Abstract:Background: Many years have been devoted to preserve fertility, but the effect of cryopreservation on gene functionality in primary, secondary and tertiary follicular stages; is still unclear. The present study was designed to assess the effect of vitrification on the histological structures and expression of follicular cells related genes.
Methods: The buffalo ovarian cortical tissues were vitrified in two-steps. The sliced cortical tissues were incubated with VS1 (8.5% DMSO and 8.5% EG) for 10 min and se… Show more
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