisher diet.…”