Effect of vitamin D supplementation on selected inflammatory biomarkers in older adults: a secondary analysis of data from a randomised, placebo-controlled trial

Abstract

Observational studies have suggested that 25-hydroxyvitamin D (25(OH)D) levels are associated with inflammatory markers. Most trials reporting significant associations between vitamin D intake and inflammatory markers used specific patient groups. Thus, we aimed to determine the effect of supplementary vitamin D using secondary data from a population-based, randomised, placebo-controlled, doubleblind trial (Pilot D-Health trial 2010/0423). Participants were 60-to 84-year-old residents of one of the four eastern states of Australia. They were randomly selected from the electoral roll and were randomised to one of three trial arms: placebo (n 214), 750 μg (n 215) or 1500 μg (n 215) vitamin D 3 , each taken once per month for 12 months. Post-intervention blood samples for the analysis of C-reactive protein (CRP), IL-6, IL-10, leptin and adiponectin levels were available for 613 participants. Associations between intervention group and biomarker levels were evaluated using quantile regression. There were no statistically significant differences in distributions of CRP, leptin, adiponectin, leptin: adiponectin ratio or IL-10 levels between the placebo group and either supplemented group. The 75th percentile IL-6 level was 2·8 pg/ml higher (95 % CI 0·4, 5·8 pg/ml) in the 1500 μg group than in the placebo group (75th percentiles:11·0 v. 8·2 pg/ml), with a somewhat smaller, non-significant difference in 75th percentiles between the 750 μg and placebo groups. Despite large differences in serum 25(OH)D levels between the three groups after 12 months of supplementation, we found little evidence of an effect of vitamin D supplementation on cytokine or adipokine levels, with the possible exception of IL-6.

High dose vitamin D supplementation can improve menstrual problems, dysmenorrhea, and premenstrual syndrome in adolescents

“…Taking 50000 IU-vitamin D for 8 weeks has been recommended for the treatment of vitamin D deficiency [26]. Similar results were observed for the effect of vitamin D supplementation on serum 25 OH) vitamin D in previous studies [41][42][43].…”

A multi-ingredient nutritional supplement enhances exercise training-related reductions in markers of systemic inflammation in healthy older men

“…Exercise training (Singh and Newman 2011), and certain isolated nutritional supplements such as whey protein (de Aguilar<Nascimento et al 2011;Sugawara et al 2012), creatine (Santos et al 2004;Bassit et al 2008), vitamin D/calcium (Bjorkman et al 2009;Waterhouse et al 2015), and omega<3 polyunsaturated fatty acids (n<3 PUFA) (Zhao et al 2009;Tartibian et al 2011), have previously been shown to reduce circulating concentrations of pro<inflammatory cytokines. Recently we demonstrated that a broad spectrum nutritional supplement (which included these aforementioned ingredients: creatine, whey, vitamin D, calcium, and n<3 PUFA) improved strength and lean tissue mass in a group of healthy older men versus a nutrition control (Bell et al 2017).…”

“…Low<grade sterile inflammation due to increased oxidative stress (Singh and Newman 2011) and/or central (particularly visceral) adiposity (Ryan 2000;Singh and Newman 2011) has been suggested as a mechanism by which cardiometabolic disease develops in older age, and is therefore a worthwhile target for intervention. Previous studies have investigated the independent ability of whey protein (de Aguilar<Nascimento et al 2011;Sugawara et al 2012), creatine (Santos et al 2004;Bassit et al 2008), vitamin D (Bjorkman et al 2009;Waterhouse et al 2015), and n<3…”

Vitamin D and inflammatory markers: cross-sectional analyses using data from the English Longitudinal Study of Ageing (ELSA)

“…Evidence from an interventional study investigating the effect of vitamin D supplementation on selected inflammatory biomarkers in older adults using secondary data from a randomised, placebo-controlled trial found little evidence of an effect of vitamin D supplementation on cytokine or adipokine levels, with the possible exception of IL-6 ( 35 ) . A recent study ( 36 ) , investigating the association between serum 25(OH)D and CRP and a potential causal effect by using genetic variants in bi-directional Mendelian randomisation analysis, in the Rotterdam Study, a prospective population-based cohort, showed that serum vitamin D was inversely associated with CRP.…”