Effect of Xihuang Pill on microcirculation in DMBA combined estrogen and progesterone induced breast precancerous lesions rats

Li, Dehui; Su, Yifan; Fan, Huanfang; Sun, Cheng-Yi; Han, Changhui; Packham, MA; Yan, Jiaojiao

doi:10.1088/1755-1315/474/5/052053

Cited by 5 publications

(9 citation statements)

References 5 publications

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“…Its mechanisms might be associated with regulating the protein and gene expression of Cyclin A, CDK2, Bcl-2, Bax, mTOR and VEGF [8,19,20]. Based on our research [8][9][10]17], we hypothesized that XHP pharmacological mechanism for inhibiting the development of breast precancerous lesions could be closely interrelated to the PI3K/Akt/mTOR signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

“…We have established the rat model of breast precancerous lesion as previously reported on the literature [9][10][11]. On the 1st day, DMBA dissolved in sesame oil was given to rats by gavage at the ratio of 1 ml•100g − 1 .…”

Section: Animal Model Establishmentmentioning

confidence: 99%

“…Through clinical practice, it can be used to cure breast precancerous lesions and prevent breast cancer with distinct curative effect [6,7]. Our prophase research found that [8][9][10] XHP inhibited the activity of breast precancerous lesions MCF-10AT cells and induce it apoptosis by suppressing mTOR and VEGF protein expression of PI3K/AKT/mTOR signal pathway, and improve breast microcirculation and abnormal hemorheology in 7,12-dimethylbenzoanthracene (DMBA) combined estrogen and progesterone induced breast precancerous lesions rats. Breaking the balance between cell proliferation and apoptosis is the most important mechanism of breast cancer progression.…”

Section: Introductionmentioning

confidence: 99%

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Xihuang Pill inhibits the development of DMBA combined estrogen and progesterone induced breast precancerous lesions rats by PI3K/AKT/mTOR signaling pathway

Fan

Guo

et al. 2021

Preprint

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Objective. To study the inhibitory effect of Xihuang Pill on the development of DMBA combined estrogen and progesterone induced breast precancerous lesions rats by PI3K/AKT/mTOR signaling pathway, and to explore the effect of Xihuang Pill in preventing and treating breast cancer. Method. Establishment of a rat model of breast precancerous lesion with DMBA combined estrogen and progesterone sequential induction for 10 weeks. Xihuang Pill was administered by gavage continuously for 4 weeks. Take rat breast tissue and stain with hematoxylin- eosin (HE). The pathomorphological changes were observed with light microscope; TUNEL staining to detect cell apoptosis in breast tissue; Western blot was used to detect the protein expression of P-PI3K, P-AKT (S473), P-AKT (T308), PTEN, P-Tuberin/TSC2, P-Tuberin (p-S939), p-mTOR, P-4E-BP1 in breast tissues. The qRT-PCR was used to detect the gene expression of PTEN mRNA and VEGF mRNA. Immunohistochemistry was used to detect the protein expression of P-S6, p-p70s6k and VEGF. Result. Compared with the disease model group, the low, middle and high dose Xihuang Pill groups could significantly reduce the degree of breast pathology, and the number of apoptosis of breast precancerous lesions cells increased with the increase of Xihuang Pill dose; The expression levels of P-PI3K, P-AKT (S473), P-AKT (T308), p-mTOR, P-4E-BP1, p-S6, p-p70S6K, VEGF protein and VEGF mRNA dropped with the increase of Xihuang Pill dose. The expression levels of PTEN, P-Tuberin/TSC2, P-Tuberin (p-S939) protein and PTEN mRNA elevated with the increase of Xihuang Pill dose. Conclusion. Xihuang Pill can promote the apoptosis of breast precancerous lesion cells and reduce the proliferation of vascular endothelial cells, and then inhibit the progression of breast precancerous lesions. Its mechanism probably associated with the regulation of PI3K/AKT/mTOR pathway related gene protein expression.

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Section: Discussionmentioning

confidence: 99%

Section: Animal Model Establishmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Xihuang Pill inhibits the development of DMBA combined estrogen and progesterone induced breast precancerous lesions rats by PI3K/AKT/mTOR signaling pathway

Fan

Guo

et al. 2021

Preprint

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“…We established a rat model of precancerous breast lesions as previously reported in the literature [9][10][11].…”

Section: Animal Model Establishmentmentioning

confidence: 99%

Xihuang Pill Inhibits The Development of DMBA Combined With Oestrogen- And Progesterone-Induced Precancerous Breast lesions In Rats By The PI3K/AKT/mTOR Signaling Pathway

