Effect of water-soluble β-cyclodextrin polymer on the lipophilicity of polymyxine examined by reversed-phase thin-layer chromatography

Cserháti, Tibor; Bordás, Barna; Fenyvesi, Éva; Szejtli, J.

doi:10.1016/s0021-9673(01)87983-8

Cited by 51 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polygram UVlS4 (Macherey-Nagel, Durren, Germany) (support I), DC-Aluminiumoxide Fzsj (Merck, Darmstadt, Germany) (support 11) and DC-Cellulose F (Merck, Darmstadt, Germany) (support 111) plates were impregnated by overnight predcvelopment in n-hexane-paraffin oil p5 : 5 (vlv) as previously described (Cserhati et al, 1983b). Unimpregnated cellulose plates (support 1V) were also included in the experiments.…”

Section: Methodsmentioning

confidence: 99%

Effect of support characteristics on the reversed‐phase retention behaviour of a non‐homologue series of anticancer drugs

Forgács¹,

Holló²

1994

Biomedical Chromatography

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Effect of support characteristics on the reversed‐phase retention behaviour of a non‐homologue series of anticancer drugs

Forgács¹,

Holló²

1994

Biomedical Chromatography

View full text Add to dashboard Cite

“…Next, the effect of different stationary phases and the presence of organic additives on the performance of the urea-solubilized d-CD mobile phases in TLC was briefly examined. Most work employing CD mobile phases in TLC to date have utilized either reversed-phase or polyamide stationary phases (18)(19)(20)(21)(22)(23)(24)(25)(26). As shown in Table I, polyamide proved useful for the separation of some positional ortho, meta, and para isomers.…”

Section: Effect Of CD Concentration Upon the Selectivitymentioning

confidence: 99%

“…3 Present address: BASF Corp., 1701 Westinghouse Blvd., Charlotte, NC 28241. (HPLC) separations (9-17), there have been relatively few applications involving thin-layer chromatography (TLC). In fact, reversed-phase (RP) TLC has been almost exclusively employed to study the CD inclusion complexes formed by derivatives of a variety of organic compounds (18)(19)(20)(21)(22)(23). In all but one of those reports, polymeric 3-CD was utilized as the mobile-phase additive.…”

Section: Introductionmentioning

confidence: 99%

Thin-layer chromatography with urea solubilized .beta.-cyclodextrin mobile phases

Hinze¹,

Pharr²,

Fu³

et al. 1989

Anal. Chem.

View full text Add to dashboard Cite

Aqueous solutions of urea-solublllzed /8-cydodextrln (ß-CD) are characterized and evaluated as a novel planar chromatographic mobile phase. The resolution of Isomeric ortho-, meta-, and para-dlsubstltuted benzenes as well as that of pesticide, polycyclic aromatic hydrocarbon, and drug test mixes on a polyamide stationary phase demonstrates the viability of this urea-solublllzed ß-CD medium as a thin-layer chromatographic (TLC) mobile phase. In addition, some chromatographic data was obtained on reversed-phase C-12 and C-18 stationary phases. The pertinent chromatographic parameters were determined and contrasted to those previously obtained with CD mobile phases. The Armstrong pseudophase theory was applied and found to adequately describe the retention behavior exhibited by the test solutes as a function of /8-CD concentration In the mobile phase. The effect of urea concentration and presence of alcohol additives on retention and separation was also assessed. Lastly, a brief prospectus on the usefulness of solubilized ß-CD mobile phases In liquid chromatographic separations Is presented.

show abstract

“…This will be particularly rewarding with PCD catalyzed or steered reactions, because of the low solubility of the unsubstituted PCD. Characteristic properties of silver-halogenide containing photographic emulsions, photofilms were improved by admixing ionic soluble PCD-polymers [21,221 Inclusion complexation of lipophilic guest molecules by soluble pCD-polymer showed to be useful in reversed phase thin-layer chromatography (RPTLC) of triphenylmethane derivatives [23], polymyxine [24], and triazines [25]. Solubility of drugs (e. g. indomethacin) in soluble PCDpolymer solutions [16], or in vitro dissolution profiles from solid indomethacin-P CD-polymer preparation were significantly improved [26].…”

