Effect of Various Stress Factors on the Induction of Androgenesis in Anther Cultures of Brussels Sprouts (<i>Brassica oleracea</i> L. var. <i>gemmifera</i>)

Krzyżanowska, D.; Górecka, Krystyna

doi:10.2478/v10032-008-0015-2

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…It is evident from present study that the androgenic response in Brassica increases with increase in incubation temperature. In brussels sprouts, maximum androgenesis has been observed under 35°C incubation temperature, in all the six varieties that has been used in experiment (Krzyzanowska and Gorecka 2008). The temperature stress treatment requirements are genotype specific and need to be optimized before routine application of haploid development program.…”

Section: Resultsmentioning

confidence: 99%

Effect of incubation temperature and sucrose concentration on callus induction and plant regeneration in cauliflower (Brassica oleracea var. botrytis)

Singh,

Devi,

Sarao

2021

VegSci

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Androgenic and plant regeneration potential from anther culture were investigated in cauliflower genotypes under different incubation temperatures, sucrose concentrations and regeneration media compositions. Anthers were cultured on MS medium supplemented with 1.5 mg/L 2,4-D and 1.0 mg/L NAA growth hormone with four different treatments of sucrose i.e. 3%, 5%, 10% and 13%. Maximum androgenesis has been observed with 10% sucrose concentration. Anthers of all the varieties were incubated under three different temperatures i.e. 25°C, 32°C and 35°C. Among these incubation temperatures, higher androgenesis was observed at 32°C incubation temperature. Callus were cultured on five shoot regeneration media compositions, among which maximum regeneration has been observed on MS media supplemented with 5.0 mg/L BAP and 1.0 mg/L NAA followed by MS media supplemented with 6.0 mg/L BAP and 1.0 mg/L NAA. Maximum root regeneration has been observed in ½ MS medium supplemented with 1.0 mg/ L IBA growth hormone followed by ½ MS medium supplemented with 0.4 mg/L IBA. In cytological examination, 20.3% of the regenerated plants were found to be haploid while 29.8% were spontaneous doubled haploids.

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Section: Resultsmentioning

confidence: 99%

Effect of incubation temperature and sucrose concentration on callus induction and plant regeneration in cauliflower (Brassica oleracea var. botrytis)

Singh,

Devi,

Sarao

2021

VegSci

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“…Dissection of anther was a kind of wounding or mechanical stress that stimulated androgenic embryo formation in some plants. For example, excision of anther tip was also found to enhance this process in white cabbage (Osolnik et al, 1993) and brussels sprout (Krzyżanowska & Górecka, 2008). The procedure used for androgenic embryo formation in this study was slightly adapted from a similar research of Sudhersan et al (2008) on Sturt's desert pea (an ornamental legume which is like butterfly pea belonging to the family Fabaceae).…”

Section: Resultsmentioning

confidence: 99%

Direct androgenesis in anther culture of butterfly pea related to flower development

Bodhipadma¹,

Noichinda²,

Onnui³

et al. 2017

j.appsci

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Androgenesis is a biotechnological pathway of in vitro gametic embryogenesis with the potential leading to haploid plant production from male reproductive organ. Butterfly pea (Clitoria ternatea L.) is a versatile leguminous plant with the floral shape resembling a butterfly at full bloom. Based on the varying lengths of flower as well as petals and sepals, 5 stages of flower development were delineated. When anthers from flowers of these various stages were cultured on basal Murashige and Skoog medium supplemented with 3 mg/l 2,4-D and 60 or 80 g/l sucrose, it was found that anther-derived embryos (11.67 and 3.3% in the presence of 6 and 8% sucrose, respectively) were obtained only from stage 2 flowers. Light microscopic observation also revealed that the microspores of anthers isolated from flowers at stage 2 was uninucleate. This suggested that anther or microspore stage of butterfly pea played a very important role in direct androgenesis and 8% sucrose in the medium was detrimental to this process.

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Enhanced regeneration of haploid plantlets from microspores of Brassica napus L. using bleomycin, PCIB, and phytohormones

Ahmadi

Alizadeh

Silva

2012

Plant Cell Tiss Organ Cult

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Effect of Various Stress Factors on the Induction of Androgenesis in Anther Cultures of Brussels Sprouts (Brassica oleracea L. var. gemmifera)

Cited by 4 publications

References 16 publications

Effect of incubation temperature and sucrose concentration on callus induction and plant regeneration in cauliflower (Brassica oleracea var. botrytis)

Effect of incubation temperature and sucrose concentration on callus induction and plant regeneration in cauliflower (Brassica oleracea var. botrytis)

Direct androgenesis in anther culture of butterfly pea related to flower development

Enhanced regeneration of haploid plantlets from microspores of Brassica napus L. using bleomycin, PCIB, and phytohormones

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