Li¹,

Fan²,

Guo³

et al. 2021

Preprint

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Background:To study the inhibitory effect of Xihuang pill on the development of DMBA combined with oestrogen- and progesterone-induced breast precancerous lesions in rats by the PI3K/AKT/mTOR signaling pathway and to explore the effect of Xihuang pill in preventing and treating breast cancer. Method: Establishment of a rat model of precancerous breast lesions with DMBA combined with oestrogen and progesterone sequential induction for 10 weeks. Xihuang pill was administered continuously by gavage for 4 weeks. Rat breast tissue was stained with haematoxylin-eosin (HE). The pathomorphological changes were observed with a light microscope. TUNEL staining was used to detect cell apoptosis in breast tissue. Western blotting was used to detect the protein expression of P-PI3K, P-AKT (S473), P-AKT (T308), PTEN, P-tuberin/TSC2, P-tuberin (p-S939), p-mTOR, and P-4E-BP1 in breast tissues. qRT-PCR was used to detect the gene expression of PTEN mRNA and VEGF mRNA. Immunohistochemistry was used to detect the protein expression of P-S6, p-p70s6k and VEGF.Result:Compared with the disease model group, the low-, middle- and high-dose Xihuang pill groups could significantly reduce the degree of breast pathology, and the number of apoptotic precancerous breast lesion cells increased with increasing Xihuang pill dose. The expression levels of P-PI3K, P-AKT (S473), P-AKT (T308), p-mTOR, P-4E-BP1, p-S6, p-p70S6K, VEGF protein and VEGF mRNA dropped with increasing Xihuang pill dose. The expression levels of PTEN, P-tuberin/TSC2, P-tuberin (p-S939) protein and PTEN mRNA increased with increasing Xihuang pill dose. Conclusion:Xihuang pill can promote the apoptosis of precancerous breast lesion cells, reduce the proliferation of vascular endothelial cells, and then inhibit the progression of precancerous breast lesions. Its mechanism is probably associated with the regulation of the PI3K/AKT/mTOR pathway related protein expression.

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“…It is a classic prescription for breast precancerous lesions and prophylaxis and treatment of breast cancer, with definite clinical effects. 9,10 Our previous studies have shown that, [11][12][13] XHP can inhibit the activity of MCF-10AT cells in precancerous breast lesions and induce apoptosis by inhibiting the expression of mTOR and VEGF in PI3K/ AKT/mTOR signaling pathway, and improve the microcirculation and hemorheology of breast precancerous lesions induced by 7,12-dimethylbenzanthracene (DMBA) combined with estrogen and progesterone. Modern studies have shown that immune function status corresponds to the occurrence and progression of breast cancer, and cellular immunity plays an essential part of anti-breast cancer immunity.…”

Section: Introductionmentioning

confidence: 99%

Based on BATMAN-TCM to Explore the Molecular Mechanism of Xihuang Pill Regulating Immune Function to Treat Breast Precancerous Lesions

Li¹,

Fan²,

Dong³

et al. 2021

BCTT

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Objective:The specific mechanism of Xihuang Pill in the treatment of breast precancerous lesions and breast cancer has not yet been elucidated. Methods: In our study, BATMAN-TCM (a Bioinformatics Analysis Tool for Molecular mechanisms of Traditional Chinese Medicine) was used to forecast the relationship among chemical components, immune targets, and diseases of each herb in Xihuang Pill and constructed a component-target-disease network. Taking breast precancerous lesion model rats as the research object, the molecular mechanism of Xihuang Pill regulating immunity was analyzed. Results: BATMAN-TCM prediction showed that 309 genes were enriched in the biological process of "immune system response", which was the target of Xihuang Pill to regulate the immune system. The target of breast cancer disease and the genes related to Xihuang Pill's immune system response were crossed, and 88 cross genes were obtained. According to the enrichment results of GO/KEGG pathway, T cell activation was found to be the most relevant. We select Th1 cells (IL-2, IFN-γ) and Th2 cells (IL-4, IL-10) among them for animal experiment verification. The results show that Xihuang Pill can upregulate the serum IFN-γ and IL-2 levels, reduce the IL-4 and IL-10 levels, and regulate the balance of Th1/Th2 cells in the peripheral blood of rats with breast precancerous lesions. Conclusion: Xihuang Pill targets a variety of immune-related molecules related to breast precancerous lesions and is a traditional Chinese medicine formula that effectively regulates immune function.

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Effect of Xihuang Pill on microcirculation in DMBA combined estrogen and progesterone induced breast precancerous lesions rats

Cited by 5 publications

References 5 publications

Xihuang Pill inhibits the development of DMBA combined estrogen and progesterone induced breast precancerous lesions rats by PI3K/AKT/mTOR signaling pathway

Xihuang Pill inhibits the development of DMBA combined estrogen and progesterone induced breast precancerous lesions rats by PI3K/AKT/mTOR signaling pathway

Xihuang Pill Inhibits The Development of DMBA Combined With Oestrogen- And Progesterone-Induced Precancerous Breast lesions In Rats By The PI3K/AKT/mTOR Signaling Pathway

Based on BATMAN-TCM to Explore the Molecular Mechanism of Xihuang Pill Regulating Immune Function to Treat Breast Precancerous Lesions

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