Effect of Use of Platelet-Rich Plasma (PRP) in Skin with Intrinsic Aging Process

Charles-de-Sá, Luiz; Gontijo-de-Amorim, Natale Ferreira; Takiya, Christina Maeda; Borojević, Radovan; Benati, Donatella; Bernardi, Paolo; Sbarbati, Andrea; Rigotti, Gino

doi:10.1093/asj/sjx137

Cited by 38 publications

(32 citation statements)

References 19 publications

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“…A positive correlation between PRP and skin anti-aging has also been reported in both pre-clinical and clinical practice (25)(26)(27)(28). Consistent with previous studies (17,26), the present clinical study showed that PRP treatment improved skin conditions, including increased skin thickness, enhanced collagen content and reduced pigmentation. In addition, parameters assessed using the VISIA system, such as wrinkles, texture and pores were all decreased compared with pretreatment.…”

Section: Discussionsupporting

confidence: 91%

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Effects of autologous platelet‑rich plasma injections on facial skin rejuvenation

Lei

2020

Exp Ther Med

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Autologous serum platelet-rich plasma (PRP) has been used to rejuvenate wrinkled and aged skin for years; however, the molecular mechanism for the positive effects of PRP on the skin remains unclear. The present study aimed to clarify the potential molecular mechanisms for the role of PRP in wrinkled and aged skin rejuvenation, and provide evidence for future clinical applications. A total of 30 healthy females were recruited for PRP treatment and signed informed consent was obtained. A total of 3 autologous PRP injections were administered to each patient with 15-day intervals between injections. The effects of PRP injections were evaluated using the VISIA ® Complexion Analysis System and skin computed tomography. A human organotypic skin model was established and treated with PBS or PRP before ultraviolet (UV)-B light (10 mJ/cm 2) irradiation. The distribution of the epidermal structure and dermal fibers were analyzed by hematoxylin and eosin and Masson's trichome staining. Expression of matrix metalloproteinase-1 (MMP-1), tyrosinase, fibrillin and tropoelastin was detected by reverse transcription-quantitative PCR, western blotting and immunofluorescence. The present results showed that PRP treatment improved skin quality in the participants. In addition, the VISIA ® results showed that wrinkles, texture and pores were decreased in the PRP groups compared with the PBS treatment. The in vitro study demonstrated that PRP treatment ameliorated photoaging by inhibiting UV-B-induced MMP-1 and tyrosinase upregulation, and by inducing fibrillin and tropoelastin expression that was downregulated by UV-B. Collectively, it was demonstrated that PRP treatment ameliorated skin photoaging through regulation of MMP-1, tyrosinase, fibrillin and tropoelastin expression.

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Section: Discussionsupporting

confidence: 91%

“…These proteins can also act as a scaffold for nascent cells and tissues to promote the repair of damaged/aging skin. At the molecular level, PRP injection induces DNA synthesis and promotes the corresponding gene expression (16,17).…”

Section: Introductionmentioning

confidence: 99%

Effects of autologous platelet‑rich plasma injections on facial skin rejuvenation

Lei

2020

Exp Ther Med

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“…Considering our results with isolated use of PRP in the skin, we observed that treatment with PRP promoted unwanted changes in the reticular dermis, mainly due to the deposition of a horizontal layer of collagen (fibrosis) and elastic fibers tightly linked creating a type of barrier between sweat and sebaceous glands with subcutaneous adipose tissue at the dermoepidermal junction [ 67 ]. Structural analyses after treatment with PRP showed infiltration of the perivascular area with mononuclear cells, visible in the reticular dermis and dermoepidermal junction.…”

