Abstract:Liquid perfluorocarbon (PFC) instillation has been studied experimentally as an adjuvant therapy in the preservation of lung grafts during cold ischemia. The objective of this study was to evaluate whether vaporized PFC is also protective of lung grafts at different cold ischemia times. We performed histological analysis of and measured oxidative stress in the lungs of animals that received only preservation solution with low-potassium dextran (LPD) or vaporized PFC together with LPD. We conclude that vaporize… Show more
“…[11][12][13] For example, when an emulsified form of perfluorochemicals was administered directly into the blood vessels or the airway, relatively modest side effects were reported, including the elevation of blood pressure and injury to organs. 12,[17][18][19] The level of arterial oxygenation, if scaled for human application, is likely sufficient to alleviate patients with severe respiratory failure. -PFD potentially provides lifesaving oxygenation, while there is additional room for improving gas exchange efficacy.…”
Okabe et al. report the exploitation of intestinal breathing phenomenon in mammalian pre-clinical models, potentially offering an additional route of O 2 administration to patients who are in critical need of immediate respiratory support.
“…[11][12][13] For example, when an emulsified form of perfluorochemicals was administered directly into the blood vessels or the airway, relatively modest side effects were reported, including the elevation of blood pressure and injury to organs. 12,[17][18][19] The level of arterial oxygenation, if scaled for human application, is likely sufficient to alleviate patients with severe respiratory failure. -PFD potentially provides lifesaving oxygenation, while there is additional room for improving gas exchange efficacy.…”
Okabe et al. report the exploitation of intestinal breathing phenomenon in mammalian pre-clinical models, potentially offering an additional route of O 2 administration to patients who are in critical need of immediate respiratory support.
“…At the same time, PFC is easily distributed throughout the lungs in a more uniform manner during the vaporization process and will not cause lung ventilation difficulties caused by infusion of liquid PFC. In a cold ischemia rat model, vaporized PFC + LPD preserved lungs exhibited higher superoxide dismutase (SOD) concentrations than LPD alone, suggesting that use of vaporized PFC reduces free radical production, which in turn protects lung grafts from oxidative stress damage …”
Section: Application Of Oxygen Carriersmentioning
confidence: 99%
“…In a cold ischemia rat model, vaporized PFC + LPD preserved lungs exhibited higher superoxide dismutase (SOD) concentrations than LPD alone, suggesting that use of vaporized PFC reduces free radical production, which in turn protects lung grafts from oxidative stress damage. 103 3.2.3. Application of PFC Oxygen Carriers in Tumor Therapy.…”
Section: Application Of Pfc Oxygen Carriers In Organmentioning
Ischemia or hypoxia can lead to pathological changes in the metabolism and function of tissues and then lead to various diseases. Timely and effective blood resuscitation or improvement of hypoxia is very important for the treatment of diseases. However, there is a need to develop stable, nontoxic, and immunologically inert oxygen carriers due to limitations such as blood shortages, different blood types, and the risk of transmitting infections. With the development of various technologies, oxygen carriers based on hemoglobin and perfluorocarbon have been widely studied in recent years. This paper reviews the development and application of hemoglobin and perfluorocarbon oxygen carriers. The design of oxygen carriers was analyzed, and their application as blood substitutes or oxygen carriers in various hypoxic diseases was discussed. Finally, the characteristics and future research of ideal oxygen carriers were prospected to provide reference for follow-up research.
“…In previous studies, PFT was mentioned as potential preventive strategy for ischemia/reperfusion injuries for example in lung graft preservation (14). Also, it is suggested that PFT could decrease production of inflammatory markers and stress oxidative markers, decrease lung oedema and hydroxyproline level, as well as attenuate histopathological changes and fibrosis (15).…”
The aim of this research was to examine the influence of the intraperitoneal application of PFT in different doses and regimen on systemic oxidative stress and activity of antioxidative enzymes in animals. Depending on whether the animals received only saline or PFT in different doses (8, 12, 16 ml/kg body weight), and time (1, 10, or 20 hours before sacrificing and blood sampling), all animals were divided into control or experimental groups. From plasma samples we measured following biomarkers of oxidative stress: superoxide anion radical (O2
−), hydrogen peroxide (H2O2), nitrites (NO2
−), index of lipid peroxidation measured as TBARS (thiobarbituric acid reactive substances), and from hemolysate samples activity of the next enzymes: catalase (CAT), superoxidedismutase (SOD) and reduced glutathione (GSH). All mentioned biochemical parameters of oxidative stress were determined spectrophotometrically (Shimadzu UV-1800UV-VIS spectrophotometer, Japan). Superoxide anion radical was a molecule very affected with the PFT administration. we observed the significantly higher activity of superoxide dismutase in all PFT treated groups in comparison with the CTRL group. The highest activity was observed in group treated with the 8 and 12 ml/kg of PFT nearly to sampling (1 hour). Catalase activity was significantly higher in PFT group in comparison with the CTRL, especially in PFT 16ml/kg group (1 hour). In comparison with the CTRL group, the total content of GSH was significantly lower in the groups treated PFT in dose of 16 ml/kg 1 hour and 10 hours before blood sampling. All these changes in oxidative stress markers seems to be very clear, but we can observe that almost all changes are induced in 1 hour after PFT administration. Probably, PFT solution has short-term protective effects on reducing oxidative stress, but no long term-effects. Maybe the chemical and biological instability of PFT solution could be a reason for that transient antioxidative effects, and developing the nano-formulation of PFT could be potential option for resolving the problem with poor pharmacodynamic of PFT.
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