Effect of vegetable oils in the secretion of lipase from
            <i>Candida rugosa</i>
            (DSM 2031)

Lakshmi, Baddireddi Subhadra; Kangueane, Pandjassarame; Abraham, Bamisaye F.; Pennathur, Gautam

doi:10.1046/j.1365-2672.1999.00578.x

Cited by 52 publications

(39 citation statements)

References 14 publications

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“…According to Nunes et al [49] and Papanikolaou et al [50], among different vegetable oils, olive oil is considered as the best inducer of lipase production. The result, to some extent, also correlates with the findings by Lakshmi et al [51] that lipase production increases with relative increase of C18:n fatty acid esters in the respective vegetable oils, with olive oil having the highest percentage composition of C18:n fatty acid and coconut oil having the least [52]. Our results revealed olive oil as the highest lipase inducer and coconut oil as the lowest inducer of lipase production (Fig.…”

Section: Resultssupporting

confidence: 92%

“…6) and this was followed closely by lactose. Lactose was reported as best carbon source for lipase production by Rhizopus MR12 [56], glucose as best carbon source for Rhizopus JK-1 [53] and dextrose as best for Pseudomonas aeruginosa [51]. Among the various organic and inorganic nitrogen sources tested, maximum lipase production (29.43 U/ml) was observed with yeast extract (Fig.…”

Section: Resultsmentioning

confidence: 89%

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Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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This study investigated the screening, production and optimization of an extracellular lipase from a fungus isolated from the contaminated soil of a palm oil processing shed. This was with a view to obtaining a strain that can secrete lipase with biochemical properties exploitable for biotechnological applications such as bioremediation of oil contaminated sites. Soil samples were collected from palm oil contaminated sites in Gbogan, Osun State, Nigeria (Latitude N 7°29.1481´ and Longitude E 4°20.7587´). The isolated fungal strains were screened on tributyrin agar for exogenous lipolytic activity. Molecular identification was carried out by amplifications of ITS-1, 5.8S and ITS-2 regions. The effects of incubation time, inducers, pH, temperature, carbon and nitrogen sources were varied for optimal lipase production using one factor at a time approach. Rhizopus oryzae ZAC3 (NCBI accession No: KX035094) was identified as the highest lipaseproducing strain. Maximum lipase production was observed on the fourth day, pH 5.0 and a temperature of 45 o C. Olive oil, xylose and yeast extract were the best inducer, carbon and nitrogen sources respectively for lipase production. There was a 2.02 fold increase in lipase production under these optimized conditions. In conclusion, Rhizopus oryzae ZAC3 lipase has properties exploitable for industrial and biotechnological applications.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 89%

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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“…Similar outcome was encountered by Lakshmi et al (1999) where lipase production by Candida rugosa severely dropped with the combination of glucose in the medium. Combination of glucose and inducer eventually represses lipase production suggesting the occurrence of carbon catabolite repression.…”

Section: And 65supporting

confidence: 68%

Rhodococcus UKMP-5M, an endogenous lipase producing actinomycete from Peninsular Malaysia

2013

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Escalation in food industries unctuous wastes has led to serious anthropogenic problems to the environment. Parallel to "green strategy", growing awareness in biological treatment emphasizes efficacy of enzymatic technology for bioremediation. Pertinently, researchers are in search for new lipase-lipid interaction for improved outcome. Rhodococcus species have documented inadequate evidences on lipase enzyme production. Consequent assessments on Rhodococcus isolates from Peninsular Malaysia have identified twelve promising strains as lipase producer. Interestingly, apart from usual lipolytic behaviour, Rhodococcus sp. exhibited significant level of lipase endogenously, while cryogenic grinding method effectively ruptured the cell. An isolate from petroleum-contaminated site, namely Rhodococcus UKMP-5M, projected the highest level of lipase specificity and has further been optimized. It was found out that the best specificity was apparent in acidic condition (pH 5) with 6% inoculum at 30• C for 72 hours of incubation. Due to high level of mycolic cell-surfactant developed in triacylglycerol supplements, cell lysis was employed with Triton X-100 detergent solubilisation. As a result, oil blend composed of various carbon-chain length fatty acids (composite 2) induces enzyme production extensively. Remarkably, R. UKMP-5M found to cater enzyme production without aid of inducer by nature, but additional carbon source like glucose represses lipase production. Further ability for biological treatment was revealed when the optimized R. UKMP-5M whole cell degraded waste cooking oil significantly by solubilizing fatty acids and commencing conversion into biomass. These qualities resemble practical new lipid-lipase biological lipid rich on-site treatment.

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“…At low concentrations, oleic acid seems to favor the synthesis and secretion of Lip2 and Lip3 lipase isoenzymes while Lip1 is only produced at high oleic acid concentration [60]. Lipase production was also reported in the presence of carbon sources such as sterols [61], hexadecane [61], carboxylic acids [59], vegetable oils [62] and dodecanol [63]. By contrast, no lipase production could be detected in the presence of alkane, despite this carbon source could support cell growth [58].…”

Section: Regulation Of Lipase Production Concerning the Medium Composmentioning

confidence: 99%

Fungal Lipase Production by Solid-State Fermentation

Ramos-Sánchez¹,

Cujilema-Quitio²,

Julián-Ricardo³

et al. 2015

J Bioprocess Biotech

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Lipases are one of the most promising enzymes in the chemical and biopharmaceutical industries. Numerous applications have been reported including fine chemistry, detergents formulation and biodiesel synthesis. Lipases are commonly produced by a wide variety of yeasts and filamentous fungi in submerged fermentation or solid-state fermentation. Filamentous fungi and yeasts usually behave more efficiently in solid-state fermentation and show greater productivities when compared to submerged fermentation. Although filamentous fungi adequately growth in solid-state fermentation, there are some limitations for cultivating them by this process. Here, a review is made about lipase production in solid-state fermentation. This includes the analysis of solid-state fermentation as a promising technology, characterization of growth media and ambient factors such as moisture, pH and temperature.

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Effect of vegetable oils in the secretion of lipase from Candida rugosa (DSM 2031)

Cited by 52 publications

References 14 publications

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Rhodococcus UKMP-5M, an endogenous lipase producing actinomycete from Peninsular Malaysia

Fungal Lipase Production by Solid-State Fermentation

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