Effect of Various Oxygen and Carbon Dioxide Concentrations on Cotton Root Development

Leonard, O. A.; Pinckard, J. A.

doi:10.1104/pp.21.1.18

Cited by 49 publications

(26 citation statements)

References 7 publications

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“…This was true for all ethylene concentrations tested. Stolwijk and Thimann (18) and others (6,8,14,19) (8,19). Just as in the case of root elongation, our results suggest that the antagonism of applied CO2 and endogenous ethlylene could be responsible for these observations.…”

Section: Resultssupporting

confidence: 52%

“…Many re'ports have indicated that the presence of CO2 at such levels is stimulatory to root growth (6,8,14,18,19) but the mechanism of this response is not known. Such an alteration of root growth patterns by CO2 might result from an interaction with ethylene; and one can thus propose that, in such a sys,tem, CO2 acts to prevent inhibition of growth by ethylene.…”

mentioning

confidence: 99%

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Ethylene and Carbon Dioxide in the Growth and Development of Cultured Radish Roots

Radin

Loomis

1969

Plant Physiol.

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Abstract. Ethylene is produced by cultured radish roots in amounts large enough to be physiologically important. When roots were grown in oontrolled atmospheres, applied ethylene was generally inhibitory to elongation, lateral root initiation, and cambial activity. 1 % CO2 similarly affected roots not given ethylene. In contrast, elongation and lateral root production of ethylene-treated roots were stimulated by 1 % COO. The results suggest that the oftenobserved stimulation of root growth by CO2 is due to an interaction with endogenous ethylene.

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Section: Resultssupporting

confidence: 52%

mentioning

confidence: 99%

Ethylene and Carbon Dioxide in the Growth and Development of Cultured Radish Roots

Radin

Loomis

1969

Plant Physiol.

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“…The incorporation of radioactive leucine into soluble protein was linear for up to 3 hours (Fig. 1); thus, different levels of radioactivity after a 90-min incubation (Tables I to IV) (1,5,7,11,17). Splittstoesser (16) proposed that CO2-stimulated growth involved dark CO2 fixation and the consequent synthesis of 4-carbon acids which can replace Krebs acid lost to the Krebs cycle during the biosynthesis of amino acids and other metabolites.…”

Section: Methodsmentioning

confidence: 99%

The Influence of 0.03% Carbon Dioxide on Protein Metabolism of Etiolated Avena sativa Coleoptiles

Bown¹,

Aung²

1974

Plant Physiol.

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The influence of indoleacetic acid, 0.03 % C02, and malate on protein metabolism of etiolated Avena sativa coleoptile sections has been investigated. All three were found to elevate both the rate of incorporation of labeled leucine into protein, and the level of soluble protein. The synergistic relationship between IAA and 0.03% CO2 in stimulating the growth of etiolated Avena coleoptile sections has been previously described (1), and the involvement of protein synthesis in auxin-stimulated growth is well documented (4,10,13). Experiments reported here were designed to study the separate and combined influence of IAA and CO2 on coleoptile protein metabolism, and to test the proposal that CO2-stimulated growth is mediated by a process involving both dark CO2 fixation and CO2-stimulated protein synthesis (16). The results suggest that both IAA and CO2 stimulate protein synthesis, that CO2-stimulated protein synthesis involves dark CO2 fixation, and that elevated rates of protein synthesis result in increased incorporation of labeled bicarbonate into protein. It is proposed that CO2-stimulated growth is dependent on C02-stimulated protein synthesis. MATERIALS AND METHODS"4C-sodium bicarbonate (59 mc/mmole) and L-"C(U)leucine (331 mc/mmole) were purchased from Amersham Searle; cycloheximide and IAA were purchased from the Sigma Chemical Company. Seeds of Avena sativa var. 'Victory' were grown and harvested as described previously (1). Except where mentioned, the 20-mm coleoptile section below the 3-mm tip was used. Incubation Conditions. Weighed batches of tissue in 50-ml light-tight incubation tubes were submerged in 10 ml of phosphate buffer (25 mm, pH 7.5) and incubated at 25 C in the presence or absence of various test substances. In experiments involving 14C-leucine, the buffer was aerated with air or CO2-free air at a rate of 10 I/hr batch of tissue; incubations involving "4C-bicarbonate were in closed tubes without aeration. The CO2 concentration used had no measurable influence on the pH of the incubation medium, and was measured with an infrared gas analyzer.Protein Determination. After incubation, tissue was washed thoroughly with water, frozen for 3 to 4 hr, thawed, and then ground vigorously in a Potter-Elvehjem tissue homogenizer for 5 min with 5 ml of phosphate buffer (1 mM, pH 7.5). The homogenate was centrifuged at 13,000g for 15 min, the supernatant fluid was collected, and the extraction procedure was repeated two more times with pH 7.5 phosphate buffer at 25 mm and 0.10 M. The three supernatant fluids were then combined. An equal volume of saturated ammonium sulfate was added to the combined extracts and, after 12 hr at 4 C, the precipitate was collected by centrifugation at 25,000g for 30 min. The pellet was washed with 5 ml of saturated ammonium sulfate containing nonradioactive leucine (0.1% w/v), centrifuged at 25,000g for 30 min, and then dissolved in phosphate buffer (25 mm, pH 7.5). Aliquots of this solution were used for soluble protein determination by the standard method of Lowry...

