Effect of vanillin on methylene blue plus light-induced single-strand breaks in plasmid pBR322 DNA

Kumar, Sanjeev; Ghosh, Arna; Devasagayam, T.P.A.; Chauhan, Prashant

doi:10.1016/s1383-5718(00)00074-7

Cited by 33 publications

(17 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…VAN effectively inhibited lipid peroxidation [35][36][37], scavenged free radicals [38,39], and reduced the number of DNA single-strand breaks induced by singlet oxygen [40]. In addition to these antioxidant effects, VAN has been shown to inhibit mutagenicity induced by a variety of chemical and physical agents [1][2][3]8,9].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of spontaneous mutagenesis by vanillin and cinnamaldehyde in Escherichia coli: Dependence on recombinational repair

Shaughnessy

Schaaper

Umbach

et al. 2006

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

View full text Add to dashboard Cite

Vanillin (VAN) and cinnamaldehyde (CIN) are dietary antimutagens that effectively inhibit both induced and spontaneous mutations. We have shown previously that VAN and CIN reduced the spontaneous mutant frequency in Salmonella TA104 (hisG428, rfa, ΔuvrB, pKM101) by approximately 50% and that both compounds significantly reduced mutations at GC sites but not at AT sites. Previous studies have suggested that VAN and CIN may reduce mutations in bacterial model systems by modulating DNA repair pathways, particularly by enhancing recombinational repair. To further explore the basis for inhibition of spontaneous mutation by VAN and CIN, we have determined the effects of these compounds on survival and mutant frequency in five Escherichia coli strains derived from the wild-type strain NR9102 with different DNA repair backgrounds. At nontoxic doses, both VAN and CIN significantly reduced mutant frequency in the wild-type strain NR9102, in the nucleotide excision repair-deficient strain NR11634 (uvrB), and in the recombination-proficient but SOS-deficient strain NR11475 (recA430). In contrast, in the recombination-deficient and SOS-deficient strain NR11317 (recA56), both VAN and CIN not only failed to inhibit the spontaneous mutant frequency but actually increased the mutant frequency. In the mismatch repair-defective strain NR9319 (mutL), only CIN was antimutagenic. Our results show that the antimutagenicity of VAN and CIN against spontaneous mutation required the RecA recombination function but was independent of the SOS and nucleotide excision repair pathways. Thus, we propose the counterintuitive notion that these antimutagens actually produce a type of DNA damage that elicits recombinational repair (but not mismatch, SOS, or nucleotide excision repair), which then repairs not only the damage induced by VAN and CIN but also other DNA damageresulting in an antimutagenic effect on spontaneous mutation.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibition of spontaneous mutagenesis by vanillin and cinnamaldehyde in Escherichia coli: Dependence on recombinational repair

Shaughnessy

Schaaper

Umbach

et al. 2006

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

View full text Add to dashboard Cite

show abstract

“…Vanillin was found to provide effective DNA protection against photosensitization-induced ssbs (mainly type II reaction) in the absence of DNA repair, replication machinery, or other cellular defenses, which can be in part due to its ability to scavenge O 2 [23, 52]. On the other hand, vanillin was able to inhibit mutations at the CD59 locus in human-hamster hybrid A L cells induced by H 2 O 2 , MNNG, and MMC, but not 137 Cs γ -radiation.…”

Section: Antimutagenic Activitymentioning

confidence: 99%

Overview of the Role of Vanillin on Redox Status and Cancer Development

Bezerra

Soares

Sousa

2016

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Bioactive natural products play critical roles in modern drug development, especially anticancer agents. It has been widely reported that various pharmacological activities of such compounds are related to their antioxidant properties. Vanillin is a natural substance widely found in many plant species and often used in beverages, foods, cosmetics, and pharmaceutical products. Antioxidant and anticancer potential have been described for this compound. Considering the importance of vanillin in the area of human health and food and pharmaceuticals sectors, in this review, we discuss the role of vanillin on redox status and its potential contribution to the prevention and the treatment of cancer.

show abstract

“…14 Our earlier studies showed that this compound inhibited formation of DNA single strand breaks induced by singlet oxygen in the plasmid pBR322. 15 Recently, vanillin has Vanillin, a plant derived natural product, used as food flavoring agent and its positional isomer ovanillin, have been tested for their ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical using high performance liquid chromatography (HPLC). Trolox, a water-soluble analogue of vitamin E and a well-known antioxidant was used as a reference compound.…”

Section: Introductionmentioning

confidence: 99%

Free radical scavenging activity of vanillin ando-vanillin using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical

2002

View full text Add to dashboard Cite

Effect of vanillin on methylene blue plus light-induced single-strand breaks in plasmid pBR322 DNA

Cited by 33 publications

References 31 publications

Inhibition of spontaneous mutagenesis by vanillin and cinnamaldehyde in Escherichia coli: Dependence on recombinational repair

Inhibition of spontaneous mutagenesis by vanillin and cinnamaldehyde in Escherichia coli: Dependence on recombinational repair

Overview of the Role of Vanillin on Redox Status and Cancer Development

Free radical scavenging activity of vanillin ando-vanillin using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical

Contact Info

Product

Resources

About