Effect of whey protein supplementation on levels of endocannabinoids and some of metabolic risk factors in obese women on a weight-loss diet: a study protocol for a randomized controlled trial

Haidari, Fatemeh; Aghamohammadi, Vahideh; Mohammadshahi, Majid; Angali, Kambiz Ahmadi

doi:10.1186/s12937-017-0294-x

Cited by 15 publications

(14 citation statements)

References 16 publications

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“…The ECs might also represent a key target for the treatment of abdominal obesity and associated metabolic changes. It seems that ECB is a key player in macronutrient metabolism between organs involved in food intake and systemic energy balance [17]. Among the strengths of the study was the use of a lower dose of whey protein, regular follow-up of subjects for observing diet, supplement intake and physical activity, and assessment of effect of whey protein on endocannabinoid levels for the first time.…”

Section: Discussionmentioning

confidence: 99%

“…Given the putative role of whey protein, plasma EC association with visceral obesity and metabolic profile, and due to the fact that the effect of whey protein on EC concentration is not clear [17], we aimed to evaluate the effects of whey protein supplementation on fasting levels of endocannabinoids, lipid profile, glucose, insulin, HOMA-IR, and anthropometric indices in obese women on a weight-loss diet.…”

Section: Introductionmentioning

confidence: 99%

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Whey protein supplementation reducing fasting levels of anandamide and 2-AG without weight loss in pre-menopausal women with obesity on a weight-loss diet

Haidari

Aghamohammadi

Mohammadshahi

et al. 2020

Trials

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Background: Despite the importance of dairy proteins in modifying of metabolic abnormalities, no attention has been given to their effects on endocannabinoids. Methods: A total number of 60 obese women were recruited in a 2-month randomized clinical trial. Following random allocation, they were assigned to one of the two groups: control (n = 30) and intervention (n = 30). Then, all the subjects followed a hypocaloric diet of 800 kcal below estimated energy needs. The intervention group received isocaloric weight-loss diet and whey protein powders (30 g/day). Baseline and 2-month fasting anthropometric, blood glucose, serum insulin, insulin resistance, lipid profile, AEA, and 2-AG were measured. Results: The study groups were homogenous in terms of baseline characteristics (p > 0.05) except for MUFA intake (p = 0.021). There were no significant differences in energy and macronutrient intakes in the intervention group compared to the control group at the end of the study (p > 0.05). The results of the ANCOVA did not show significant reductions in body weight and BMI of the intervention group compared to the control group (p > 0.05); however, WC, body fat, FBS, AEA, 2-AG, total cholesterol, and triglyceride decreased and HDL-c significantly increased in the intervention group compared to the control group (p < 0.05). Conclusions: In this study, the effects of simultaneous weight-loss diet and whey protein supplementation on the reduction of endocannabinoids were determined. Trial registration: Iranian Registry of Clinical Trials IRCT2017021410181N8. Registered on March 2017.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Whey protein supplementation reducing fasting levels of anandamide and 2-AG without weight loss in pre-menopausal women with obesity on a weight-loss diet

Haidari

Aghamohammadi

Mohammadshahi

et al. 2020

Trials

Self Cite

View full text Add to dashboard Cite

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“…Energy needs were considered by Mifflin Jeor St. equation: BMR (kcal/day) = 10 × Weight (kg) + 6.25 × Height (cm) − 5 × Age (year) − 161 (kcal/day) [ 27 ], and each subject of study in both groups followed a hypocaloric diet of 500 kcal below estimated energy requirements. In both study groups, the proportion of macronutrients were 55%, 30%, 15% for carbohydrate, fat, and protein; respectively [ 28 ]. OLE or placebo capsules were consumed twice daily, one capsule after lunch and one capsule after dinner.…”

Section: Methodsmentioning

confidence: 99%

Olive Leaf Extract Supplementation Combined with Calorie-Restricted Diet on Reducing Body Weight and Fat Mass in Obese Women: Result of a Randomized Control Trial

et al. 2021

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“…Plasma samples were processed no later than 10 min. Analysis of AEA and 2-AG levels was carried out using human enzyme-linked immunosorbent assay (ELISA) kits (Hangzhou Eastbiopharm Co. Ltd., Hangzhou, Zhejiang, China) [20,21].…”

Section: Anthropometric and Biochemical Measurementsmentioning

confidence: 99%

The association of dietary patterns with endocannabinoids levels in overweight and obese women

et al. 2020

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Background: Higher levels of anandamide (AEA) and 2-arachidonoylglycerol (2-AG), the main arachidonic acidderived endocannabinoids, are frequently reported in overweight and obese individuals. Recently, endocannabinoids have become a research interest in obesity area regarding their role in food intake. The relationship between dietary patterns and endocannabinoids is poorly understood; therefore, this study evaluated the association of the dietary patterns with AEA and 2-AG levels in overweight and obese women. Methods: In this cross sectional study, 183 overweight and obese females from Tabriz, Iran who aged between 19 and 50 years old and with mean BMI = 32.44 ± 3.79 kg/m 2 were interviewed. The AEA and 2-AG levels were measured, and the dietary patterns were assessed using food frequency questionnaire. To extract the dietary patterns, factor analysis was applied. The association between AEA and 2-AG levels and dietary patterns was analyzed by linear regression. Results: Three major dietary patterns including "Western", "healthy", and "traditional" were extracted. After adjusting for age, physical activity, BMI, waist circumference, and fat mass, higher levels of AEA and 2-AG were observed in participants who were in the highest quintile of the Western pattern (P < 0.05). Also, in both unadjusted and adjusted models, significantly lower levels of AEA and 2-AG were detected in the women of the highest quintile of the healthy pattern (P < 0.01). Moreover, there was no significant association between "traditional" pattern and AEA and 2-AG levels in both unadjusted and adjusted models (P > 0.05). Conclusion: In regard with the lower levels of endocannabinoids in healthy dietary pattern, adherence to healthy pattern might have promising results in regulating endocannabinoids levels.

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Effect of whey protein supplementation on levels of endocannabinoids and some of metabolic risk factors in obese women on a weight-loss diet: a study protocol for a randomized controlled trial

Cited by 15 publications

References 16 publications

Whey protein supplementation reducing fasting levels of anandamide and 2-AG without weight loss in pre-menopausal women with obesity on a weight-loss diet

Whey protein supplementation reducing fasting levels of anandamide and 2-AG without weight loss in pre-menopausal women with obesity on a weight-loss diet

Olive Leaf Extract Supplementation Combined with Calorie-Restricted Diet on Reducing Body Weight and Fat Mass in Obese Women: Result of a Randomized Control Trial

The association of dietary patterns with endocannabinoids levels in overweight and obese women

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