Effect of unilateral labyrinthectomy on the molecular composition of perineuronal nets in the lateral vestibular nucleus of the rat

Deák, Ádám; Bácskai, Tímea; Gaál, Botond; Rácz, Éva; Matesz, Klára

doi:10.1016/j.neulet.2012.01.076

Cited by 19 publications

(13 citation statements)

References 30 publications

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“…Indeed, enzymatic removal of hyaluronan-based pericellular matrix increases extrasynaptic AMPA receptor diffusion and the exchange of synaptic receptors, modulating short-term synaptic plasticity (Frischknecht et al 2009). Intriguingly, PNN number and staining intensity are restored in coincidence with the compensation of postural vestibular functions (see also Halasi et al 2007;Deák et al 2012). PNN restoration may thus contribute to the stabilisation of the re-wired neuronal connections which may underlie behavioural recovery.…”

Section: Perineuronal Net Changes In the Lvn During Vestibular Compenmentioning

confidence: 96%

“…Previous reports showed reactive neurogenesis and gliogenesis in the ipsilateral VN (De Waele et al 1996;Campos-Torres et al 1999Tighilet et al 2007;Lacour et al 2009), which play a role in functional recovery (Dutheil et al , 2013. Significant changes in the expression of brain-derived neurotrophic factor, choline acetyltransferase, GABA, neuroprotective factors, markers of inflammation and extracellular matrix molecules have been detected in the VN after peripheral damage (Smith et al 1998;Gustave Dit Duflo et al 1999;Lacour 1998, 2001;Lacour and Tighilet 2010;Liberge et al 2010;Deák et al 2012). …”

Section: Introductionmentioning

confidence: 96%

“…For example, exposure to an enriched environment induces significant axonal remodelling in the cerebellar nuclei and enhances visual cortex plasticity, in parallel with a conspicuous reduction of PNNs (Sale et al 2007;Foscarin et al 2011). PNNs are also diminished during injury-induced neuritic remodelling (Carmichael et al 2005;Halasi et al 2007;Deák et al 2012;Carulli et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Modifications of perineuronal nets and remodelling of excitatory and inhibitory afferents during vestibular compensation in the adult mouse

et al. 2015

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Perineuronal nets (PNNs) are aggregates of extracellular matrix molecules surrounding several types of neurons in the adult CNS, which contribute to stabilising neuronal connections. Interestingly, a reduction of PNN number and staining intensity has been observed in conditions associated with plasticity in the adult brain. However, it is not known whether spontaneous PNN changes are functional to plasticity and repair after injury. To address this issue, we investigated PNN expression in the vestibular nuclei of the adult mouse during vestibular compensation, namely the resolution of motor deficits resulting from a unilateral peripheral vestibular lesion. After unilateral labyrinthectomy, we found that PNN number and staining intensity were strongly attenuated in the lateral vestibular nucleus on both sides, in parallel with remodelling of excitatory and inhibitory afferents. Moreover, PNNs were completely restored when vestibular deficits of the mice were abated. Interestingly, in mice with genetically reduced PNNs, vestibular compensation was accelerated. Overall, these results strongly suggest that temporal tuning of PNN expression may be crucial for vestibular compensation.

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Section: Perineuronal Net Changes In the Lvn During Vestibular Compenmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Modifications of perineuronal nets and remodelling of excitatory and inhibitory afferents during vestibular compensation in the adult mouse

et al. 2015

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“…While WFA intensity is important for formulating experiments and interpreting the findings, the method by which WFA intensity is measured and analyzed has been inconsistent across studies. Some studies have used semi-quantitative methods to examine relative intensity changes, but these studies lack measures of individual PNNs (Wang et al, 2011, Deak et al, 2012, Yamada and Jinno, 2013, Racz et al, 2014, Kecskes et al, 2015). Other studies examine changes in individual PNNs by averaging the intensity of 15 pixels within a given PNN around the soma (Foscarin et al, 2011, Carulli et al, 2013, Vazquez-Sanroman et al, 2015a), and others have not included details in the methodology (Chen et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

