Effect of unilateral testicular torsion on contralateral testis in a rat model

Xia, Zhaobo; Hu, Jun; Han, Lei; Xia, Qiongzhang; Shao, Fangbin; Lin, Xiaokun

doi:10.1007/s00383-020-04626-y

Cited by 10 publications

(12 citation statements)

References 21 publications

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“…The possible mechanisms include decreased blood flow, acrosomal enzyme release, stimulated sympathetic response, or immunological responses of the spermatogonium. 4,[38][39][40] Consistently, the present study showed that unilateral testicular I/R injury caused significant contralateral testicular damage, for example, contralateral testis swelling, disorganized spermatogenesis, and cell apoptosis. However, both liver and limb postconditioning successfully ameliorated the deterioration of the contralateral testis, suggesting that the RLIPost strategy is capable of protecting both ipsilateral and contralateral testes when unilateral testicular torsion/detorsion occurs.…”

Section: Discussionsupporting

confidence: 87%

Protective effect of remote ischemic postconditioning in rat testes after testicular torsion/detorsion

Liu

Yan

et al. 2022

Andrology

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Background: Testicular torsion/detorsion can lead to severe testicular damage.The organ-protective effect of remote ischemic postconditioning (RLIPost) against ischemia/reperfusion injury has been characterized; however, it remains unknown whether RLIPost has a testicular protective effect.Objectives: Here, we tested the hypothesis that RLIPost can protect the testes in a rat model of testicular torsion/detorsion in vivo. Materials and methods:Male Sprague-Dawley rats were assigned to sham-operated, control, or remote liver and limb ischemic postconditioning-treated groups. Testicular torsion/detorsion was performed by 3 h of testicular torsion (720 • clockwise unilateral spermatic cord torsion), followed by 3 h of detorsion. For liver and limb ischemic postconditioning, four cycles of 5 min of liver or limb ischemia with 5-min intermittent reperfusion stimuli were conducted at the onset of testicular reperfusion.Results: Liver and limb ischemic postconditioning significantly ameliorated ipsilateral and contralateral testicular swelling responses, preserved morphological integrity and spermatogenesis and inhibited testicular apoptosis. In addition, RLIPost enhanced the phosphorylation of AKT/ERK1/2/GSK-3β/STAT-3 in the ipsilateral testis while suppressing JNK activation in the ipsilateral and contralateral testes. Discussion and conclusion:To the best of our knowledge, this study is the first to demonstrate the involvement of RLIPost in an animal model of testicular torsion/detorsion. We showed that RLIPost protects both ipsilateral and contralateral testes against testicular torsion/detorsion in vivo, via at least in part, the RISK and SAFE-mediated signaling pathways.

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Section: Discussionsupporting

confidence: 87%

Protective effect of remote ischemic postconditioning in rat testes after testicular torsion/detorsion

Liu

Yan

et al. 2022

Andrology

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“…Debate continues regarding whether contralateral testicular damage occurs after unilateral testicular ischemia-reperfusion. Some studies reported that contralateral testis was affected by unilateral testicular ischemia-reperfusion [ 71 – 74 ], but other studies showed no changes in the contralateral testis [ 75 – 77 ]. In our study, although unilateral testicular ischemia-reperfusion led to significant changes in malondialdehyde content, superoxide dismutase and catalase protein expression, and spermatogenesis in ipsilateral testis, it had no effect on contralateral testis (Figures 1 – 4 ).…”

Section: Discussionmentioning

confidence: 99%

Salidroside Exerts Beneficial Effect on Testicular Ischemia-Reperfusion Injury in Rats

