Protective effect of remote ischemic postconditioning in rat testes after testicular torsion/detorsion

Hu, Zhaoyang; Liu, Quanhua; Yan, Zhe; Wang, Qifeng; Liu, Jin

doi:10.1111/andr.13184

Cited by 8 publications

(5 citation statements)

References 49 publications

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“…In this study, we investigated the potential protective effects of carvacrol on testicular injury induced by T/D in a rat model. Our findings revealed significant alterations in various parameters, including histopathological changes, oxidative stress markers, and serum testosterone levels, highlighting the potential therapeutic role of carvacrol in mitigating testicular damage (12,13). Histopathological analysis plays a crucial role in assessing tissue damage and pathological changes (14).…”

Section: Discussionmentioning

confidence: 64%

Preservation of Testicular Tissue and Alleviation of Oxidative Stress by Carvacrol Following Torsion/ Detorsion in Adult Male Rats

Khatami,

Rahmanifar,

Khaki

et al. 2023

Crescent J Med Biol Sci

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Objectives: Testicular torsion is a critical medical condition necessitating prompt diagnosis and intervention. This study aimed to explore the potential safeguarding effect of carvacrol against histological damage and oxidative stress resulting from torsion/detorsion (T/D) in rat testes. Materials and Methods: A total of 32 adult male rats were randomly divided into four groups. The sham group did not undergo any intervention. The second group received an intraperitoneal injection of 75 mg/kg carvacrol half an hour before detorsion application. The third group was administered 80 mg/kg carvacrol intraperitoneally without detorsion. The fourth group (control) experienced (T/D) through the administration of saline. Following a 5-hour reperfusion period, the left testis was excised for histological slide preparation. Blood serum was used to measure antioxidant enzyme levels. Data analysis was performed using the SPSS version 19 software and analysis of variance tests. Results: Significant histological alterations were observed between the sham and other three groups. The levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), and testosterone significantly decreased in all treatment groups compared to the control group (P<0.05). Conversely, malondialdehyde (MDA) levels increased in the torsion control group in contrast to the sham group (P<0.05). Carvacrol administration mitigated MDA levels in the treatment groups. Also, there were significant differences in tissue parameters between the sham and the other groups (P<0.05). Conclusions: According to the results of this study, carvacrol possesses the potential to mitigate testicular tissue damage, enhance testicular function, and ameliorate oxidative stress consequential to testicular rotation.

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Section: Discussionmentioning

confidence: 64%

Preservation of Testicular Tissue and Alleviation of Oxidative Stress by Carvacrol Following Torsion/ Detorsion in Adult Male Rats

Khatami,

Rahmanifar,

Khaki

et al. 2023

Crescent J Med Biol Sci

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“…It is worth mentioning that we previously found that remote ischemic conditioning executed after testicular detorsion (RIPost) protected rat testes against testicular I/R injury. However, ERK1/2 phosphorylation was increased only in RIPost-treated ipsilateral testes but not in contralateral testes after detorsion when compared to rats in the non-RIPost-treated testicular I/R group [ 12 ]. Taken together, our results indicate that RIPC and RIPost may protect ipsilateral testis against testicular I/R via activation of ERK1/2.…”

Section: Discussionmentioning

confidence: 99%

“…Testicular torsion and detorsion were performed under anesthesia with sodium pentobarbital (50 mg/kg, i.p.) [ 11 , 12 ]. Testicular torsion/detorsion surgerical procedure was performed based on previously publications [ 6 , 12 ].…”

Section: Methodsmentioning

confidence: 99%

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Pretreatment with remote ischemic conditioning attenuates testicular damage after testicular ischemia and reperfusion injury in rats

