Effect of zinc on in vitro development of porcine embryos

Jeon, Yubyeol; Yoon, Jong Hwan; Cai, Lei; Hwang, Seon‐Ung; Kim, Eunhye; Lee, Eunsong; Jeung, Eui Bae; Hyun, Sang‐Hwan

doi:10.1016/j.theriogenology.2015.04.006

Cited by 12 publications

(9 citation statements)

References 25 publications

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“…It is reported that cleavage rate of porcine IVF embryos is 51.02%–71.43% and the blastocyst development rate is 14.5%–19.93% (Do et al, ; Jeona et al, ; Kim et al, ; Liu et al, ; Miriam et al, ; Sakurai et al, ; Yuan et al, ). Therefore, it is important to explore the influence on various intrinsic factors and mechanisms of porcine early embryo development, in order to improve the quality of porcine embryo development in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…It is reported that cleavage rate of porcine IVF embryos is 51.02%-71.43% and the blastocyst development rate is 14.5%-19.93% (Do et al, 2015;Jeona et al, 2015;Kim et al, 2017;Liu et al, 2018;Miriam et al, 2014;Sakurai et al, 2015;Yuan et al, 2014 It is reported that the development of pre-implantation embryos in mouse is related to the expression and synthesis of miRNAs (Ohnishi et al, 2010). A total of 214 different miR-NAs have been found to be involved in the regulation of mouse pre-implantation embryo development (Mineno et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…The development rate of bovine blastocysts cultured in vitro has been reported to be 45.29% (Ayman et al, 2017), while the rate of 14. 5%-19.93% was reported in pigs (Do et al, 2015;Jeona et al, 2015;Kim et al, 2017;Liu, Liu, Larsen, Hou, & Callesen, 2018;Miriam, Sergi, Roser, & Marc, 2014;Sakurai, Suzuki, & Yoshioka, 2015;Yuan, Paczkowski, Wheeler, & Krisher, 2014). The development of human-assisted reproduction and progress in animal production are greatly hindered by these limitations in pre-implantation embryo development.…”

Section: Backg Rou N Dmentioning

confidence: 99%

“…Studies have shown that 50% of human embryos cultured in vitro stop developing within the first week (FitzGerald & DiMattina, ), and the development rate of high‐quality blastocysts is about 30% (Hu et al, ). The development rate of bovine blastocysts cultured in vitro has been reported to be 45.29% (Ayman et al, ), while the rate of 14.5%–19.93% was reported in pigs (Do et al, ; Jeona et al, ; Kim et al, ; Liu, Liu, Larsen, Hou, & Callesen, ; Miriam, Sergi, Roser, & Marc, ; Sakurai, Suzuki, & Yoshioka, ; Yuan, Paczkowski, Wheeler, & Krisher, ). The development of human‐assisted reproduction and progress in animal production are greatly hindered by these limitations in pre‐implantation embryo development.…”

Section: Introductionmentioning

confidence: 99%

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Function of berberine on porcine in vitro fertilization embryo development and differential expression analysis of microRNAs

Wang

Zhang

Liu

et al. 2019

Reprod Domestic Animals

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Contents The effect of berberine (Ber) on in vitro fertilization (IVF) embryo development in pigs and the associated differential expression of microRNAs (miRNAs) in the embryo were investigated. NCSU‐23 embryonic culture medium was used for a control group, while NCSU‐23 embryonic culture medium added with Ber was used for a Ber group. The embryo development rates in these groups were determined, and the zygotes, 4‐ and 8‐cell embryos, and blastocysts were collected for cDNA microarray analysis. The development rates of 2‐, 4‐, 8‐cell embryos and blastocysts were significantly higher in the Ber group than those in the control group (p < 0.01). The differentially expressed miRNAs in the 8‐cell versus the 4‐cell stage in control group as well as in the 8‐cell Ber group versus the 8‐cell control group overlapped, and it was found that nine miRNAs were commonly upregulated and two of them were downregulated, while there was no overlap among the other groups. The target genes of Ber‐regulated miRNAs at the 8‐cell stage were mainly associated with the molecular pathway of nucleic acid and protein synthesis. These findings suggest that Ber may regulate the expression of miRNAs at the 8‐cell stage, which is beneficial to provide material reserves for the maternal to zygote transition of porcine embryos, thereby increasing the porcine IVF embryo development rate.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Backg Rou N Dmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Function of berberine on porcine in vitro fertilization embryo development and differential expression analysis of microRNAs

Wang

Zhang

Liu

et al. 2019

Reprod Domestic Animals

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“…For example, Klf4, a zinc finger transcription factor, is an essential member of the core pluripotency transcriptional network in ES cells [ 27 – 29 ]. A recent study demonstrated that zinc was required for embryonic development, and supplementation with adequate zinc improved the viability of porcine embryos in vitro [ 30 ]. Commercially available culture media for mouse ES cells do not contain any zinc ion.…”

Section: Introductionmentioning

confidence: 99%

Zinc Chloride Transiently Maintains Mouse Embryonic Stem Cell Pluripotency by Activating Stat3 Signaling

Yang

Wang

et al. 2016

PLoS ONE

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An improved understanding of the pluripotency maintenance of embryonic stem (ES) cells is important for investigations of early embryo development and for cell replacement therapy, but the mechanism behind pluripotency is still incompletely understood. Recent findings show that zinc, an essential trace element in humans, is critically involved in regulating various signaling pathways and genes expression. However, its role in ES cell fate determination remains to be further explored. Here we showed that 2μM zinc chloride (ZnCl2) transiently maintained mouse ES cell pluripotency in vitro. The cultured mouse ES cells remained undifferentiated under 2μM ZnCl2 treatment in leukemia inhibitory factor (LIF) withdrawal, retinoic acid (RA) or embryoid bodies (EBs) differentiation assays. In addition, ZnCl2 increased pluripotency genes expression and inhibited differentiation genes expression. Further mechanistic studies revealed that ZnCl2 transiently activated signal transducers and activators of transcription 3 (Stat3) signaling through promoting Stat3 phosphorylation. Inhibition of Stat3 signaling abrogated the effects of ZnCl2 on mouse ES cell pluripotency. Taken together, this study demonstrated a critical role of zinc in the pluripotency maintenance of mouse ES cells, as well as an important regulator of Stat3 signaling.

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Zinc supplementation of vitrification medium improves in vitro maturation and fertilization of oocytes derived from vitrified-warmed mouse ovaries

et al. 2017

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Effect of zinc on in vitro development of porcine embryos

Cited by 12 publications

References 25 publications

Function of berberine on porcine in vitro fertilization embryo development and differential expression analysis of microRNAs

Function of berberine on porcine in vitro fertilization embryo development and differential expression analysis of microRNAs

Zinc Chloride Transiently Maintains Mouse Embryonic Stem Cell Pluripotency by Activating Stat3 Signaling

Zinc supplementation of vitrification medium improves in vitro maturation and fertilization of oocytes derived from vitrified-warmed mouse ovaries

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