Effect of triphenyltin chloride on the release of histamine from mast cells

Abstract

The effects of triphenyltin chloride (TPTC) on the release of histamine from rat and mouse mast cells in vitro and in vivo were investigated. The results obtained are summarized as follows: (a) At doses between 1 and 3 mg/kg, TPTC inhibited the dye leakage due to passive cutaneous anaphylaxis mediated by IgE antibody in mouse ear. At the same doses, TPTC inhibited the swelling due to reversed cutaneous anaphylaxis mediated by IgG antibody in rat. However, TPTC did not affect dye leakage due to histamine, serotonin, and LTC4 in rat skin; (b) Histamine release by antigen and IgE antibody in rat peritoneal cavity was inhibited by the administration of TPTC at doses between 0.3 and 3 mg/kg; (c) Histamine release by calcium ionophore A 23187 from purified rat peritoneal mast cells in vitro was inhibited by TPTC at concentrations between 10(-7) and 10(-6) M. At the same concentration, TPTC, itself, caused neither the release of histamine nor any change in cell viability which was supported by the activity of lactate dehydrogenase (LDH) from purified rat peritoneal mast cells. All this evidence suggests that TPTC has an inhibitory effect on the release of histamine from mast cells without direct cytotoxicity.

Recovery of Purification-Associated Reduction in Antigen-Induced Histamine Release from Rat Peritoneal Mast Cells.

“…Purified peritoneal mast cells of rats usually release a small amount of histamine upon IgE-dependent stimulation, [19][20][21][22][23] therefore, investigators have sometimes employed phosphatidylserine to potentiate the response. [28][29][30] Previously, we demonstrated that the low IgE-dependent histamine release from purified mast cells could be recovered or potentiated by reconstitution with separated non-mast cells.…”

“…[19][20][21] Contact of mast cells with purification media does not result in the reduced histamine release, but the purification itself is crucial. Furthermore, the reduction of histamine release from purified mast cells seems to be a specific phenomenon for IgE-dependent mast cell activation, and the reduced histamine release is recovered by reconstituting with non-mast cells separated from mast cells.…”

“…[14][15][16][17][18] It has been noticed, however, that rat peritoneal mast cells purified by density gradient centrifugation release only a small amount of histamine upon challenge with antigen in comparison with non-purified preparations. [19][20][21] Contact of mast cells with purification media does not result in the reduced histamine release, but the purification itself is crucial. Furthermore, the reduction of histamine release from purified mast cells seems to be a specific phenomenon for IgE-dependent mast cell activation, and the reduced histamine release is recovered by reconstituting with non-mast cells separated from mast cells.…”