Li,

Yan,

Wang

et al. 2023

PLoS ONE

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Testicular torsion is a urological emergency. However, surgical detorsion of the torsed spermatic cord can cause testicular reperfusion injury. Although remote ischemic preconditioning (RIPC) has been convincingly shown to protect organs against ischemia/reperfusion (I/R) injury, little is known regarding the effect of RIPC on testicular torsion/detorsion-induced reperfusion injury. Therefore, we aimed to evaluate the effect of RIPC on testes after testicular I/R injury in a rat model in vivo. Male Sprague–Dawley rats were randomly classified into 4 groups: sham-operated (sham), testicular I/R (TI/R), or remote liver (RIPC liver) and limb (RIPC limb) ischemic preconditioning groups. Testis I/R was induced by 3 h of right spermatic cord torsion (720° clockwise), and reperfusion was allowed for 3 hours. In the RIPC group, four cycles of 5 min of ischemia and 5 min of reperfusion were completed 30 min prior to testicular torsion. The ERK1/2 inhibitor U0126 was administered intravenously at the beginning of reperfusion (1 mg/kg). The testes were taken for the oxidative stress evaluations, histology, apoptosis, immunohistochemical and western blotting analysis. Remote liver and limb ischemic preconditioning attenuated ipsilateral and contralateral testicular damage after testicular I/R injury. For example. RIPC reduced testicular swelling and oxidative stress, lessened structural damage, and inhibited the testicular inflammatory response and apoptosis. Furthermore, RIPC treatment enhanced testicular ERK1/2 phosphorylation postI/R. Inhibition of ERK1/2 activity using U0126 eliminated the protection offered by RIPC. Our data demonstrate for the first time that RIPC protects testes against testicular I/R injury via activation of the ERK1/2 signaling pathway.

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“…The condition of the contralateral untwisted testis is debatable after unilateral testicular ischemia-reperfusion. In some studies, it has been reported that unilateral testicular ischemia-reperfusion induces harmful effect on the contralateral testis [ 51 , 52 ]. In contrast, other studies have indicated that no injury is observed in the contralateral testis [ 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Baicalein Alleviates Testicular Ischemia-Reperfusion Injury in a Rat Model of Testicular Torsion-Detorsion

Wei

Huang

2022

Oxidative Medicine and Cellular Longevity

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Testicular torsion/detorsion-induced ischemia/reperfusion injury is partly due to the overgeneration of reactive oxygen species. Baicalein, a main bioactive constituent derived from the dried root of Scutellaria baicalensis Georgi, possesses powerful antioxidative and anti-inflammatory properties. Therefore, we designed the research to explore the possible protective effect of baicalein against testicular ischemia-reperfusion injury. Sprague-Dawley rats were randomized into 4 groups, including control, testicular ischemia-reperfusion, testicular ischemia-reperfusion+vehicle injection, and testicular ischemia-reperfusion+baicalein therapy groups. The control group received surgical exposure of the left testis without torsion-detorsion. In the testicular ischemia-reperfusion group, the left testis underwent 720° counterclockwise torsion for two hours and then was allowed detorsion. Rats in the testicular ischemia-reperfusion+vehicle injection group received intraperitoneal injection of the vehicle at detorsion. In the baicalein-treated group, the intraperitoneal administration of baicalein dissolved in the vehicle was performed at detorsion. At four hours or three months following testicular detorsion, testicular tissues were removed to detect the levels of tumor necrosis factor-alpha (TNF-α) and interleukin-1beta (IL-1β) which can recruit neutrophils into the testis, myeloperoxidase activity (an index of neutrophil infiltration in the testis), protein expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase in neutrophils which can catalyze reactive oxygen species production, malondialdehyde concentration (a common marker of reactive oxygen species), and spermatogenesis. Both testicular ischemia-reperfusion and testicular ischemia-reperfusion+vehicle injection significantly increased the TNF-α and IL-1β levels, myeloperoxidase activity, NADPH oxidase protein expression, and malondialdehyde concentration, while decreased spermatogenesis in ipsilateral testes. In contrast, baicalein administration remarkably reduced TNF-α and IL-1β levels, myeloperoxidase activity, NADPH oxidase protein expression, and malondialdehyde concentration and also elevated spermatogenesis in ipsilateral testes. The results of our experiment demonstrate that baicalein alleviates testicular ischemia-reperfusion injury by inhibiting TNF-α and IL-1β secretion, neutrophil infiltration in the testis, and NADPH oxidase protein expression in neutrophils to reduce reactive oxygen species production.

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Protective effect of remote ischemic postconditioning in rat testes after testicular torsion/detorsion

Cited by 8 publications

References 49 publications

Preservation of Testicular Tissue and Alleviation of Oxidative Stress by Carvacrol Following Torsion/ Detorsion in Adult Male Rats

Preservation of Testicular Tissue and Alleviation of Oxidative Stress by Carvacrol Following Torsion/ Detorsion in Adult Male Rats

Pretreatment with remote ischemic conditioning attenuates testicular damage after testicular ischemia and reperfusion injury in rats

Baicalein Alleviates Testicular Ischemia-Reperfusion Injury in a Rat Model of Testicular Torsion-Detorsion

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