Effect of Various Concentrations of Brilliant Green and Bile Salts on Salmonellae and Other Microorganisms1

Abstract

MILLER, V. RICHARD (Purdue University, Lafayette, Ind.), AND GEORGE J. BAN-WART. Effect of various concentrations of brilliant green and bile salts on salmonellae and other microorganisms. Appl. Microbiol. 13:77-80. 1965.-A study of the inhibitory effect of 24 different combinations of brilliant green and bile salts concentrations was conducted, using seven species of microorganisms capable of fermenting mannitol. The inhibitory effect of brilliant green decreased as the bile salts concentration was increased. Staphylococcus aureus and Proteus rettgeri were inhibited by all test media. Escherichia coli was inhibited in all but two combinations of brilliant green and bile salts. Aerobacter aerogenes generally followed a pattern of growth similar to that of three species of salmonellae. Three of the 24 combinations of brilliant green and bile salts showed little or no inhibition of salmonellae but did inhibit the other organisms studied.

Evaluation of Culture Media for the Isolation of Salmonellae from Feces

“…Slavin (22) used Brilliant Green in the selective media in 100 ,ug/ml and 50 ,g/ml concentrations as he found that its inhibitory effect varied with the source of the dye. Miller and Banwart (20) indicated that Brilliant Green proportionately lost its inhibitive effect on bacteria in the presence of increasing amounts of organic matter. Smith (23) mentioned that the reason for failure to recover S. choleraesuis from feces was not primarily that the feces contained bacteria which outgrew salmonellae but that many of the media used were too toxic to permit the growth of the organism.…”

The isolation of salmonellas and campylobacters

“…typhi (Harvey 1956). The toxicity of brilliant green is substantially reduced by the presence of bile salts (Miller & Banwart 1965;Moats & Kinner 1974) and probably the most widely used brilliant green-containing plating medium is the brilliant green agar (BGA) described by van Leusden et al (1982). There have been many attempts to improve the selectivity of brilliant green agars but these have met with varying degrees of success.…”

Quality Control of Solid Culture Media: A Comparison of the Classic and the So‐called Ecometric Technique

“…These failures are often due to the formation of reaction products of hexoses with intrinsic antimicrobial properties (Davis & Rogers 1939;Tarkow et al 1942;Corper & Clark 1946;Foster 1952;Wilson & Brown 1953;Ingram et al 1955;McKeen 1956;Finkelstein & Lankford 1957;Jemmali 1969;Rosen et al 1970;Graumlich & Stevenson 1978). Many more observations demonstrate deficiencies of selective media, which rely on delicate equilibria between productive and selective mechanisms of various sorts (Kligler 1918;Banwart & Ayres 1953;Burman 1955;Miller & Banwart 1965;Read & Reyes 1968;Taylor & Schelhart 1968;Wilson et al 1970;Reusze 1971;McCormack et al 1974;Moats & Kinner 1974;Mossel et at. 1974;Harvey et al 1975;Fagerberg et al 1976;Nicholls et al 1976;Papadakis et al 1976;Price 1976;Staat 1976;Hanna et al 1977 should, therefore, be regular quality monitoring of all types of culture media (Mossel 1971;Vera 1971;Prier et al 1975;Stokes 1979).…”