Section: Soluble Cd-polymersmentioning

confidence: 99%

Highly Soluble β‐Cyclodextrin Derivatives

Szejtli¹

1984

Starch Stärke

View full text Add to dashboard Cite

P-Cyclodextrin is an inclusion complexing agent produced on industrial scale. The low solubility ofits complexes is a serious disadvantage in many cases. Substituted P-Cyclodextrin derivatives are characterized by much higher solubility. Among methylated derivatives the heptakis-di-0-methyl-P-Cyclodextrin deserves special attention.In its aqueous solutions many poorly soluble or practically insoluble compounds can be dissolved, whereas the solubility enhancement factor is between 3 and 1 200 depending on the substance. Aqueous injectable solutions can be prepared from highly lipophilic compounds, enzymatic reaction rate of lipophilic substrates can be strongly accelerated, biological absorption of drugs or fat-soluble vitamins can be enhanced and vaccine production can be augmented up to 100-fold. Methylated cyclodextrins are able to act as solvents by forming watersoluble inclusion complexes and as phase-transfer catalysts at free radical polymerization. Low molecular cyclodextrin-polymers with molecular weight between 2 000 and 10 000 behave similarly. They are very well soluble, retaining their complexing ability. On account of the macromolecular character the diffusion of dissolved and complexed substances is considerably decelerated. By including into a chiral cavity the entrapped molecules become optically active oriented, and therefore their reactions are sterically controlled.Hochlasliche fl-Cyclodextrin-Derivate. Die industriell hergestellten j3-Cyclodextrine stellen gute Komplexbildner dar, doch hat sich die geringe Loslichkeit ihrer Komplexe in zahlreichen Fallen als nachteilig erwiesen. Im Gegensatz dazu sind substituierte P-Cyclodextrin-Derivate vie1 besser loslich. Unter den methylierten Derivaten ist das Heptakis-2,6-di-O-methyl-~-cyclodextrin von besonderem Interesse. In seinen wa0rigen Losungen konnen sehr schwer losliche oder sogar praktisch unlosliche Verbindungen aufgelost werden, wobei sich der Faktor der Loslichkeitszunahme in Abhangigkeit von der chemischen Struktur und der Komplexierbarkeit der Substanz zwischen 3 und 1200 bewegt. Wanrige, injizierbare Losungen konnen aus stark lipophilen Verbindungen hergestellt, enzymatische Reaktionen derartiger Verbindungen erheblich beschleunigt und die biologische Absorption von Arzneimitteln und fettloslichen Vitaminen verbessert sowie die Produktion von Vaccinen bis urn das 100fache erhoht werden. Durch Bildung wasserloslicher Einschluherbindungen wirken Cyclodextrine als Losungsmittel sowie bei frei radikaler Polymerisation als Phasentransfer-Katalysator. Niedermolekulare Cyclodextrin-Polymere rnit Molekulargewichten zwischen 2000 und 10000 verhalten sich ahnlich. Als Folge der relativ hohen Molekularmasse wird die Diffusion der aufgelosten und komplexierten Substanzen erheblich reduziert. Die in den chiralen Hohlraum eingeschlossenen, ansonsten achiralen Verbindungen werden optisch aktiv orientiert, weshalb auch ihre Reaktionen sterisch gesteuert werden.

show abstract

Effect of water-soluble β-cyclodextrin polymer on the lipophilicity of polymyxine examined by reversed-phase thin-layer chromatography

Cited by 51 publications

References 8 publications

Effect of support characteristics on the reversed‐phase retention behaviour of a non‐homologue series of anticancer drugs

Effect of support characteristics on the reversed‐phase retention behaviour of a non‐homologue series of anticancer drugs

Thin-layer chromatography with urea solubilized .beta.-cyclodextrin mobile phases

Highly Soluble β‐Cyclodextrin Derivatives

Contact Info

Product

Resources

About