Effect of zinc oxide (ZnO) nanoparticles on physiology and steviol glycosides production in micropropagated shoots of Stevia rebaudiana Bertoni

Javed, Rabia; Usman, Muhammad; Yücesan, Buhara; Zia, Muhammad; Gürel, Ekrem

doi:10.1016/j.plaphy.2016.05.032

Cited by 141 publications

(73 citation statements)

References 26 publications

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“…Previously, the influence of these ENPs on the in vitro grown shoots of Candyleaf has been documented. Those findings coincide with the current results where maximum threshold was achieved at 1 mg/L and 10 mg/L dosages of ZnO and CuO ENPs, respectively [34][35][36]. It is comprehended that an almost equal ENPs (ZnO and CuO) stress has been tolerated by the Candyleaf shoot and root organs.…”

Section: Discussionsupporting

confidence: 91%

“…Molecules 2020, 25, x FOR PEER REVIEW 3 of 14 only in the shoots of Candyleaf grown by tissue culture [34,35]. However, the impact that both these ENPs create on in vitro rooting of this highly valuable medicinal plant has not been exploited so far.…”

Section: Characterization Of Enpsmentioning

confidence: 99%

“…Candyleaf seeds were purchased from Shenyang Agricultural University (China). According to the [34] protocol, seeds were disinfected with 0.1% (w/v) of mercuric (II) chloride (HgCl 2 ) and then cultured on plain MS medium. From 4 weeks old seedlings, axillary shoot nodes were removed and incubated in MS medium (without any plant growth regulators (PGRs)) for shoots formation.…”

Section: Growth Conditions Of Organogenesismentioning

confidence: 99%

“…According to the protocol of [34], leaves from regenerants were taken to extract SGs. The process involved washing of leaves, taken from different treatments, with sterile distilled water.…”

Section: Steviol Glycosides (Sgs) Analysismentioning

confidence: 99%

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Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)

et al. 2020

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Sustainable production of secondary metabolites in medicinal plants by artificial culturing on the industrial scale has gained worldwide importance. Engineered nanoparticles (ENPs) play a pivotal role in the elicitation of compounds of medicinal value. This investigation explores the influence of ZnO and CuO ENPs on in vitro roots formation, non-enzymatic antioxidant activities, and production of steviol glycosides (SGs) in regenerants of Candyleaf, Stevia rebaudiana. ENPs were applied in 0, 2, 20, 200, and 2000 mg/L of concentration in the MS medium containing plant shoots. The percentage of rooting induced was 91% and 94% by applying ZnO ENPs (2 mg/L) and CuO ENPs (20 mg/L), respectively. Moreover, at 2 mg/L of ZnO and 20 mg/L of CuO ENPs, the high performance liquid chromatography studies determined the significantly greatest content of SGs; rebaudioside A (4.42 and 4.44) and stevioside (1.28 and 1.96). Phytochemical studies including total flavonoid content, total phenolic content, and 2,2-diphenyl-1-picryl hydrazyl-free radical scavenging activity were calculated highest by the regenerants grown in 2 mg/L of ZnO and 20 mg/L of CuO ENPs dosage. Both ZnO and CuO ENPs at 200 mg/L and 2000 mg/L of concentration induced adverse effects on plant biomass, antioxidant activities, and SGs content up to 1.22 and 1.77 for rebaudioside A and 0.21 and 0.25 for stevioside. Hence, the biochemical and morphophysiological responses of Candyleaf were elicited as a defense against ZnO and CuO ENPs applied under threshold limit. This artificial biotechnological technique holds great promise for continued production of natural antioxidants on commercial scale and our study has further strengthened this impact.

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Section: Discussionsupporting

confidence: 91%

Section: Characterization Of Enpsmentioning

confidence: 99%

Section: Growth Conditions Of Organogenesismentioning

confidence: 99%

Section: Steviol Glycosides (Sgs) Analysismentioning

confidence: 99%

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Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)

et al. 2020

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“…We obtained the highest lignans content/production (SDG 25.01 mg/g and 284.12 mg/l; LDG 7.65 mg/g and 86.97 mg/l) after 25 days of initial culturing when cell suspension cultures were repeatedly treated with ZnO-NPs on day 0 and 15; whereas highest neolignans content/production (DCG 36.44 mg/g and 493.28 mg/l; GCGG 22.73 mg/g and 307.69 mg/l) occurred after 30 days of initial culturing when cell suspension cultures were repeatedly treated with ZnO-NPs on day 0 and 25 (Tables 1 and 2). The mechanism underlying enhanced production of secondary metabolites in response to ZnO-NPs is still unclear, but a number of reports are available on their role as a potential abiotic elicitor during in vitro cultures such as Sharafi et al [33], Karimi et al [21] and Javed et al [34]. Bhardwaj et al [35] made an attempt to decipher the transcriptional regulation of ZnO-NPs induced enhanced accumulation of bacoside A content in cell suspension cultures of Bacopa monnieri.…”

Section: Resultsmentioning

confidence: 99%

Biogenic zinc oxide nanoparticles-enhanced biosynthesis of lignans and neolignans in cell suspension cultures of Linum usitatissimum L

Abbasi

Zahir

Ahmad

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Zinc oxide nanoparticles (NPs) have emerged as a novel elicitor for enhanced biosynthesis of secondary metabolites in in vitro plant cell cultures. The current study was aimed to explore elicitation abilities of ZnO-NPs for enhanced accumulation of lignans and neolignans in cell cultures of Linum usitatissimum. We optimized concentration of zinc oxide NPs before carrying out a full-fledged experiment. Subsequently, an optimum dose of 100 mg/l was introduced into the culture medium on day 0, days 0 and 15, and finally days 0 and 25. We observed that repeated elicitation stimulated various parameters and physiological responses in Linum usitatissimum cell cultures than one-time elicitation. Repeated elicitation of cell cultures on day 0 and 15 resulted in highest fresh weight (412.16 g/l) and lignans production (secoisolariciresinol diglucoside 284.12 mg/l: lariciresinol diglucoside 86.97 mg/l). Contrarily, repeated elicitation on day 0 and 25 resulted in highest DW (13.53 g/l), total phenolic production (537.44 mg/l), total flavonoid production (123.83 mg/l) and neolignans production (dehydrodiconiferyl alcohol glucoside 493.28 mg/l: guaiacylglycerol-b-coniferyl alcohol ether glucoside 307.69 mg/l). Enhancement in plant growth and secondary metabolites accumulation was several fold higher than controls. Furthermore, a linear relationship existed between total phenolic and flavonoid contents which in turn was correlated with higher antioxidant activities. ARTICLE HISTORY

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Nanoparticles as Elicitors of Biologically Active Ingredients in Plants

Anjum

Komal

Abbasi

et al. 2021

Nanotechnology in Plant Growth Promotion and Protection

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Effect of zinc oxide (ZnO) nanoparticles on physiology and steviol glycosides production in micropropagated shoots of Stevia rebaudiana Bertoni

Cited by 141 publications

References 26 publications

Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)

Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)

Biogenic zinc oxide nanoparticles-enhanced biosynthesis of lignans and neolignans in cell suspension cultures of Linum usitatissimum L

Nanoparticles as Elicitors of Biologically Active Ingredients in Plants

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