Effect of vitamin E on plasma malondialdehyde, antioxidant enzyme levels and the rates of wound closures during wound healing in normal and diabetic rats

Abstract

Vitamin E is composed of various subfamilies that include tocopherols and tocotrienols. These compounds have antioxidant properties but differ in structure, dietary source and potency. In this study we evaluated the efficacy of alpha-tocopherol as an antioxidant and its role in wound closure in normal and streptozotocin-induced diabetic rats. The healing of 6 cm linear incisions created on the back of each male Sprague-Dawley rat (250-300 g) was monitored by measuring the length of the wounds daily. The rats were divided into two categories; normal and streptozotocin-induced diabetic rats. For each category, the animals were further divided into two groups; those untreated and those receiving 200 mg/kg bodyweight alpha-tocopherols daily by oral gavage. All rats were fed standard food and water ad libitum. Blood samples were taken at 0, 5 and 10 days after the wounds were created for the determination of malondialdehyde levels and red cell superoxide dismutase, catalase and glutathione peroxidase activities. The results showed that alpha-tocopherol reduced plasma malondialdehyde levels, increased glutathione peroxidase activity and accelerated the rate of wound closure in treated rats.

Evaluation of lipid profile and oxidative stress in STZ-induced rats treated with antioxidant vitamin

“…In the present study vitamin E attenuated the increase in lipid hydroperoxide, accompanied by a concomitant increase in the activities of GSH-Px and SOD, which modulated the concentration of ROS. These data were consistent with studies reporting that vitamin E exerted a beneficial effect on the antioxidant enzyme activity in type 1 diabetes (Varvarovská et al 2004;Musalmah et al 2002). Indeed, antioxidant therapy could prevent a disturbance in the mechanism of protection against the deleterious cellular and biomolecular effects that led to alterations in the cell function.…”

Effects of Maternal and Progeny Dietary Vitamin Regimens on the Performance of Ducklings

“…Of particular interest is the increased SOD level observed in ducklings from the maternal high-vitamin premix group. The effects of dietary vitamins on antioxidant enzyme activities remain controversial (Musalmah et al, 2002;Beytut and Aksakal, 2003).…”

Effect of Co-Administration of Vitamin E Isoforms d-<i>α-Tocopherol</i> and d-δ-<i>Tocotrienol Rich Fraction</i> on the Healing of Skin Wounds in Diabetic Rats

“…And thus oxidative stress induces cellular dysfunction and retards angiogenesis and the healing process [21]. Many studies [13,14,[22][23][24] have concluded that the antioxidants help to maintain the oxidant/antioxidant balance, thereby improve the healing of wounds in diabetes. Vitamin E is a well-known antioxidant; its α-tocopherol isoform has scavenging effect on reactive oxygen species (ROS) and stabilizing effect on damaged cell membrane [25,26].…”