Effect of type I collagen derived from tilapia scale on odontoblast-like cells

Tang, Jia; Saito, Takashi

doi:10.1007/s13770-014-0114-8

Cited by 10 publications

(7 citation statements)

References 21 publications

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“…ALP measurement is considered as an early marker of osteogenic differentiation whereas OPN and OCN usually reflect the endpoint of osteogenesis in vitro [38]. COL-I is broadly distributed in bone and dentin, which acts as a structural support and biological signal to surrounding cells [39]. In the present study, upregulated odonto/osteogenic markers of both early-stage and late-stage indicated the long term effects of YNB on the committed differentiation of SCAPs.…”

Section: Discussionmentioning

confidence: 59%

Yunnan Baiyao Conditioned Medium Promotes the Odonto/Osteogenic Capacity of Stem Cells from Apical Papilla via Nuclear Factor Kappa B Signaling Pathway

Pang

Wang

et al. 2019

BioMed Research International

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Yunnan Baiyao is a traditional Chinese herbal remedy that has long been used for its characteristics of wound healing, bone regeneration, and anti-inflammation. However, the effects of Yunnan Baiyao on the odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs) and the potential mechanisms remain unclear. The aim of this study was to investigate the odonto/osteogenic differentiation effects of Yunnan Baiyao on SCAPs and the underlying mechanisms involved. SCAPs were isolated and cocultured with Yunnan Baiyao conditioned media. The proliferation ability was determined by cell counting kit 8 and flow cytometry. The differentiation capacity and the involvement of NF-κB pathway were investigated by alkaline phosphatase assay, alizarin red staining, immunofluorescence assay, real-time RT-PCR, and western blot analyses. Yunnan Baiyao conditioned medium at the concentration of 50 μg/mL upregulated alkaline phosphatase activity, induced more mineralized nodules, and increased the expression of odonto/osteogenic genes/proteins (e.g., OCN/OCN, OPN/OPN, OSX/OSX, RUNX2/RUNX2, ALP/ALP, COL-I/COL-I, DMP1, DSP/DSPP) of SCAPs. In addition, the expression of cytoplasmic phos-IκBα, phos-P65, and nuclear P65 was significantly increased in Yunnan Baiyao conditioned medium treated SCAPs in a time-dependent manner. Conversely, the differentiation of Yunnan Baiyao conditioned medium treated SCAPs was obviously inhibited when these stem cells were cocultured with the specific NF-κB inhibitor BMS345541. Yunnan Baiyao can promote the odonto/osteogenic differentiation of SCAPs via the NF-κB signaling pathway.

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Section: Discussionmentioning

confidence: 59%

Yunnan Baiyao Conditioned Medium Promotes the Odonto/Osteogenic Capacity of Stem Cells from Apical Papilla via Nuclear Factor Kappa B Signaling Pathway

Pang

Wang

et al. 2019

BioMed Research International

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“…The mouse dental papilla cell-23 (MDPC-23) cell line was developed as a spontaneously immortalized cell line derived from fetal mouse first molar papillae cells and cloned specifically to have high ALP activity, the ability to form multilayered nodules and a cell doubling time of less than 24 h. MDPC-23 cell line makes transcription products for DSP, type-I collagen, ALP, OPN and osteocalcin (OCN) [ 25 ]. Out of eleven studies selected, two studies used MDPC-23 as their choice of cell type [ 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have characterized the properties of type-I collagen derived from fish scale [ 51 – 53 ], and reported the potential application of as biomaterials in tissue engineering [ 54 – 56 ]. Based on this knowledge, Tang and Saito [ 26 ] investigated the growth, differentiation, mineralization and morphology of MDPC-23 when cultured on tilapia scale collagen (TS collagen). The cell morphology photographs and number suggested that triple helical TS collagen acted as a sticky cue to attract more cells to anchor to the culture plate.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells: A comprehensive methods for odontoblastic induction