Wei

Huang

Qin

2022

Oxidative Medicine and Cellular Longevity

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Testicular torsion-detorsion results in testicular ischemia-reperfusion injury, which is associated with overgeneration of reactive oxygen species. Salidroside, a major bioactive ingredient extracted from Rhodiola rosea, has strong antioxidant activity. The purpose of this study was to examine the effect of salidroside on testicular ischemia-reperfusion injury. Sixty rats were randomly separated into 3 experimental groups: group A = sham-operated control; group B = testicular ischemia-reperfusion; and group C = testicular ischemia-reperfusion treated with salidroside. The rats in the sham-operated control group received all surgical procedures except testicular torsion-detorsion. The testicular ischemia-reperfusion group underwent 2 hours of left testicular torsion followed by detorsion. The rats in the salidroside-treated group received the same surgical procedure as in testicular ischemia-reperfusion group, but salidroside was injected intraperitoneally at reperfusion. Testicular malondialdehyde content (a reliable index of reactive oxygen species) and protein expression of superoxide dismutase and catalase which are primary antioxidant enzymes in testes were measured at 4 hours after reperfusion. Testicular spermatogenesis was evaluated at 3 months after reperfusion. The malondialdehyde content increased significantly, while superoxide dismutase and catalase protein expression and testicular spermatogenesis reduced significantly in ipsilateral testes of testicular ischemia-reperfusion group, as compared with sham-operated control group. Therapy with salidroside significantly reduced malondialdehyde content and significantly enhanced superoxide dismutase and catalase protein expression and spermatogenesis in ipsilateral testes, as compared with testicular ischemia-reperfusion group. The present findings indicate that treatment with salidroside ameliorates testicular ischemia-reperfusion injury by reducing reactive oxygen species level by upregulating superoxide dismutase and catalase protein expression.

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“…Irreversible damage starts after four to six hours and affects both the spermatogenesis and the endocrine functions 15,16 . Also, it has been suggested that unilateral testicular torsion may harm the spermatogenesis in the contralateral testis through ischemia-reperfusion metabolites as an early mechanism and by autoimmune response in latter stages 17 . A study comparing the time to treat and duration of symptoms in respect of testicular salvage indicates a clear significance in conserving the testis in the group with duration of symptoms below 24 hours while there was no significance in time to treat 18 .…”

Section: Discussionmentioning

confidence: 99%

The Impact of COVID-19 Pandemic on Admission Times, Manual Detorsion Rates and Outcomes in Testicular Torsion

Tokuç

Artuk

Kayar

et al. 2021

Preprint

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Aim: This study aims to assess the admission times, manual detorsion rates and outcomes of the testicular torsion cases during COVID-19 pandemic. Methods: A retrospective analysis of the testicular torsion cases was done through our hospital’s electronic archive. Patients with were distributed into two groups as “COVID-19 era” (between 11th of March 2020 – March 2021) and “pre-COVID-19 era” (between January 2018 and 10th of March 2020). Time between the onset of symptoms and the moment of detorsion was calculated as the duration of ischemia. Two groups were compared in terms of ischemia duration, manual detorsion rates and operational outcomes. Results: No statistically significant differences was detected between groups in terms of ischemia duration, manual detorsion rates and surgical procedure type (all p>0.05). During the COVID-19 pandemic, the number and percentage of manual detorsions have increased, resulting in a numerical decrease in orchiectomies. Conclusions: Conditions caused by COVID-19 may prompt urologists to manual detorsion more to set up safer surgical environment. Moreover, this approach may result in a decrease in orchiectomy rates by saving time and preserving the vascularity of the testis. Further studies with larger samples sizes and meta-analyses may support this concept. Keywords: testicular torsion, COVID-19, manual detorsion, orchiectomy, emergency

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Effect of unilateral testicular torsion on contralateral testis in a rat model

Cited by 10 publications

References 21 publications

Protective effect of remote ischemic postconditioning in rat testes after testicular torsion/detorsion

Protective effect of remote ischemic postconditioning in rat testes after testicular torsion/detorsion

Salidroside Exerts Beneficial Effect on Testicular Ischemia-Reperfusion Injury in Rats

The Impact of COVID-19 Pandemic on Admission Times, Manual Detorsion Rates and Outcomes in Testicular Torsion

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