Effect of Vichy water on catalase activity in the stratum corneum

Abstract

Synopsis Vichy spa water is essentially known for its therapeutic action on liver and bile duct functions. Its mechanism of action may be partially explained by the activation of certain digestive enzymes. A literature survey showed that Vichy water has also been used for local application in the treatment of certain dermatoses. Based on these data, the effects of Vichy spa water on the skin were studied using cutaneous enzymatic systems. The first studies were carried out on catalase, an oxidoreductase. The results showed a statistically significant increase (p<0.05) in the activity of the enzyme in the presence of Vichy spa water both in vitro and in vivo. Considering the involvement of catalase in skin defence against oxygen-derived free radicals generated, its increased activity may explain the beneficial role of Vichy water observed in various dermatoses.

Vichy mineralizing water with hyaluronic acid is effective and well tolerated as an adjunct to the management of various dermatoses and after esthetic procedures

“…3 Mineral 89 (M89, Laboratoires Vichy) contains 89% Vichy mineralizing water (VMW) as a hydrating agent and 0.4% hyaluronic acid (HA); an extracellular matrix component with viscoelastic and hygroscopic properties is able to control diverse cell processes and transepidermal water loss. 9 These primary antioxidant enzymes convert 2 potentially harmful reactive oxygen species, superoxide and hydrogen peroxide, to water. [4][5][6][7][8] In vitro and in vivo studies showed that VMW induced a statistically significant increase (P < .05) in the catalase enzyme activity.…”

“…[4][5][6][7][8] In vitro and in vivo studies showed that VMW induced a statistically significant increase (P < .05) in the catalase enzyme activity. 9 These primary antioxidant enzymes convert 2 potentially harmful reactive oxygen species, superoxide and hydrogen peroxide, to water. 5,10 It has also shown to have anti-inflammatory activity.…”

Vichy Thermal Spring Water (VTSW), a cosmetic ingredient of potential interest in the frame of skin ageing exposome: an in vitro study

“…Among these, prevention of radical species production (NQO1), activation of the thioredoxin system (TXNRD1) (one of the major antioxidant defense systems) linked to peroxyredoxines’ activation (PRDX1,2,3 and 6) , reduction (GPX2) and dismutation of H 2 O 2 (CAT) are potentially activated in keratinocyte response to VTSW. Of note, the activation of catalase (CAT) was previously measured in the stratum corneum, after in vivo application of VTSW to human skin . However, considering the weak induction of in vitro CAT gene expression (not confirmed by qRT/PCR, Table ), this in vivo CAT activation by VTSW was more likely linked to the VTSW high hydrogenocarbonate content and buffering effect around pH 7.0.…”

“…Among these, prevention of radical species production (NQO1), activation of the thioredoxin system (TXNRD1) (one of the major antioxidant defense systems) linked to peroxyredoxines' activation (PRDX1,2,3 and 6) [67], reduction (GPX2) and dismutation of H 2 O 2 (CAT) are potentially activated in keratinocyte response to VTSW. Of note, the activation of catalase (CAT) was previously measured in the stratum corneum, after in vivo application of VTSW to human skin [30].…”

“…Of note, Vichy Thermal Spring Water (VTSW) is characterized by a very specific ionic composition (Table ). It was previously found to stimulate the activity of Stratum corneum Catalase, an antioxidant enzyme that degrades hydrogen peroxide (H 2 O 2 ) into water and oxygen, H 2 O 2 being a potent initiator of ROS . To further characterize the effects of VTSW on skin cells, normal human keratinocytes were grown in vitro in the presence or absence of VTSW, and a full genome gene expression profile was established.…”