Effect of yeast with bacteriocin from rumen bacteria on growth performance, caecal flora, caecal fermentation and immunity function of broiler chicks

Abstract

The aim of the current study was to investigate the effects of bacteriocin of Ruminococcus albus 7 that is expressed by yeast on growth performance, caecal flora, caecal fermentation and immunity function of broilers. A total of 180, one-day-old healthy broiler chicks were randomly divided into three groups: control, bacteriocin (2·5 g/kg feed) and nosiheptide (NHT) (2·5 mg/kg, as antibiotic control). Growth performance, caecal flora, caecal fermentation products and immunoglobulin (Ig) concentration were determined when chicks were 21 and 35 days old. The gene expression of avian β-defensin (AvBD) and mucin (MUC2) were measured at 35 days old. The supplementation of bacteriocin and NHT had no significant effect on body weight gain (BWG) during the experimental period. Bacteriocin supplementation significantly enhanced the growth of Lactobacillus (P < 0·05) and resulted in higher lactate concentration (P < 0·01) in broiler caeca at 21 days old. Both bacteriocin and NHT supplementation resulted in lower Escherichia coil (P = 0·072) and Enterococcus (P = 0·038) counts than in the control group at 35 days old. The bacteriocin treatment group tended to increase bile IgA and showed higher bile IgA than the NHT treatment group (P = 0·059) at 35 days old. Higher levels of AvBD1, AvBD4 and MUC2 gene expression were observed in the NHT treatment group (P < 0·05), but expression of AvBD9 was significantly decreased in both bacteriocin and NHT treatment groups (P < 0·05). In conclusion, bacteriocin supplementation elevated the caecal Lactobacillus counts and affected the immunity function. These benefits suggested that bacteriocin supplementation could be a potential alternative to antibiotics in broiler feed.

Bacterial community composition in Adélie (Pygoscelis adeliae) and Chinstrap (Pygoscelis antarctica) Penguin stomach contents from Signy Island, South Orkney Islands

“…Based on a range of studies that have focused on poultry and captive birds, avian gut microbiota are known to benefit their host bird"s health, growth and ultimately reproductive success, mainly by degrading and converting consumed food to nutrients thereby providing energy to the host (Robrish et al 1991;Chen et al 2002;Bjerrum et al 2006;Stanley et al 2012;Roggenbuck et al 2014), and by excreting antibiotics against pathogens (Portrait et al 2000;Van Der Wielen et al 2000;Chen et al 2013). Although phylogenetic factors may also play a role (Grond et al 2014;Waite and Taylor 2014), the environment has been claimed to exert a strong influence on avian gut microbiota, with factors such as bird diet and habitat being important (Lucas and Heeb 2005;Maul et al 2005;Hammons et al 2010;Hird et al 2014;Roggenbuck et al 2014).…”

Effect of yeast with bacteriocin from rumen bacteria on laying performance, blood biochemistry, faecal microbiota and egg quality of laying hens

“…This finding suggested that the higher lactobacilli concentration in the caeca of the bacteriocin supplementation group could result from the effect of bacteriocin expression in yeast cells. The analysis of microbiota composition of broiler chicks indicated that the caecal bacterial population of the yeast with bacteriocin supplementation group was probably influenced by the synthetic effects of bacteriocin and yeast (Chen et al., ).…”

Albusin B, mass‐produced by the Saccharomyces cerevisiae suppression system, enhances lipid utilisation and antioxidant capacity in mice

“…It is sensitive to heat but is unaffected by mannose . Moreover, albusin B has been shown to inhibit the growth of Gram‐positive bacteria and is considered an alternative to antibiotics in animal feed . In our previous study, albusin B was mass‐produced by a yeast system and supplemented to broilers for 5 weeks .…”

“…Evidence showed that albusin B modulated the gut flora by inhibiting pathogenic bacteria and elevating lactobacilli counts . It increased intestinal nutrient absorption, improved gut barrier functions and provided passive immunoprotection against invading pathogens in the gastrointestinal tract . Furthermore, albusin B increased hepatic fatty acid (FA) utilisation and enhanced systemic antioxidant capacity .…”

“…Furthermore, albusin B increased hepatic fatty acid (FA) utilisation and enhanced systemic antioxidant capacity . These effects contributed to the increase in growth performance of broiler chickens …”