Effect of Zinc Metal at High Concnetration on Secondary Metabolic Pathways in Penicillium Chrysogenum Strain

Bourzama, Ghania; Ennaghra, Nadjet; Soumati, Boudjma; Benoune, Saliha; Atriche, Nesrine

doi:10.15414/jmbfs.2019.9.2.307-313

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…Our results agreed with those of several studies that associated the presence of these species in contaminated soils with the bioabsorption of metals. It has been reported that A. fischeri is related to bioabsorption of Cd (Babich & Stotzky, 1977), A. fumigatus to bioabsorption of several metals such as Cu, Cr and Pb (Shazia, Uzma, & Talat, 2013), and A. clavatus to bioabsorption of Pb (Bourzama, Ennaghra, Soumati, Benoune, & Atriche, 2019). The important role of these species in metal absorption may explain their high abundance in the most contaminated samples (B and C).…”

Section: Structure Of the Microbial Communitymentioning

confidence: 98%

Taxonomic and functional analysis of soil microbial communities in a mining site across a metal(loid) contamination gradient

Navas

Pérez-Esteban

Torres

et al. 2020

European J Soil Science

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Soil microorganisms surviving in mining sites have developed metalresistance mechanisms to biotransform metals. Their use as biofertilizers can improve phytoremediation efficiency in contaminated soils by reducing metal toxicity while promoting plant growth. We analysed through wholemetagenome shotgun sequencing the composition, diversity and function of microbial communities present in a contaminated mine soil along a gradient of metals (As, Cu, Fe, Mn, Pb, Zn) to identify tolerant species carrying metal-resistance functional genes that can be used in association with plants for phytoremediation. Soil samples were collected from an abandoned copper mine, across three areas with different levels of metal contamination (unaffected, moderately contaminated and highly contaminated). The relative abundance of Proteobacteria, especially genus Bradyrhizobium, increased in the highly contaminated area, whereas Actinobacteria dominated in the unaffected area. Archaea (Euryarchaeota) predominated in the moderately contaminated area, Haloarcula, Halobacterium and Halorubrum being the most abundant genera. The fungi Basidiomycota did not exhibit differences among the areas, whereas Ascomycota, especially the genus Aspergillus, increased in areas with low metal concentrations. Metal-resistance genes associated with Fe (acn, furA, dpsA), Cu (cop-unnamed, copF, actP, copA, mmco, cutO) and As (arsT, arsC, aioA/aoxB) metabolism were the most abundant and were affected by the gradient of soil contamination. Those associated with Cu predominated in the most contaminated area, whereas As and Fe genes were more abundant in the least contaminated. Among the carriers of these metal-resistance genes, Bradyrhizobium diazoefficiens, Pseudomonas aeruginosa, Halorubrum trapanicum, Aspergillus fumigatus and A. fischeri were dominant in the most contaminated area. These species could give rise to promising biofertilizers to be used in association with suitable plants for the phytoremediation of contaminated mining sites.

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Section: Structure Of the Microbial Communitymentioning

confidence: 98%

Taxonomic and functional analysis of soil microbial communities in a mining site across a metal(loid) contamination gradient

Navas

Pérez-Esteban

Torres

et al. 2020

European J Soil Science

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“…This part was carried out since the growth of fungal strains on unconventional carbon sources could modify the mycelium morphology, these modifications are observable with a light microscope (Bourzama et al, 2019). The mycelium parts were spread out on a glass slide with sterile normal saline, and observed under a light microscope in order to observe the modifications shown on hyphal tips in comparaison with the observation of the same fungal strain on conventional carbon source (grown on PDA).…”

Section: Microscopic Observationmentioning

confidence: 99%

In Vitro Removal of Electronic and Electrical Wastes by Fungi Isolated from Soil at Annaba Area in Northeast of Algeria

Bourzama

Iratni

Ennaghra

et al. 2021

Environ. Nat. Resour. J.

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Electronic and electrical wastes (EEW) have increased exponentially in recent years due to technological progress. The uncontrolled incineration of these wastes causes pollution of air, soil, and water that has dangerous effects on health of human beings and other living organisms. This work isolated fungi that are capable of degrading some of these electronic wastes. In this study, fungi isolated from soil polluted by EEW were grown on potatoes dextrose agar (PDA) medium. The estimation of the biodegradation was achieved by inoculation of both rechargeable batteries and printed circuit boards on a minimum solid and liquid medium with selected fungal strains. During the process of biodegradation on solid medium, microscopic observation was done, and on liquid medium the production of keratinolytic enzymes was evaluated using a colorimetric method after incubation with keratine powder. After 30 days, the obtained results showed that Geotrichum candidum is capable of degrading battery and circuit boards with rates of 23% and 71%, respectively, while Rhizopus stolonifer reduced battery weight by 7% and printed circuit boards by 60%. Microscopic observations showed no morphological modification in Geotrichum candidum, while there was sporocyst formation in Rhizopus stolonifer. The detection of enzymatic production indicated that there is a relation between the biodegradation process of electronic wastes and keratinolytic enzymes in Geotrichum candidum.

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The Combination of Carbon Source and the Addition of Phenylacetic Acid (PAA) to Growth Medium Penicillium chrysogenum to Enhance of Penicillin (Pen G) Production

et al. 2020

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The nutrition factor governs the growth and production of Penicillin G (Pen G) by Penicillium chrysogenum in a broth medium. Proper nutrition can improve Pen G antibiotic production. In this research, the optimal condition for Pen G production from P. chrysogenum in a standard culture medium and various carbon sources medium (glucose, lactose, maltose, and sucrose) were done for ten days. Phenylacetic Acid (PAA) precursor at 0.0 – 0.6 gL-1 (increment 0.1) was used to improve Pen G production. The Pen G was detected by HPLC, compared with the standard (Penicillin G Sodium Salt). The results showed that the PDB standard medium and lactose medium (150 rpm, at 30°C) produced 0.425 gL-1 and 0.107 gL-1 Pen G. Addition of 0.6 gL-1 PAA improved the Pen G production up to 0.045 gL-1 in the PDB medium, become the final concentration of 0.470 gL-1 and 2.460 gL-1 in the lactose medium, become the final concentration of 2.565 gL-1. The antibiotic’s activity against the pathogenic bacteria, i.e., B. subtilis, S. aureus, and S. typhi employing the disk diffusion method, has been done. The TLC method’s detection of the potential Pen G spots was conducted with ethyl acetate: distilled water: acetic acid (60:20:20) as the mobile phase. The Pen G extracts could inhibit the growth of all tested bacteria in Rf 0.65. This study informs the proper combination of carbon source and precursor effects and increases the bioproduction of Pen G from P. chrysogenum.

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Effect of Zinc Metal at High Concnetration on Secondary Metabolic Pathways in Penicillium Chrysogenum Strain

Cited by 3 publications

References 11 publications

Taxonomic and functional analysis of soil microbial communities in a mining site across a metal(loid) contamination gradient

Taxonomic and functional analysis of soil microbial communities in a mining site across a metal(loid) contamination gradient

In Vitro Removal of Electronic and Electrical Wastes by Fungi Isolated from Soil at Annaba Area in Northeast of Algeria

The Combination of Carbon Source and the Addition of Phenylacetic Acid (PAA) to Growth Medium Penicillium chrysogenum to Enhance of Penicillin (Pen G) Production

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