Effect of vitamin E supplementation on arsenic induced alteration in blood biochemical profile, oxidant/antioxidant status, serum cortisol level and retention of arsenic and selenium in goats

Mohanta, Ranjan Kumar; Garg, Anil Kumar; Dass, Ram

doi:10.1016/j.jtemb.2014.08.013

Cited by 15 publications

(14 citation statements)

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“…The metabolism and excretion of As is dependent on GSH. In liver, As is metabolized into soluble mono-, di- or tri hydroxyl arsenic acid in presence of GSH [34]. As has strong affinity toward thiol group resulting depletion of GSH level in the tissues of NaAsO 2 -intoxicated mice confirmed this fact.…”

Section: Discussionmentioning

confidence: 92%

“…The increased concentrations of tissue specific enzymes in serum are the indications of membrane leakage. LDH and CK are the intracellular enzymes having high tissue specificity [31,34]. Increased levels of serum LAD and CK in NaAsO 2 (10 μM) intoxicated mice supported the membrane damage and concomitant reduction of cell viability.…”

Section: Discussionmentioning

confidence: 99%

“…Increased levels of serum LAD and CK in NaAsO 2 (10 μM) intoxicated mice supported the membrane damage and concomitant reduction of cell viability. As undergoes biotransformation in the liver where it binds with the thiol group of proteins and enzymes, thereby, interferes the integrity of plasma membrane of hepatocytes leading to leakage of AST and ALT in serum [34,35]. As-intoxication significantly increased serum level of urea, which may be indicative of protein catabolism and renal dysfunction [31].…”

Section: Discussionmentioning

confidence: 99%

“…GST protects cells against oxidants by removing free radicals. Significant reduction of these antioxidant enzymes during As-intoxication may be due to an enhanced production of ROS and/or down-regulation in the synthesis of antioxidant enzymes by persistent toxic effect of NaAsO 2 [34]. Thiol-based antioxidant system, GSH, helps to protect cells from ROS [3].…”

Section: Discussionmentioning

confidence: 99%

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Ameliorative effect of water spinach, Ipomea aquatica (Convolvulaceae), against experimentally induced arsenic toxicity

et al. 2015

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BackgroundIpomea aquatica (Convolvulaceae) is traditionally used against Arsenic (As) poisoning in folk medicines in India. The present study was designed to explore the therapeutic role of aqueous extract of I. aquatica (AEIA) against As-intoxication.MethodsAEIA was chemically standardized by spectroscopic and chromatographic analysis. The cytoprotective role of AEIA was measured on isolated murine hepatocytes. The effect on redox status were measured after incubating the hepatocytes with NaAsO2 (10 μM) + AEIA (400 μg/ml). The protective effect of AEIA (400 μg/ml) in expressions of apoptotic proteins were estimated in vitro. The protective role of AEIA was measured by in vivo assay in mice. Haematological, biochemical, As bioaccumulation and histological parameters were evaluated to ensure the protective role of AEIA (100 mg/kg) against NaAsO2 (10 mg/kg) intoxication.ResultsPhytochemical analysis revealed presence of substantial quantities of phenolics, flavonoids, saponins and ascorbic acid in AEIA. Incubation of murine hepatocytes with AEIA (0–400 μg/ml) + NaAsO2 (10 μM) exerted a concentration dependent cytoprotective effect. Incubation of murine hepatocytes with NaAsO2 (10 μM, ~ IC50) induced apoptosis via augmenting oxidative stress. NaAsO2 treated hepatocytes exhibited significantly (p < 0.01) enhanced levels of ROS production, lipid peroxidation and protein carbonylation with concomitant depletion of antioxidant enzymes (p < 0.05-0.01) and GSH (p < 0.01) levels. However, AEIA (400 μg/ml) + NaAsO2 (10 μM) could significantly (p < 0.05-0.01) reinstate the aforementioned parameters to near-normal status. Besides, AEIA (400 μg/ml) could significantly counteract (p <0.05-0.01) ROS mediated alteration in the expressions of apoptotic proteins viz. Bcl-2, BAD, Cyt C, Apaf 1, caspases, Fas and Bid. In in vivo bioassay, NaAsO2 (10 mg/kg) treatment in mice caused significantly (p < 0.05-0.01) elevated As bioaccumulation, ATP levels, DNA fragmentations and oxidative stress in the liver, kidney, heart, brain and testes along with alteration in cytoarchitecture of these organs. In addition, the serum biochemical and haematological parameters were significantly (p < 0.05-0.01) altered in the NaAsO2-treated animals. However, concurrent administration of AEIA (100 mg/ml) could significantly reinstate the NaAsO2-induced pathogenesis.ConclusionPresence of substantial quantities of dietary antioxidants within AEIA would be responsible for overall protective effect.Electronic supplementary materialThe online version of this article (doi:10.1186/s12967-015-0430-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Ameliorative effect of water spinach, Ipomea aquatica (Convolvulaceae), against experimentally induced arsenic toxicity

