Effect of Various Parameters on the Growth and Ethanol Production by Yeasts Isolated from Natural Sources

Rahman, Shafkat Shamim; Hossain, Md. Altab; Choudhury, N.

doi:10.3329/bjm.v30i1-2.28453

Cited by 16 publications

(21 citation statements)

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“…After inoculation, MIU media (MIU test [ 25 ]) and Simmon’s agar slants were also incubated for 24-h at 37 °C [ 22 , 23 ]. Trypticase 10 g/L, NaCl 5 g/L, phenol red 0.018/L, sugar (glucose, lactose or sucrose) 5 g/L contained broth incubated at 37 °C for 24-h to determine the capability of fermenting carbohydrate substrate with acid and gas production [ 26 ]. Autoclaved starch agar (beef extract 3 g/L, soluble starch 10 g/L and agar 15 g/L) plates were incubated for 24-h to indicate the presence of α-amylase [ 27 ].…”

Section: Main Textmentioning

confidence: 99%

Isolation and identification of halotolerant soil bacteria from coastal Patenga area

2017

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ObjectiveHalotolerant bacteria have multiple uses viz. fermentation with lesser sterility control and industrial production of bioplastics. Moreover, it may increase the crop productivity of coastal saline lands in Bangladesh by transferring the salt tolerant genes into the plants. The study focused on the isolation and identification of the halotolerant bacteria from three soil samples, collected from coastal Patenga area. The samples were inoculated in nutrient media containing a wide range of salt concentrations.ResultsAll the samples showed 2, 4 and 6% (w/v) salt tolerance. The isolates from Patenga soil (4, 6%) and beach soil (2%) showed catalase activity and all the isolates showed negative results for oxidase activity, indole production, lactose and motility. All the samples provided positive results for dextrose fermentation. Other tests provided mixed results. Based on the morphological characteristics, biochemical tests and ABIS software analysis the isolates fall within the Enterobacteriaceae, Clostridium and Corynebacterium, with a predominance of Vibrios. Overall the isolates can be considered as mild halotolerant, with the best growth observed at lower salinities and no halophilism detected. Among many possibilities, the genes responsible for the salt tolerant trait in these species can be identified, extracted and inserted into the crop plants to form a transgenic plant to result in higher yield for the rest of the year.

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Section: Main Textmentioning

confidence: 99%

Isolation and identification of halotolerant soil bacteria from coastal Patenga area

2017

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“…The kitchen waste medium contained plant organelles and was a rich source of cellulose, starch and glucose monomers. Comparison with previous studies 15,16,18 showed the achieved production efficiency is below the level required for profitable commercial production. In this study, production of 7.3% (v/v) ethanol was recorded (Table 1).…”

Section: Discussionmentioning

confidence: 61%

“…Previous investigations 15,16,18 indicated that ethanol is produced more readily under shaking than non-shaking conditions. After 48 h of fermentation at room temperature in a rotary incubator (120 rpm), a maximum of 7.3% (v/v) ethanol production was recorded ( Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Yeast samples (Saccharomyces cerevisiae) were isolated and identified from date-juice using previously described methods 15,18 in YEPD medium (10 6 -10 7 cells/ml; 0.3% yeast extract #Y1625, 1% peptone #P7750, 2% dextrose #G8270, 1.5% agar #A1296, pH: 5; Sigma-Aldrich, St Louis, MO, USA). Discarded solid kitchen waste was collected from different households.…”

Section: Composition and Pretreatmentmentioning

confidence: 99%

“…Alongside a mainstream project 15,16 , in this study, tentative fermentation was carried out in kitchen waste medium to find out if a wild-type microorganism has the ability to ferment cellulose efficiently 17 .…”

Section: Introductionmentioning

confidence: 99%

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Bioethanol fermentation from kitchen waste using Saccharomyces cerevisiae

2018

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Bioethanol obtained from microbial fermentation can replace conventional fossil fuels to satisfy energy demand. In this respect, a fermenting isolate of Saccharomyces cerevisiae, obtained from date juice, was grown in YEPD medium as a part of a previous published research project. In this study, the isolate was tentatively characterized for alcoholic fermentation in organic kitchen waste medium, prepared from discarded fruit and vegetable peels. Fermentation in shaking condition resulted in the production of 7.3% (v/v) ethanol after 48 h, after which the pH of the medium increased slightly in response. Further research should be conducted to assess the potential of kitchen waste as a raw material in ethanol fermentation.

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Optimization of Bioethanol Production Using Response Surface Methodology for Stress‐Tolerant Wild Yeasts Isolated from Natural Forests

Tadesse,

Dase,

Koricha

et al. 2024

International Journal of Energy Research

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Yeasts are essential microorganisms for the ethanol production, and their efficiency depends on the physicochemical characteristics of the fermenting substrate. These microorganisms can be found in various soil and plant sources, such as leaves, bark, and litter of natural forests. The goal of the current study was to assess ethanol production using stress‐tolerant yeasts isolated from leaves, litters, rhizosphere soils, and tree barks collected from three natural forests (Belete‐Gera, Boter‐Becho, and Yayu Biosphere Reserve) in Southwest Ethiopia. Matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) was used to identify the isolates. Design‐Expert 11.1 (response surface methodology) was used to optimize the fermentation condition for effective ethanol production. A total of 406 yeasts were isolated, of which 67% were capable of ethanol production while 33% were not producers. The strongly fermentative isolates belonged to three genera: Saccharomyces cerevisiae, Meyerozyma guilliermondii, and Candida pelliculosa. S. cerevisiae 9Li2 and 35L1 were excellent candidates for ethanol production, with S. cerevisiae 9Li2 producing the highest ethanol (19.05 g/L) at optimal conditions (pH 5.09, 61.19 hours, and 30°C), while S. cerevisiae 35L1 produced maximum ethanol (19.03 g/L) at pH 4.92 and 30.03°C, within 71.55 hours. On the other hand, S. cerevisiae 9Li2 was able to ferment 40 g/L of glucose to produce 15 g/L of ethanol at 40°C with 73% efficiency at the ideal temperature (40°C), pH (5.167), and duration (72 hours), with a desirability of 0.819. The highest ethanol production (99.5 g/L) was achieved by S. cerevisiae 9Li2 after 48 hours at pH 5.0 and 30°C using 200 g/L of glucose. This work shows that multi‐stress‐tolerant S. cerevisiae isolates can thrive in harsh environments and should be enhanced for industrial bioethanol production exploiting their promising fermentation capacity.

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Effect of Various Parameters on the Growth and Ethanol Production by Yeasts Isolated from Natural Sources

Cited by 16 publications

References 4 publications

Isolation and identification of halotolerant soil bacteria from coastal Patenga area

Isolation and identification of halotolerant soil bacteria from coastal Patenga area

Bioethanol fermentation from kitchen waste using Saccharomyces cerevisiae

Optimization of Bioethanol Production Using Response Surface Methodology for Stress‐Tolerant Wild Yeasts Isolated from Natural Forests

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