Effect of vitamin E supplementation on circulating cell adhesion molecules pre- and post-coronary angioplasty

Abstract

SUMMARY.The soluble adhesion molecules P-selectin (sP-selectin) and intercellular adhesion molecule-1 (sICAM-1) are derived from platelets and endothelial cells. Circulating concentrations of these soluble adhesion molecules are raised in patients with atherosclerosis and following percutaneous transluminal coronary angioplasty (PTCA). We have investigated the effects of vitamin E supplements (800 IU/day) on circulating plasma ICAM-1 and P-selectin levels pre-and post-PTCA. Patients, randomized to group, were pre-treated with vitamin E or placebo (soybean oil) for 1 month before routine PTCA. Plasma sICAM-1 and sP-selectin were measured by enzyme-linked immunosorbent assay on blood taken immediately pre-and post-PTCA. Total protein and a-tocopherol were measured on the same samples. Plasma a-tocopherol concentrations increased in patients receiving vitamin E: 19´1 (1´5) [mean (standard error of the mean, SEM)] mg/mL post-PTCA versus 13´9 (0´6) mg/ mL pre-PTCA (n=23; P50´01). Plasma sP-selectin and sICAM-1 levels were not signi®cantly increased following PTCA in the vitamin E group. Pre-angioplasty mean (SEM) plasma sP-selectin concentration in the vitamin E group was 8´83 (0´97) ng/mg protein; the corresponding mean post-angioplasty value was 9´34 (0´89) ng/mg protein (P=0´85). The mean (SEM) pre-angioplasty sICAM-1 concentration in this group was 2´18 (0´24) ng/mg protein, and was 2´20 (0´23) ng/ mg protein following angioplasty (P=0´84). In the placebo group (n=24) there was a signi®cant increase in mean (SEM) sP-selectin concentration following angioplasty, from 7´48 (0´73) to 9´70 (0´78) ng/mg protein (P50´05). The change (mean, SEM) in plasma sP-selectin concentration following angioplasty was signi®cantly greater for the placebo group [2´22 (0´50) ng/mg protein] than for the group receiving vitamin E [0´50 (0´50) ng/mg protein] (P50´02). This difference remained signi®cant (P50´05) even after adjustment for pre-angioplasty P-selectin concentrations. Mean (SEM) plasma sICAM-1 concentrations remained unchanged following angioplasty [preangioplasty: 2´16 (0´20) ng/mg protein; post-angioplasty: 1´97 (0´13) ng/mg protein]. Vitamin E may therefore limit platelet or endothelial activation during PTCA.Vitamin E is a potent, lipid-soluble plasma antioxidant. Taking supplements of vitamin E has been shown to be associated with reduced risk of coronary heart disease in men and women.

Influence of vitamin E on the antiplatelet effect of acetylsalicylic acid in human blood

“…However, our results show that the effect is potentiated by combining acetylsalicylic acid and vitamin E when aggregation is induced by ADP or collagen in whole blood or PRP. This finding confirms clinical observations by several authors who postulated that the association of these two substances would prevent thrombotic events more effectively [13,27]. Moreover, these results are compatible with previous findings in vitro that the effect was potentiated in PRP when collagen was used to induce platelet aggregation [12].…”

Changes in serum concentration of antioxidants following treadmill exercise testing in patients with suspected ischaemic heart disease

“…Urea and electrolyte results were in the normal range for all subjects. Baseline concentrations of plasma vitamin E were approximately 25% lower than for a group of Caucasian subjects with chest pain, and of comparable age (Ferns et al . 2000b), however, a greater proportion of subjects in the present study were current smokers, or diabetic.…”

Department of Health Research Initiative on Nutrition Phase 2: overview of findings and recommendations

“…Publications in the International Journal of Experimental Pathology and in the Annals of Clinical Biochemistry (Ferns et al, 2000).…”