Effect of ultraviolet radiation on spore viability and mosquitocidal activity of an indigenous ISPC-8 Bacillus sphaericus Neide strain

Hadapad, Ashok B.; Vijayalakshmi, N.; Hire, Ramesh S.; Dongre, T. K.

doi:10.1016/j.actatropica.2008.04.024

Cited by 20 publications

(20 citation statements)

References 14 publications

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“…Barga et al (6) reported that the viability of the entomopathogenic fungus Verticillium lecanii declined to zero after a 4-h exposure to artificial sunlight. Hadapad et al (18) reported a complete loss in the viability of Bacillus sphaericus spores after 6 h of exposure to artificial sunlight. Using another Bacillus mutant strain, Riesenman and Nicholson (38) documented a relatively high LD 90 value for UV-B exposure (ranging from 28 to 31 Kj/m 2 ).…”

Section: Discussionmentioning

confidence: 99%

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Control of Apple Blue Mold by the Antagonistic YeastPichia anomalaStrain K: Screening of UV Protectants for Preharvest Application

2011

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Lahlali, R., Brostaux, Y., and Jijakli, M. H. 2011. Control of apple blue mold by the antagonistic yeast Pichia anomala strain K: Screening of UV protectants for preharvest application. Plant Dis. 95:311-316.When applied preharvest, antagonistic yeasts that act as biocontrol agents of postharvest fruit diseases must survive the environmental conditions in the field. In particular, UV-B radiation (280 to 320 nm) can greatly reduce their survival and effectiveness. The influence of artificial UV-B radiation on Pichia anomala strain K, an antagonistic yeast with potential for control of postharvest fruit diseases, was evaluated in vitro and in vivo. The in vitro 50 and 90% lethal dose values were 0.89 and 1.6 Kj/m 2 , respectively, whereas lethal values in vivo were 3.2 and 5.76 Kj/m 2 , respectively. UV protectants tested in combination with strain K included congo red, tryptophan, riboflavin, lignin, casein, gelatine, folic acid, tyrosine, and four mixtures. Riboflavin, folic acid, and the mixtures 1% folic acid + 0.5% tyrosine + 0.5% riboflavin (formula 2), 0.5% folic acid + 1% tyrosine + 0.5% riboflavin (formula 3), and 0.5% folic acid + 0.5% tyrosine + 1% riboflavin (formula 4) reduced yeast mortality caused by UV-B radiation in petri dish assays. Riboflavin, folic acid, gelatine, lignin, and tyrosine reduced yeast mortality caused by UV-B radiation on apple fruit surfaces. With the exception of lignin and folic acid, none of the compounds or mixtures increased significantly the ability of strain K to control the postharvest pathogen Penicillium expansum on wounded apple fruit. In contrast, casein, gelatine, tyrosine, congo red, riboflavin, and formulas 1 to 4 significantly reduced the effectiveness of strain K. Further investigations are justified to verify a potential benefit of lignin and folic acid for UV protection of strain K in preharvest applications.

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Section: Discussionmentioning

confidence: 99%

“…Such UV protectants include riboflavin, para-aminobenzoic acid, ascorbic acid, folic acid, uric acid, casein, tyrosine, gelatine, and lignin (2,11,15,17,18,(21)(22)(23)27,42). To the best of our knowledge, there are no reports about the influence of UV-B radiation on the biocontrol agent's survival when applied preharvest for postharvest disease management.…”

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confidence: 89%

Control of Apple Blue Mold by the Antagonistic YeastPichia anomalaStrain K: Screening of UV Protectants for Preharvest Application

2011

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“…In earlier study, we observed the adverse effects of UV-B radiation on spore viability as well as larvicidal activity of B. sphaericus strains (Hadapad et al, 2008). It is essential to incorporate an effective solar protectant to protect the spore viability and larvicidal activity of B. sphaericus.…”

Section: Introductionmentioning

confidence: 96%

UV protectants for the biopesticide based on Bacillus sphaericus Neide and their role in protecting the binary toxins from UV radiation

Hadapad

Hire

Vijayalakshmi

et al. 2009

Journal of Invertebrate Pathology

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“…Photoinactivation (UV-B, 280-320 nm) is one of the major environmental abiotic factors affecting stability of B. sphaericus toxins. The insecticidal activity of unformulated entomopathogens is quickly inactivated by UV radiation (Mulligan et al 1980;Shapiro 1989;Inglis et al 1995;Manasherob et al 2002;Brar et al 2006;Hadapad et al 2008). In this study, complete loss of spore viability and larvicidal activity was observed when the spore-crystal suspension of B. sphaericus ISPC-8 alone was exposed to UV-B (315 nm) for 24 h. Myasnik et al (2001) have also reported reduced viability of B. sphaericus exposed to UV-B radiation for 90 min.…”

Section: Discussionmentioning

confidence: 48%

Sustained-release biopolymer based formulations for Bacillus sphaericus Neide ISPC-8

et al. 2011

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Sustained-release biopolymer based formulations for an indigenous Bacillus sphaericus Neide ISPC-8 strain were developed and evaluated under laboratory conditions. These formulations were assessed for residual activity, response to UV-B radiation and shelf-life. The mosquitocidal activity of tested formulations showed a varied residual activity pattern against Culex quinquefasciatus Say larvae. The residual activity of unformulated spore-crystal suspension drastically decreased after 4 days whereas the formulated preparations persisted until 28 days. All the formulations except sodium alginate-based formulation showed more than 95% mortality within 48 h. Although the sodium alginate-based formulation showed delayed initial mortality, it persisted for a longer period of time (42 days). Based on the persistence studies, promising formulations based on sodium alginate, gelatin and acacia gum were selected for further evaluation. The respective LC 50 values of these three formulations were 0.085, 0.117, and 0.196 mg/l against C. quinquefasciatus after 48 h. The biopolymers combined with Congo red protected the sporecrystal moiety from UV-B radiation. These formulations were found to be stable up to 18 months when stored at room temperature. Thus, these biopolymers could serve as suitable ingredients for development of biopesticide based on B. sphaericus ISPC-8.

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Effect of ultraviolet radiation on spore viability and mosquitocidal activity of an indigenous ISPC-8 Bacillus sphaericus Neide strain

Cited by 20 publications

References 14 publications

Control of Apple Blue Mold by the Antagonistic YeastPichia anomalaStrain K: Screening of UV Protectants for Preharvest Application

Control of Apple Blue Mold by the Antagonistic YeastPichia anomalaStrain K: Screening of UV Protectants for Preharvest Application

UV protectants for the biopesticide based on Bacillus sphaericus Neide and their role in protecting the binary toxins from UV radiation

Sustained-release biopolymer based formulations for Bacillus sphaericus Neide ISPC-8

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