Analysis of Genetic Diversity Using Simple Sequence Repeat (SSR) Markers and Growth Regulator Response in Biofield Treated Cotton (<i>Gossypium hirsutum</i> L.)

Abstract

Abstract:Cotton is the most important crop for the production of fiber that plays a key role in economic and social affairs. The aim of the study was to evaluate the impact of biofield energy treatment on cotton seeds regarding its growth, germination of seedling, glutathione (GSH) concentration, indole acetic acid (IAA) content and DNA fingerprinting using simple sequence repeat (SSR) markers for polymorphism analysis. The seeds of cotton cv. Stoneville-2 (Gossypium hirsutum L.) was obtained from DNA Land Marks Inc., Canada and divided into two groups. One group was remained as untreated, while the other was subjected to Mr. Trivedi biofield energy and referred as treated sample. The growth-germination of cotton seedling data showed higher germination (82%) in biofield treated seeds as compared to the control (68%). The alterations in length of shoot and root of cotton seedling was reported in the treated sample with respect to untreated seeds. However, the endogenous level of GSH in the leaves of treated cotton was increased by 27.68% as compared to the untreated sample, which may suggest an improved immunity of cotton plant. Further, the plant growth regulatory constituent i.e. IAA concentration was increased by 7.39%, as compared with the control. Besides, the DNA fingerprinting data, showed polymorphism (4%) between treated and untreated samples of cotton. The overall results suggest that the biofield energy treatment on cotton seeds, results in improved overall growth of plant, increase germination rate, GSH and IAA concentration were increased. The study assumed that biofield energy treatment on cotton seeds would be more useful for the production of cotton fiber.

Study of the Energy of Consciousness Healing Treatment on Physical, Structural, Thermal, and Behavioral Properties of Zinc Chloride

“…The National Center of Complementary and Integrative Health (NCCIH) has been recognized and accepted Biofield Energy Healing as a Complementary and Alternative Medicine (CAM) health care approach in addition to other therapies, medicines and practices such as natural products, deep breathing, yoga, Tai Chi, Qi Gong, chiropractic/osteopathic manipulation, meditation, massage, special diets, homeopathy, progressive relaxation, guided imagery, acupressure, acupuncture, relaxation techniques, hypnotherapy, healing touch, movement therapy, pilates, rolfing structural integration, mindfulness, Ayurvedic medicine, traditional Chinese herbs and medicines, naturopathy, essential oils, aromatherapy, Reiki, cranial sacral therapy and applied prayer (as is common in all religions, like Christianity, Hinduism, Buddhism and Judaism) [20]. Biofield Energy Treatment (The Trivedi Effect ® ) has been reported in numerous peer-reviewed science journals with significant outcomes in many scientific fields such as cancer research [21]; altered antimicrobial sensitivity of pathogenic microbes in microbiology [22][23][24], biotechnology [25,26], genetics [27,28]; changing the structure of the atom in relation to various metals, ceramics, polymers and chemicals in materials science [29,30], altered physical and chemical properties of pharmaceuticals [31,32], nutraceuticals [33,34], organic compounds [35][36][37], and improved overall growth and yield of plants in agricultural science [38,39]. The physicochemical properties such as particle size, crystalline structure, crystallite size, surface area, and thermal properties of a drug have a vital role in bioavailability as well as stability of the drug during processing, formulation, storage, and packaging [40,41].…”

A Comprehensive Analytical Evaluation of the Trivedi Effect<sup>®</sup> - Energy of Consciousness Healing Treatment on the Physical, Structural, and Thermal Properties of Zinc Chloride

“…The National Center of Complementary and Integrative Health (NCCIH) has been recognized and accepted Biofield Energy Healing as a complementary and alternative medicine (CAM) health care approach in addition to other therapies, medicines and practices such as natural products, deep breathing, yoga, Tai Chi, Qi Gong, chiropractic/osteopathic manipulation, meditation, massage, special diets, homeopathy, progressive relaxation, guided imagery, acupressure, acupuncture, relaxation techniques, hypnotherapy, healing touch, movement therapy, pilates, rolfing structural integration, mindfulness, Ayurvedic medicine, traditional Chinese herbs and medicines, naturopathy, essential oils, aromatherapy, Reiki, cranial sacral therapy and applied prayer (as is common in all religions, like Christianity, Hinduism, Buddhism and Judaism) [20]. Biofield Energy Treatment (The Trivedi Effect ® ) has been reported in numerous peer-reviewed science journals with significant outcomes in many scientific fields such as cancer research [21]; altered antimicrobial sensitivity of pathogenic microbes in microbiology [22][23][24], biotechnology [25,26], genetics [27,28]; changing the structure of the atom in relation to various metals, ceramics, polymers and chemicals in materials science [29,30], altered physical and chemical properties of pharmaceuticals [31,32], nutraceuticals [33,34], organic compounds [35][36][37], and improved overall growth and yield of plants in agricultural science [38,39]. The physicochemical properties such as particle size, crystalline structure, crystallite size, surface area, etc.…”

Evaluation of the Physicochemical, Structural, Thermal, and Behavioral Properties of the Energy of Consciousness Healing Treated Zinc Chloride

“…The National Center of Complementary and Integrative Health (NCCIH) has been recognized and accepted Biofield Energy Healing as a Complementary and Alternative Medicine (CAM) health care approach in addition to other therapies, medicines and practices such as natural products, deep breathing, yoga, Tai Chi, Qi Gong, chiropractic/osteopathic manipulation, meditation, massage, special diets, homeopathy, progressive relaxation, guided imagery, acupressure, acupuncture, relaxation techniques, hypnotherapy, healing touch, movement therapy, pilates, rolfing structural integration, mindfulness, Ayurvedic medicine, traditional Chinese herbs and medicines, naturopathy, essential oils, aromatherapy, Reiki, cranial sacral therapy and applied prayer (as is common in all religions, like Christianity, Hinduism, Buddhism and Judaism) [20]. Biofield Energy Treatment (The Trivedi Effect ® ) has been reported in numerous peer-reviewed science journals with significant outcomes in many scientific fields such as cancer research [21]; altered antimicrobial sensitivity of pathogenic microbes in microbiology [22][23][24], biotechnology [25,26], genetics [27,28]; changing the structure of the atom in relation to various metals, ceramics, polymers and chemicals [29,30], altered physical and chemical properties of pharmaceuticals [31,32], nutraceuticals [33,34], organic compounds [35][36][37], and improved overall growth and yield of plants in agricultural science [38,39]. The physicochemical properties such as particle size, crystalline structure, crystallite size, surface area, and thermal properties of a drug have a vital role in bioavailability as well as stability of the drug during processing, formulation, storage, and packaging [40,41].…”