Effect of transplantation of cell suspension from embryonic nervous tissue and bone marrow on postischemic cerebral angiogenesis and restoration of limb motor function in rats with experimental ischemic stroke

Abstract

Ischemic stroke is one of the leading causes of mortality and disability worldwide. Dispite the progress of medical knowledge and technologies, the rate of permanent neurological impairment in patients after stroke remains high and effective strategy of restorative treatment is still at the stage of experimental development. Restoration of nervous system functions after stroke implies the activation of endogenous reparative processes, such as angiogenesis, using sources of regenerative medicine, including cell and tissue transplantation. Development of optimal and safe methods of neurotransplantation for stroke is one of the priorities of experimental research in this field. PURPOSE: to study the effect of post-stroke angiogenesis, stimulated by transplantation of cell suspension from embryonic nervous tissue (TCS-ENT) and bone marrow (TCS-BM), on restoration of motor functions in rats with experimental stroke. MATERIALS AND METHODS. 160 adult (3-4 months old) outbred albino rats weighing between 280-320 g were divided into groups and subgroups depending on the experimental procedure: with isolated middle cerebral artery occlusion (MCAO), intracerebral allotransplantation of cell suspension from embryonic nervous tissue (MCAO + TCS-ENT), intracerebral autotransplantation of cell suspension from bone marrow (MCAO + TCS-BM) or phosphate-buffered 0.9 % saline infusion (MCAO + PBS) on the 2 nd day after MCAO. MCAO was conducted using the modified method of intraluminal monofilament occlusion with blocking of collaterals. Volume of infarction zone was estimated using