Effect of tumor removal on mucosal protein synthesis  in patients with colorectal cancer

Rittler, P.; Demmelmair, Hans; Koletzko, Berthold; Jauch, Karl‐Walter; Hartl, Wolfgang H.

doi:10.1152/ajpendo.00474.2002

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…We also evaluated the rate of protein synthesis in colonic tissue by using stable isotopically labeled tracer infusion to provide an additional and independent marker of cellular metabolism that is associated with proliferation. Several previous studies have used isotope tracer infusions to determine colorectal mucosal protein synthetic rate in human subjects during different physiological (e.g., glucagon infusion) and clinical (inflammatory bowel disease, colorectal cancer) conditions (11,13,26). However, the present study is the first to use this technique to evaluate the effect of dietary fiber on colonic protein metabolism.…”

Section: Discussionmentioning

confidence: 95%

Dietary fiber decreases colonic epithelial cell proliferation and protein synthetic rates in human subjects

Weerasooriya¹,

Rennie²,

Anant³

et al. 2006

American Journal of Physiology-Endocrinology and Metabolism

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. Dietary fiber decreases colonic epithelial cell proliferation and protein synthetic rates in human subjects. Am J Physiol Endocrinol Metab 290: E1104 -E1108, 2006; doi:10.1152/ajpendo.00557.2005.-Although it has been proposed that high fiber consumption can prevent proliferative diseases of the colon, the clinical data to support this hypothesis have been inconsistent. To provide a more robust measure of the effects of fiber on colonic mucosal growth than previous studies, we evaluated both cell proliferation and colonic mucosal protein synthesis in nine healthy volunteers after they consumed a typical Western diet (Ͻ20 g fiber/day) or a Western diet supplemented with wheat bran (24 g/day) in a randomized crossover design. Biopsies taken from the sigmoid colon were used to assess mucosal proliferation by determining proliferating cell nuclear antigen (PCNA) in crypt cells and to assess mucosal protein synthetic rate using stable isotopically labeled leucine infusion. Fiber supplementation produced a 12% decrease in labeling index (%crypt cells stained with PCNA) (P Ͻ 0.001) and an 11% decrease in mucosal protein fractional synthetic rate (FSR; P Ͻ 0.05). Moreover, mucosal protein FSR correlated directly with labeling index (r 2 ϭ 0.22, P Ͻ 0.05). These data demonstrate that increased wheat bran consumption decreases colonic mucosal proliferation and support the potential importance of dietary fiber in preventing proliferative diseases of the colon. metabolism; cancer; short-chain fatty acids MORE THAN 35 YEARS AGO, Burkitt (6) hypothesized that dietary fiber can prevent proliferative diseases of the colon, such as colorectal cancer and polyposis syndromes. However, the effect of fiber intake on colonic disease is unclear because of conflicting results from different cross-sectional epidemiologic studies, longitudinal cohort studies, and randomized controlled trials (30,14). In addition, data from studies that evaluated colonocyte proliferation, a presumed marker of colorectal polyp and cancer risk, found dietary fiber or its metabolites [i.e., short-chain fatty acids (SCFAs)] increased (7,8,19,23), decreased (1, 10, 17), or did not change proliferation rates (2, 9).Several factors have been proposed to explain the inconsistent relationship reported between fiber intake and colonic disease, including heterogeneity of dietary fiber, use of inadequate amounts of fiber to affect cellular metabolism, genetic differences in risk of polyps or colon cancer in study subjects, and limitations in the accuracy and precision of techniques used to measure colonocyte proliferation (14,15,32). Therefore, additional studies that address the limitations of previous investigations are needed to evaluate the effect of fiber on colon proliferation and metabolism. This issue has considerable physiological and clinical importance because of the notion that a typical Western diet, which contains Ͻ15 g/day of nonstarch polysaccharides, is considered inadequate for optimal for colonic health (15, 16).The purpose of the present study wa...

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Section: Discussionmentioning

confidence: 95%

Dietary fiber decreases colonic epithelial cell proliferation and protein synthetic rates in human subjects

Weerasooriya¹,

Rennie²,

Anant³

et al. 2006

American Journal of Physiology-Endocrinology and Metabolism

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An integrative approach to in-vivo protein synthesis measurement: from whole tissue to specific proteins

Guillet

Boirie‌

Walrand

2004

Current Opinion in Clinical Nutrition and Metabolic Care

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To understand the mechanisms by which protein metabolism is altered during physiopathological situations, it is of importance to measure the effect on specific proteins rather than on the body as a whole. Procedures are currently under development with the aim of isolating individuals proteins and to measure their synthesis rates by isotopic methods. Such technical progress is needed to gain a better understanding of the regulation of protein metabolism in situations in which loss of body protein mass occurs.

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Stable Isotope Analysis of the Bioelements: An Introduction

Flenker

2009

Bioanalysis

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The abundances of the stable isotopes of the bioelements are not constant. Subtle, but significant, variations may be induced by physical, physiological and biochemical processes. These variations may be detected and quantified. Often, isotope fingerprints are characteristic of certain processes and may reveal information concerning the sources and origins of compounds of interest. Moreover, natural variabilities of stable isotopes may be exploited in order to perform tracer experiments. The most accurate technology to perform stable isotope analysis is (gas) isotope ratio MS (IRMS). Compound-specific approaches employ hyphenation of GC and LC to IRMS. In these approaches, complete conversion to simple gases prior to MS is required. Analysis by stable isotope ratio spectroscopy currently approaches the accuracy of IRMS. However, for bioanalytical projects, it is still predominantly confined to material synthetically enriched with stable isotopes.

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Effect of tumor removal on mucosal protein synthesis in patients with colorectal cancer

Cited by 3 publications

References 24 publications

Dietary fiber decreases colonic epithelial cell proliferation and protein synthetic rates in human subjects

Dietary fiber decreases colonic epithelial cell proliferation and protein synthetic rates in human subjects

An integrative approach to in-vivo protein synthesis measurement: from whole tissue to specific proteins

Stable Isotope Analysis of the Bioelements: An Introduction

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