Effect of Vipera ammodytes ammodytes Snake Venom on the Human Cytokine Network

Boda, Francisc; Banfai, Krisztina; Garai, Kitti; Curticăpean, Augustin; Berța, Lavinia; Sipos, Eszter; Kvell, Krisztián

doi:10.3390/toxins10070259

Cited by 9 publications

(6 citation statements)

References 56 publications

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“…Although the selected time points and concentrations might not overlap with lethal doses or therapeutic concentrations, these parameters were appointed for use in Petridish cultured cells in our experiments. The selected concentration also correspond to previously reported values [44,50,51].…”

Section: Effect Of Incubation Time and Venom Concentrationsupporting

confidence: 71%

“…Protective effects of BCL-2 under TRAIL-induced apoptosis was demonstrated by Sun et al [ 96 ]. Snake venoms have been reported to contribute to the activation of the cytokine network and induce intracellular inflammatory processes [ 44 ]. Similarly, in the present study, a tendency towards over-expression of TNFs was observed.…”

Section: Discussionmentioning

confidence: 99%

“…In the second experiment, thymic epithelial cells were treated with 0.1, 1.0 and 10 μg/mL BG or DA venom solutions, followed by incubation in identical conditions, but for 24 hours. The selection of the concentration range was based on previous experiences involving snake venoms and TaqMan Array technology, an experiment during which different concentrations of snake venoms solutions have been used on cell cultures, resulting in the observation that relatively high venom concentrations are required to induce a measurable effect in gene expression of ex vivo cell cultures [ 44 ]. Cells incubated without treatment served as negative control in both experiments.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Bitis gabonica and Dendroaspis angusticeps snake venoms on apoptosis-related genes in human thymic epithelial cells

Boda

Banfai

Garai

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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Section: Effect Of Incubation Time and Venom Concentrationsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Effect of Bitis gabonica and Dendroaspis angusticeps snake venoms on apoptosis-related genes in human thymic epithelial cells

Boda

Banfai

Garai

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

Self Cite

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“…After 6 h and 24 h of venom inoculation, the production of TNF-α, IFN-γ, IL-1β, IL-17, IL-4 and IL-10 were detected [25]. In another study, after treating U937 cells with Vipera ammodytes ammodytes venom, an increase in the expression of several inflammatory genes was observed, such as IL-1β and IL-8 [26]. The Vipera berus venom, another member of the Viperinae snake subfamily, caused edema, hemorrhage and myonecrosis in a model of inflammation induced in the cremaster muscle of mice [27].…”

Section: Introductionmentioning

confidence: 93%

Bitis arietans Snake Venom Induces an Inflammatory Response Which Is Partially Dependent on Lipid Mediators

Megale

Portaro

Silva

2020

Toxins

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Bitis arietans is a snake of medical importance, as it is responsible for more accidents in humans and domestic animals than all other African snakes put together. The accidents are characterized by local and systemic alterations, such as inflammation, cardiovascular and hemostatic disturbances, which can lead victims to death or permanent disability. However, little is known about the envenomation mechanism, especially regarding the inflammatory response, which is related to severe clinical conditions triggered by the venom. Therefore, the aim of the present study was to evaluate the inflammatory response related to the B. arietans envenomation using a peritonitis mice model. By pharmacological interventions and use of mice genetically deficient of the 5-lipoxygenase enzyme (5-LO−/−) or platelet-activating factor (PAF) receptor (PAFR−/− the participation of eicosanoids and PAF in this response was also investigated. The obtained results demonstrated that the venom induces an in vivo inflammatory response, characterized by an early increased vascular permeability, followed by an accumulation of polymorphonuclear (PMN) cells in the peritoneal cavity, accompanied by the production of the eicosanoids LTB4, LTC4, TXB2 and PGE2, as well as the local and systemic production of IL-6 and MCP-1. These inflammatory events were attenuated by the pre-treatment with anti-inflammatory drugs that interfere in lipid mediators’ functions. However, 5-LO−/− mice did not show a reduction of inflammatory response induced by the venom, while PAFR−/− mice showed a reduction in both the PMN leukocytes number and the local and systemic production of IL-6 and MCP-1. This study demonstrated that the Bitis arietans venom contains toxins that trigger an inflammatory process, which is partially dependent on lipid mediators, and may contribute to the envenomation pathology.

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“…Severe clinical manifestation includes local damage ( e . g ., painful oedema, tissue necrosis) and systemic injuries, including haemorrhage, coagulopathy and thrombocytopenia, critically resulting in high mortality and morbidity [ 5 , 9 ].With advanced proteomic technology, the heterogeneity of snake venoms has progressively been elucidated. For the Ovophis spp., venomic profiles of O .…”

Section: Introductionmentioning

confidence: 99%

Proteomics and immunocharacterization of Asian mountain pit viper (Ovophis monticola) venom

et al. 2021

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The venomic profile of Asian mountain pit viper Ovophis monticola is clarified in the present study. Using mass spectrometry-based proteomics, 247 different proteins were identified in crude venom of O. monticola found in Thailand. The most abundant proteins were snake venom metalloproteases (SVMP) (36.8%), snake venom serine proteases (SVSP) (31.1%), and phospholipases A2 (PLA2) (12.1%). Less abundant proteins included L-amino acid oxidase (LAAO) (5.7%), venom nerve growth factor (3.6%), nucleic acid degrading enzymes (3.2%), C-type lectins (CTL) (1.6%), cysteine-rich secretory proteins (CRISP) (1.2%) and disintegrin (1.2%). The immunoreactivity of this viper’s venom to a monovalent antivenom against green pit viper Trimeresurus albolabris, or to a polyvalent antivenom against hemotoxic venom was investigated by indirect ELISA and two-dimensional (2D) immunoblotting. Polyvalent antivenom showed substantially greater reactivity levels than monovalent antivenom. A titer for the monovalent antivenom was over 1:1.28x107 dilution while that of polyvalent antivenom was 1:5.12x107. Of a total of 89 spots comprising 173 proteins, 40 spots of predominantly SVMP, SVSP and PLA2 were specific antigens for antivenoms. The 49 unrecognized spots containing 72 proteins were characterized as non-reactive proteins, and included certain types of CTLs and CRISPs. These neglected venom constituents could limit the effectiveness of antivenom-based therapy currently available for victims of pit viper envenomation.

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Effect of Vipera ammodytes ammodytes Snake Venom on the Human Cytokine Network

Cited by 9 publications

References 56 publications

Effect of Bitis gabonica and Dendroaspis angusticeps snake venoms on apoptosis-related genes in human thymic epithelial cells

Effect of Bitis gabonica and Dendroaspis angusticeps snake venoms on apoptosis-related genes in human thymic epithelial cells

Bitis arietans Snake Venom Induces an Inflammatory Response Which Is Partially Dependent on Lipid Mediators

Proteomics and immunocharacterization of Asian mountain pit viper (Ovophis monticola) venom

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