Effect of triptolide on estradiol release from cultured rat granulosa cells

Zhang, Juan; Liu, Li; Mu, Xiaoli; Jiang, Zhenzhou; Zhang, Luyong

doi:10.1507/endocrj.ej11-0407

Cited by 27 publications

(21 citation statements)

References 40 publications

(40 reference statements)

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“…Males and females were housed individually for 15 consecutive days (days 1-15). On day 16 of access to bait, control females and males, and treated females and males were housed as individual pairs for mating (treatment-paired, randomly assigned, breeding cycle 1) with continued access to bait (days [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35]. Females and males were separated for 2 wk (rest cycle days 36-50) to allow pups to be born and counted.…”

Section: Wild-caught Ratsmentioning

confidence: 99%

“…Two mechanistic studies in cultured rat granulosa cells reported that triptolide in vitro disrupts the cAMP-dependent steroidogenic pathway, specifically by inhibiting expression of estrogen-synthesizing enzymes. 29,30 Additionally, Tripterygium wilfordii treatment causes infertility in male rats. 8 This infertility was reversible in 40% of treated males.…”

Section: Introductionmentioning

confidence: 99%

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COMPROMISED FERTILITY IN FREE FEEDING OF WILD-CAUGHT NORWAY RATS (RATTUS NORVEGICUS) WITH A LIQUID BAIT CONTAINING 4-VINYLCYCLOHEXENE DIEPOXIDE AND TRIPTOLIDE

Witmer¹,

Raymond-Whish²,

Moulton³

et al. 2017

Journal of Zoo and Wildlife Medicine

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Wild rat pests in the environment cause crop and property damage and carry disease. Traditional methods of reducing populations of these pests involve poisons that can cause accidental exposures in other animals and humans. Fertility management with nonlethal chemicals would be an improved method of rat pest population control. Two chemicals known to target ovarian function in female rats are 4-vinylcyclohexene diepoxide (VCD) and triptolide. Additionally, triptolide impairs spermatogenesis in males. A liquid bait containing no active ingredients (control), or containing triptolide (0.001%) and VCD (0.109%; active) was prepared to investigate the potential use of these agents for wild rat pest population control. Liquid bait was made available to male (n ¼ 8 control; n ¼ 8 active) and female (n ¼ 8 control; n ¼ 8 active) Sprague Dawley rats (Rattus norvegicus) for oral consumption prior to breeding. Whereas, control bait-treated females produced normal-sized litters (10.0 6 1.7 pups/litter), treated females delivered no pups. Wild Norway male (n ¼ 20) and female (n ¼ 20) rats (Rattus norvegicus) were trapped, individually housed, and one group given free access to control bait, one group to active bait. Following three cycles of treatment-matched mating pairs, females consuming control bait (control) produced normal litter sizes (9.73 6 0.73 pups/litter). Females who had consumed active bait (treated) produced no litters on breeding cycles one and two; however, 2 of 10 females produced small litters on the third mating cycle. In a fourth breeding cycle, control females were crossmated with treated males, and treated females were crossmated with control males. In both groups, some dams produced litters, while others did not. The differences in response reflect a heterogeneity in return to cyclicity between females. These results suggest a potential approach to integrated pest management by compromising fertility, and could provide a novel alternative to traditional poisons for reducing populations of wild rat pests.

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Section: Wild-caught Ratsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

COMPROMISED FERTILITY IN FREE FEEDING OF WILD-CAUGHT NORWAY RATS (RATTUS NORVEGICUS) WITH A LIQUID BAIT CONTAINING 4-VINYLCYCLOHEXENE DIEPOXIDE AND TRIPTOLIDE

Witmer¹,

Raymond-Whish²,

Moulton³

et al. 2017

Journal of Zoo and Wildlife Medicine

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“…The inhibition of GC proliferation can also explain why E 2 level was decreased. There is also a study that confirmed that ovarian granulose cells may be one of the possible targets of the triptolide (Zhang et al., ).…”

Section: Discussionmentioning

confidence: 92%

Tripterygium Glycosides Impairs the Proliferation of Granulosa Cells and Decreases the Reproductive Outcomes in Female Rats

