Effect of Vitamins A, E and C on Liver Enzyme Activity in Rats Exposed to Organophosphate Pesticide Diazinon

Abstract

Diazinon, a commonly used organophosphorus pesticide, has been widely used throughout the world in agriculture and horticulture to control insects that feed on crops, ornamentals, lawns, fruits, vegetables and other food products. The toxicity of the DZN causes adverse effects on many organs. The purpose of this study was to examine the protective effect of vitamins A, E and C on liver enzymes alanine transaminase (ALT), Aspartate aminotransferase (AST) and Lactate Dehydrogenase (LDH) in rats exposed to diazinon. In this study, male wistar rats were randomly divided into 10 different groups. The groups were administered normal saline, soybean oil (as the solvent for diazinon and fat-soluble vitamins), diazinon, (30 mg kg(-1), vitamins E, C and A (100, 500 mg kg(-1) and 400 IU kg(-1), respectively) and a combination of diazinon with the same dose of each vitamin intraperitoneally i.p.daily for 14 days. Seven days after the final injection, the animals were anesthetized and blood samples were taken. The photometric method was used to measure the activity of the enzymes. The activities of ALT and AST in the diazinon group were significantly higher than that observed in the control group; however, the diazinon/vitamin E, A, C group displayed significant reduction in ALT and AST activities compared to the diazinon group. The lowest level of LDH enzyme activity was observed in the dazinon/vitamin C group and this was statistically lower than the diazinon group. The results of this study revealed that vitamin E, A and C have a potent protective effect against diazinon-induced hepatotoxicity in rats, which may be due to the scavenging of free radicals and increased antioxidant status.

Antioxidant effect of vitamin E on diphenylamine-induced hepato-renal oxidative stress and structural changes in rat fetuses

“…Also, Shokrzadeh et al (2012) revealed that Vit. E, A, and C have a protective effect against diazinon-induced hepatotoxicity in rats, as it increased the antioxidant status.…”

In vivo study of dose-dependent antioxidant efficacy of functionalized core–shell yttrium oxide nanoparticles

“…Nanomaterials have recently attracted a lot of attention for their potential application as nanomedicines. Antioxidants such as vitamins, trace minerals, and medicines have all been widely used to combat oxidative stress (Shokrzadeh et al 2012;Lasram et al 2014) but with a lot of limitations. Scientific contributions to this research area reveal that some NPs have a free radical scavenging activity and hence have proved effective in oxidative stress conditions (Caputo et al 2014;Karakoti et al 2012).…”

Investigation of the effects of some water-soluble vitamins on glutathionereductase enzyme purified from bovine liver

“…In some studies similar to ours, the effects of vitamins E, A, and C on rats with diazinon were investigated and the levels of AST and ALT decreased after these compounds were administered. According to these results, the vitamins protected against liver effects of diazinon and also may have radical cleansing and antioxidant properties [37]. In another study, the effect of vitamin E (600 mg) on sick and normal groups was investigated for 6 days.…”