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“…However, various environmental factors such as light, temperature, oxygen, etc. play an important role for final yield as they affects the seed germination to emergence during the first 10 days [23]. Biofield treatment, could be a new and alternative approach to improve the overall growth, and yield of cotton crops.…”

Section: Effect Of Biofield On the Growth Of Young Cotton Seedlingsmentioning

confidence: 99%

Analysis of Genetic Diversity Using Simple Sequence Repeat (SSR) Markers and Growth Regulator Response in Biofield Treated Cotton (<i>Gossypium hirsutum</i> L.)

Trivedi¹

2015

AJAF

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Abstract:Cotton is the most important crop for the production of fiber that plays a key role in economic and social affairs. The aim of the study was to evaluate the impact of biofield energy treatment on cotton seeds regarding its growth, germination of seedling, glutathione (GSH) concentration, indole acetic acid (IAA) content and DNA fingerprinting using simple sequence repeat (SSR) markers for polymorphism analysis. The seeds of cotton cv. Stoneville-2 (Gossypium hirsutum L.) was obtained from DNA Land Marks Inc., Canada and divided into two groups. One group was remained as untreated, while the other was subjected to Mr. Trivedi biofield energy and referred as treated sample. The growth-germination of cotton seedling data showed higher germination (82%) in biofield treated seeds as compared to the control (68%). The alterations in length of shoot and root of cotton seedling was reported in the treated sample with respect to untreated seeds. However, the endogenous level of GSH in the leaves of treated cotton was increased by 27.68% as compared to the untreated sample, which may suggest an improved immunity of cotton plant. Further, the plant growth regulatory constituent i.e. IAA concentration was increased by 7.39%, as compared with the control. Besides, the DNA fingerprinting data, showed polymorphism (4%) between treated and untreated samples of cotton. The overall results suggest that the biofield energy treatment on cotton seeds, results in improved overall growth of plant, increase germination rate, GSH and IAA concentration were increased. The study assumed that biofield energy treatment on cotton seeds would be more useful for the production of cotton fiber.

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Effect of Various Oxygen and Carbon Dioxide Concentrations on Cotton Root Development

Cited by 49 publications

References 7 publications

Ethylene and Carbon Dioxide in the Growth and Development of Cultured Radish Roots

Ethylene and Carbon Dioxide in the Growth and Development of Cultured Radish Roots

The Influence of 0.03% Carbon Dioxide on Protein Metabolism of Etiolated Avena sativa Coleoptiles

Analysis of Genetic Diversity Using Simple Sequence Repeat (SSR) Markers and Growth Regulator Response in Biofield Treated Cotton (<i>Gossypium hirsutum</i> L.)

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Effect of Various Oxygen and Carbon Dioxide Concentrations on Cotton Root Development

Cited by 49 publications

References 7 publications

Ethylene and Carbon Dioxide in the Growth and Development of Cultured Radish Roots

Ethylene and Carbon Dioxide in the Growth and Development of Cultured Radish Roots

The Influence of 0.03% Carbon Dioxide on Protein Metabolism of Etiolated Avena sativa Coleoptiles

Analysis of Genetic Diversity Using Simple Sequence Repeat (SSR) Markers and Growth Regulator Response in Biofield Treated Cotton (&lt;i&gt;Gossypium hirsutum&lt;/i&gt; L.)

Contact Info

Product

Resources

About

Analysis of Genetic Diversity Using Simple Sequence Repeat (SSR) Markers and Growth Regulator Response in Biofield Treated Cotton (<i>Gossypium hirsutum</i> L.)