Section: Discussionmentioning

confidence: 99%

Photoaging Skin Therapy with PRP and ADSC: A Comparative Study

Charles-de-Sá

Gontijo-de-Amorim

Sbarbati

et al. 2020

Stem Cells International

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Background. Stem cells from adipose tissue (ADSCs) and platelet-rich plasma (PRP) are innovative modalities that arise due to their regenerative potential. Objective. The aim of this study was to characterize possible histological changes induced by PRP and ADSC therapies in photoaged skin. Methods. A prospective randomized study involving 20 healthy individuals, showing skin aging. They underwent two therapeutic protocols (protocol 1: PRP; protocol 2: ADSCs). Biopsies were obtained before and after treatment (4 months). Results. PRP protocol showed unwanted changes in the reticular dermis, mainly due to the deposition of a horizontal layer of collagen (fibrosis) and elastic fibers tightly linked. Structural analyses revealed infiltration of mononuclear cells and depot of fibrotic material in the reticular dermis. The ADSC protocol leads to neoelastogenesis with increase of tropoelastin and fibrillin. There was an improvement of solar elastosis inducing an increment of macrophage polarization and matrix proteinases. These last effects are probably related to the increase of elastinolysis and the remodeling of the dermis. Conclusions. The PRP promoted an inflammatory process with an increase of reticular dermis thickness with a fibrotic aspect. On the other hand, ADSC therapy is a promising modality with an important antiaging effect on photoaged human skin.

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“…prp injection (12) in the intestinal wall has more advantages in comparison with the soaking method. Histology did not reveal any fibrosis growth in the injection area, any microangiopathy, and inflammatory process in the 2 nd group of the laboratory animals.…”

Section: Discussionmentioning

confidence: 99%

Platelet-rich autoplazma effect on intestinal anastomosis regeneration in rabbits

Nuraddinovich-Dzhumabekov

Tulegenovich-Dzhumabekov

Kazybaevich-Ismailov

et al. 2020

lib oncol

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Background: the failure of the intestinal anastomosis remains one of the main problems in gastrointestinal surgery. new principles for solving this problem are focused on the cellular level of regeneration using platelet-rich plasma (prp). In this study, we evaluated the effect of PRP and experimentally compared the effectiveness of various application methods in intestinal anastomosis regeneration in rabbits. Materials and Methods: platelet-rich autoplasma was obtained from 81 rabbits, which we used to study two methods of applying prp (injection into the muscle layer/soaking in the intestinal wall) in comparison with the control group on the end-to-end small-intestinal anastomosis. We performed a morphological study of intestinal anastomoses after 24, 48, 72 hours, and after 7 days. We evaluated the deformation and strength characteristics of the anastomosis on the 7th day. Results: analysis of the histological and morphometric data of the study showed that the regeneration between the samples of intestinal anastomosis treated with prp soaking and injections into the intestinal wall is more favorable in comparison with the control group. The criteria were the quantitative ratio of inflammatory infiltrate, fibroblast proliferation, neoangiogenesis, and collagen deposition. PRP soaking in the intestinal wall significantly increased the number of adhesion conglomerates, which contributed to severe deformation and narrowing of more than ½ of the lumen in the intestinal tube. Analysis of the deformation and strength characteristics of the formed anastomoses also showed significantly higher values of the rupture strength of the anastomoses treated with prp, in contrast to the control group. Conclusion: PRP soaking and injection into the intestinal wall can have a positive effect on the regeneration of the intestinal anastomosis. PRP injection into the muscular layer of the intestinal wall significantly reduces the development of adhesions in comparison with the prp-saturated group of intestinal anastomoses. further research is needed to clarify the optimal method of prp application, which will improve the regeneration of the intestinal anastomosis.

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Effect of Use of Platelet-Rich Plasma (PRP) in Skin with Intrinsic Aging Process

Cited by 38 publications

References 19 publications

Effects of autologous platelet‑rich plasma injections on facial skin rejuvenation

Effects of autologous platelet‑rich plasma injections on facial skin rejuvenation

Photoaging Skin Therapy with PRP and ADSC: A Comparative Study

Platelet-rich autoplazma effect on intestinal anastomosis regeneration in rabbits

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