et al. 2015

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“…defensive mechanism against the free radicals and restores the normal functions of tissues against the arsenic toxicity in goats (Mohanta et al, 2015). Keeping in view the rising level of arsenic in drinking water under the climatic conditions of Pakistan, this study was designed to observe the toxic effects Teddy bucks.…”

Section: Research Articlementioning

confidence: 99%

Effects of Vitamin E on Hemato-Biochemical Constituents, Kidney Parenchyma and Tissue Accumulation in Sodium Arsenite Exposed Teddy Goat Bucks

Zubair¹

2018

PVJ

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The objective of this study was to find out the toxicity of sodium arsenite on hematology, serum biochemical constituents and kidney of Teddy bucks. The accumulation of arsenic in blood as well as tissues along its excretion in feces and urine was also measured. Vitamin E was also used to ameliorate the toxicity on these parameters. Adult twelve Teddy bucks were equally divided into three groups; A, B and C. The animals in group A was kept as control, while in groups B and C were fed with sodium arsenite 5 mg/kg and sodium arsenite 5 mg/kg +Vit E 200 mg/kg body weights respectively for twelve weeks. Body weights, blood and serum biochemical parameters were evaluated fortnightly in all the groups. Similarly, total arsenic accumulation in blood and excretion in urine and feces were measured after every two weeks. At the end of experiment, animals were slaughtered and visceral organs were processed for arsenic level and histopathologically studies. On analysis of data, it was proved that there was significant P<0.05 decrease in body weight, total protein, albumin and creatinine in arsenic treated animals. Whereas, total level of arsenic in blood and tissues was increased significantly in arsenic exposed animals. The results also revealed that vitamin E alleviated toxic effects of sodium arsenite on body weight, blood and serum biochemical constituents in restoration of normal values of these parameters. It was also proved that this vitamin increased the arsenic concentration in urine and feces. It was concluded that arsenic causes the toxicity and vitamin E scavenges this toxicity.

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Occupational and Environmental Exposures and Cancers in Developing Countries

Hashim

Boffetta

2014

Annals of Global Health

109

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Effect of vitamin E supplementation on arsenic induced alteration in blood biochemical profile, oxidant/antioxidant status, serum cortisol level and retention of arsenic and selenium in goats

Cited by 15 publications

References 47 publications

Ameliorative effect of water spinach, Ipomea aquatica (Convolvulaceae), against experimentally induced arsenic toxicity

Ameliorative effect of water spinach, Ipomea aquatica (Convolvulaceae), against experimentally induced arsenic toxicity

Effects of Vitamin E on Hemato-Biochemical Constituents, Kidney Parenchyma and Tissue Accumulation in Sodium Arsenite Exposed Teddy Goat Bucks

Occupational and Environmental Exposures and Cancers in Developing Countries

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