UV protectants for the biopesticide based on Bacillus sphaericus Neide and their role in protecting the binary toxins from UV radiation

Hadapad, Ashok B.; Hire, Ramesh S.; Vijayalakshmi, N.; Dongre, T. K.

doi:10.1016/j.jip.2008.12.003

Cited by 19 publications

(18 citation statements)

References 24 publications

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“…The 10% sodium hypochlorite treatment or damages induced by UV-B radiation stimulus may have altered or removed the natural cuticular waxes from the upper apple fruit, which may have increased the absorption of UV protectants into the cuticle (depending on molecular weight). Hadapad et al (17) found a great variability in the viability of Bacillus sp. spores when treated with UV protectants; they also reported that riboflavin and congo red were more effective than folic acid and gelatine.…”

Section: Discussionmentioning

confidence: 99%

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Control of Apple Blue Mold by the Antagonistic YeastPichia anomalaStrain K: Screening of UV Protectants for Preharvest Application

2011

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Lahlali, R., Brostaux, Y., and Jijakli, M. H. 2011. Control of apple blue mold by the antagonistic yeast Pichia anomala strain K: Screening of UV protectants for preharvest application. Plant Dis. 95:311-316.When applied preharvest, antagonistic yeasts that act as biocontrol agents of postharvest fruit diseases must survive the environmental conditions in the field. In particular, UV-B radiation (280 to 320 nm) can greatly reduce their survival and effectiveness. The influence of artificial UV-B radiation on Pichia anomala strain K, an antagonistic yeast with potential for control of postharvest fruit diseases, was evaluated in vitro and in vivo. The in vitro 50 and 90% lethal dose values were 0.89 and 1.6 Kj/m 2 , respectively, whereas lethal values in vivo were 3.2 and 5.76 Kj/m 2 , respectively. UV protectants tested in combination with strain K included congo red, tryptophan, riboflavin, lignin, casein, gelatine, folic acid, tyrosine, and four mixtures. Riboflavin, folic acid, and the mixtures 1% folic acid + 0.5% tyrosine + 0.5% riboflavin (formula 2), 0.5% folic acid + 1% tyrosine + 0.5% riboflavin (formula 3), and 0.5% folic acid + 0.5% tyrosine + 1% riboflavin (formula 4) reduced yeast mortality caused by UV-B radiation in petri dish assays. Riboflavin, folic acid, gelatine, lignin, and tyrosine reduced yeast mortality caused by UV-B radiation on apple fruit surfaces. With the exception of lignin and folic acid, none of the compounds or mixtures increased significantly the ability of strain K to control the postharvest pathogen Penicillium expansum on wounded apple fruit. In contrast, casein, gelatine, tyrosine, congo red, riboflavin, and formulas 1 to 4 significantly reduced the effectiveness of strain K. Further investigations are justified to verify a potential benefit of lignin and folic acid for UV protection of strain K in preharvest applications.

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Section: Discussionmentioning

confidence: 99%

“…Such UV protectants include riboflavin, para-aminobenzoic acid, ascorbic acid, folic acid, uric acid, casein, tyrosine, gelatine, and lignin (2,11,15,17,18,(21)(22)(23)27,42). To the best of our knowledge, there are no reports about the influence of UV-B radiation on the biocontrol agent's survival when applied preharvest for postharvest disease management.…”

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confidence: 99%

Control of Apple Blue Mold by the Antagonistic YeastPichia anomalaStrain K: Screening of UV Protectants for Preharvest Application

2011

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“…, 1999; Ippolito & Nigro, 2000; Magan, 2001). Various wavelengths of sunlight have been reported to be responsible for photodegradation and inactivation of various microbial fungicides (Hadapad et al. , 2009).…”

Section: Discussionmentioning

confidence: 99%

UV protectants for Candida oleophila (strain O), a biocontrol agent of postharvest fruit diseases

Lahlali¹,

Raffaele²,

Jijakli³

2010

Plant Pathology

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This study investigated the influence of UV-B radiation (280-320 nm) on survival of Candida oleophila strain O, an antagonist yeast that prevents postharvest diseases caused by Botrytis cinerea and Penicillium expansum on apple and pear fruits. Lethal doses (LD 50 and LD 90 ) were, respectively, 0AE89 and 1AE45 Kj m )2 for in vitro exposure and 3AE06 and 5AE5 Kj m )2 for in vivo exposure. A screening test of UV-B protectants for strain O was also evaluated under in vitro and in vivo conditions. The in vitro results showed that sodium ascorbate (0AE1% and 0AE01%), riboflavin (0AE1%) and uric acid (0AE1% and 0AE01%) were the most effective and most suitable protectants. However, only riboflavin (0AE1%) and uric acid (0AE1%) were effective under in vivo conditions. The efficacy obtained with strain O against P. expansum, when subjected to UV-B radiation, was 75AE0% and 49AE2% for pathogen concentrations of 10 5 and 10 6 spores mL, respectively. Adding riboflavin to strain O gave a similar efficacy (64AE2%). Applying strain O together with uric acid (0AE1%) was less active (47AE7%). Nonetheless, its efficacy when applied with the antioxidants sodium ascorbate (71AE1%) or ascorbic acid (82AE5%) was the greatest. Riboflavin and uric acid were the most cost-effective protectants, and could be included in the final formulation of strain O when applied preharvest.

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“…The local strain of B. sphaericus (B. sphaericus ISPC-8) having serotype 5a5b and phage type 3 was isolated from dead larvae of Culex species, collected from field (Hadapad et al, 2009). B. sphaericus was grown in nutrient broth at 28°C till sporulation and pellet obtained was lyophilized and stored in airtight container.…”

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

Purification and characterization of mosquitocidal Bacillus sphaericus BinA protein

Hire

Hadapad

Dongre

et al. 2009

Journal of Invertebrate Pathology

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UV protectants for the biopesticide based on Bacillus sphaericus Neide and their role in protecting the binary toxins from UV radiation

Cited by 19 publications

References 24 publications

Control of Apple Blue Mold by the Antagonistic YeastPichia anomalaStrain K: Screening of UV Protectants for Preharvest Application

Control of Apple Blue Mold by the Antagonistic YeastPichia anomalaStrain K: Screening of UV Protectants for Preharvest Application

UV protectants for Candida oleophila (strain O), a biocontrol agent of postharvest fruit diseases

Purification and characterization of mosquitocidal Bacillus sphaericus BinA protein

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