et al. 2021

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Background In the area of oral and maxillofacial surgery, regenerative endodontics aims to present alternative options to conventional treatment strategies. With continuous advances in regenerative medicine, the source of cells used for pulp tissue regeneration is not only limited to mesenchymal stem cells as the non-mesenchymal stem cells have shown capabilities too. In this review, we are systematically assessing the recent findings on odontoblastic differentiation induction with scaffold and non-scaffold approaches. Methods A comprehensive search was conducted in Pubmed, and Scopus, and relevant studies published between 2015 and 2020 were selected following the PRISMA guideline. The main inclusion criteria were that articles must be revolving on method for osteoblast differentiation in vitro study. Therefore, in vivo and human or animal clinical studies were excluded. The search outcomes identified all articles containing the word “odontoblast”, “differentiation”, and “mesenchymal stem cell”. Results The literature search identified 99 related studies, but only 11 articles met the inclusion criteria. These include 5 odontoblastic differentiation induction with scaffold, 6 inductions without scaffolds. The data collected were characterised into two main categories: type of cells undergo odontoblastic differentiation, and odontoblastic differentiation techniques using scaffolds or non-scaffold. Conclusion Based on the data analysis, the scaffold-based odontoblastic induction method seems to be a better option compared to the non-scaffold method. In addition of that, the combination of growth factors in scaffold-based methods could possibly enhance the differentiation. Thus, further detailed studies are still required to understand the mechanism and the way to enhance odontoblastic differentiation.

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“…Collagen is the most abundant protein in mammals and it is a key component of the extracellular matrix [23]. Controlling its production is the key element in tissue regeneration.…”

Section: Discussionmentioning

confidence: 99%

Effect of Palmitoyl-Pentapeptide (Pal-KTTKS) on Wound Contractile Process in Relation with Connective Tissue Growth Factor and α-Smooth Muscle Actin Expression

Park

Lee

2017

Tissue Eng Regen Med

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To evaluate whether Palmitoyl-pentapeptide (Pal-KTTKS), a lipidated subfragment of type 1 pro-collagen (residues 212-216), plays a role in fibroblast contractility, the effect of Pal-KTTKS on the expression of pro-fibrotic mediators in hypertropic scarring were investigated in relation with trans-differentiation of fibroblast to myofibroblast, an icon of scar formation. a-SMA was visualized by immunofluorescence confocal microscopy with a Cy-3-conjugated monoclonal antibody. The extent of a-SMA-positive fibroblasts was determined in collagen lattices and in cell culture study. Pal-KTTKS (0-0.5 lM) induced CTGF and a-SMA protein levels were determined by western blot analysis and fibroblast contractility was assessed in three-dimensional collagen lattice contraction assay. In confocal analysis, fibroblasts were observed as elongated and spindle shapes while myofibroblast observed as squamous, enlarged cells with pronounced stress fibers. Without Pal-KTTKS treatment, three quarters of the fibroblasts differentiates into the myofibroblast; a-SMApositive stress fibers per field decreased twofold with 0.1 lM Pal-KTTKS treatment (75 ± 7.1 vs 38.6 ± 16.1%, n = 3, p \ 0.05). The inhibitory effect was not significant in 0.5 lM Pal-KTTKS treatment. Stress fiber level and collagen contractility correlates with a-SMA expression level. In conclusion, Pal-KTTKS (0.1 lM) reduces a-SMA expression and trans-differentiation of fibroblasts to myofibroblast. The degree of reduction is dose-dependent. An abundance of myofibroblast and fibrotic scarring is correlated with excessive levels of a-SMA and collagen contractility. Delicate balance between the wound healing properties and pro-fibrotic abilities of pentapeptide KTTKS should be considered for selecting therapeutic dose for scar prevention.

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Effect of type I collagen derived from tilapia scale on odontoblast-like cells

Cited by 10 publications

References 21 publications

Yunnan Baiyao Conditioned Medium Promotes the Odonto/Osteogenic Capacity of Stem Cells from Apical Papilla via Nuclear Factor Kappa B Signaling Pathway

Yunnan Baiyao Conditioned Medium Promotes the Odonto/Osteogenic Capacity of Stem Cells from Apical Papilla via Nuclear Factor Kappa B Signaling Pathway

Mesenchymal stem cells: A comprehensive methods for odontoblastic induction

Effect of Palmitoyl-Pentapeptide (Pal-KTTKS) on Wound Contractile Process in Relation with Connective Tissue Growth Factor and α-Smooth Muscle Actin Expression

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