A standardized and automated method of perineuronal net analysis using Wisteria floribunda agglutinin staining intensity

2016

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Perineuronal nets (PNNs) are aggregations of extracellular matrix molecules that are critical for plasticity. Their altered development or changes during adulthood appear to contribute to a wide range of diseases/disorders of the brain. An increasing number of studies examining the contribution of PNN to various behaviors and types of plasticity have analyzed the fluorescence intensity of Wisteria floribunda agglutinin (WFA) as an indirect measure of the maturity of PNNs, with brighter WFA staining corresponding to a more mature PNN and dim WFA staining corresponding to an immature PNN. However, a clearly-defined and unified method for assessing the intensity of PNNs is critical to allow us to make comparisons across studies and to advance our understanding of how PNN plasticity contributes to normal brain function and brain disease states. Here we examined methods of PNN intensity quantification and demonstrate that creating a region of interest around each PNN and subtracting appropriate background is a viable method for PNN intensity quantification that can be automated. This method produces less variability and bias across experiments compared to other published analyses, and this method increases reproducibility and reliability of PNN intensity measures, which is critical for comparisons across studies in this emerging field.

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“…Most surgical techniques reported until now include extensive extirpation of the wall of the meatus acusticus externus and structures of middle ear (1,4,7,9,12,15,16,22,25). The specific anatomy of the rodent skull, however, provides an alternative surgical approach through the tympanic bulla (6,10,11,13). No publication, however, is available, with the exception of Hitier et al (11) with a sufficiently detailed description on how to expose the rat vestibular cavity surgically, while inducing minimum damage to the surrounding sensitive neck, temporal, and skull structures.…”

Section: Introductionmentioning

confidence: 99%

A simple standard technique for labyrinthectomy in the rat: A methodical communication with a detailed description of the surgical process

Nádasy

Raffai

Fehér

et al. 2016

Physiology International

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Aims: Labyrinthectomized rats are suitable models to test consequences of vestibular lesion and are widely used to study neural plasticity. We describe a combined microsurgical-chemical technique that can be routinely performed with minimum damage. Methods: Caudal leaflet of the parotis is elevated. The tendinous fascia covering the bulla is opened frontally from the sternomastoid muscle's tendon while sparing facial nerve branches. A 4 mm diameter hole is drilled into the bulla's hind lower lateral wall to open the common (in rodents) mastoid-tympanic cavity. The cochlear crista (promontory) at the lower posterior part of its medial wall is identified as a bony prominence. A 1 mm diameter hole is drilled into its lower part. The perilymphatic/endolymphatic fluids with tissue debris of the Corti organ are suctioned. Ethanol is injected into the hole. Finally, 10 μL of sodium arsenite solution (50 μM/mL) is pumped into the labyrinth and left in place for 15 min. Simple closure in two layers (fascia and skin) is sufficient. Results and conclusion: All rats had neurological symptoms specific for labyrinthectomy (muscle tone, body position, rotatory movements, nystagmus, central deafness). Otherwise, their behavior was unaffected, drinking and eating normally. After a few days, they learned to balance relying on visual and somatic stimuli (neuroplasticity).

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Effect of unilateral labyrinthectomy on the molecular composition of perineuronal nets in the lateral vestibular nucleus of the rat

Cited by 19 publications

References 30 publications

Modifications of perineuronal nets and remodelling of excitatory and inhibitory afferents during vestibular compensation in the adult mouse

Modifications of perineuronal nets and remodelling of excitatory and inhibitory afferents during vestibular compensation in the adult mouse

A standardized and automated method of perineuronal net analysis using Wisteria floribunda agglutinin staining intensity

A simple standard technique for labyrinthectomy in the rat: A methodical communication with a detailed description of the surgical process

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