Cheng

Sun

et al. 2014

Birth Defects Research Pt B

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This study was carried out to investigate the impact of tripterygium glycosides (TGs) on ovarian function of female rats in vitro and in vivo. In vitro studies showed that TG induced cells decrease at G1 phase and inhibited cell proliferation in rat granulosa cells. In vivo, female rats were intragastrically administered with TG at the dose of 60 mg/kg/day for consecutive 50 days. TG caused a prolonged estrous cycle, and a significant reduction in ovarian index, serum E2 level, and numbers of secondary and antral follicles (p < 0.05) in these rats. A significant reduction of viable embryos was demonstrated in TG-treated female rats after mating (p < 0.01). Further, we observed observed the reduced expression level of TGF-β1 after TG treatment in vitro and in vivo. Moreover, the expression of Smad2 and AKT was also decreased after TG treatment. These results suggest that TG can impair ovarian function through Smads-mediated TGF-β1 signal pathway.

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“…Many clinical trials have also confirmed that TP has a good anti-inflammatory effect, especially in RA. Unfortunately, the hepatotoxic, nephrotoxic, and hematological toxic effects of TP limit its clinical applicability (Xue et al, 2011;Zhang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Efficient Delivery of Triptolide Plus a miR-30-5p Inhibitor Through the Use of Near Infrared Laser Responsive or CADY Modified MSNs for Efficacy in Rheumatoid Arthritis Therapeutics

Zhang

Wang

et al. 2020

Front. Bioeng. Biotechnol.

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Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease for which treatment focuses on suppressing an overactive immune system and maintaining the physiological balance of synovial fibroblasts (SFs). We found that miR-30-5p was highly expressed in rheumatoid arthritis synovial fibroblasts (RASFs). Subsequently, we predicted that phosphatidylinositol 3-kinase regulatory subunit 2 (PIK3R2) might be a putative target of miR-30-5p. Recent studies have reported that PIK3R2 can maintain the physiological homeostasis of RASFs. Therefore, miR-30-5p inhibitor has the potential to be used in the treatment of RA, but low levels of miR-30-5p inhibitor internalization affect its application. Triptolide (TP) is an effective drug in the treatment of RA but induces severe toxicity and has a narrow therapeutic window. In this study, the cell internalization performance of miR-30-5p inhibitor was improved by loading it into cell membrane penetrating peptide (CADY)-modified mesoporous silica nanoparticles (MSNs), and the toxicity of TP was decreased by loading it into a controlled drug release system based on MSNs. The nanodrug carrier was constructed by filling a phase-change material (PCM) of 1-tetradecanol and drugs into MSNs that could be triggered by an NIR laser with thermo-chemo combination RA therapy. Our results show that the miR-30-5p inhibitor-loaded MSNs@CADY significantly inhibited RASF proliferation and increased apoptosis. In addition, MSNs@PCM@TP under 808 nm laser irradiation were effective in downregulating immune system activation in an RA rat model. Finally, the results of a pharmacodynamics study showed that the combination of MSNs@CADY@miR-30-5p inhibitor and MSNs@PCM@TP under 808 nm laser significantly increased the effectiveness of RA treatment. These findings provide a novel understanding of RA pathogenesis and a theoretical basis for RA treatment.

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Effect of triptolide on estradiol release from cultured rat granulosa cells

Cited by 27 publications

References 40 publications

COMPROMISED FERTILITY IN FREE FEEDING OF WILD-CAUGHT NORWAY RATS (RATTUS NORVEGICUS) WITH A LIQUID BAIT CONTAINING 4-VINYLCYCLOHEXENE DIEPOXIDE AND TRIPTOLIDE

COMPROMISED FERTILITY IN FREE FEEDING OF WILD-CAUGHT NORWAY RATS (RATTUS NORVEGICUS) WITH A LIQUID BAIT CONTAINING 4-VINYLCYCLOHEXENE DIEPOXIDE AND TRIPTOLIDE

Tripterygium Glycosides Impairs the Proliferation of Granulosa Cells and Decreases the Reproductive Outcomes in Female Rats

Efficient Delivery of Triptolide Plus a miR-30-5p Inhibitor Through the Use of Near Infrared Laser Responsive or CADY Modified MSNs for Efficacy in Rheumatoid Arthritis Therapeutics

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